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ECB-ART-44503
Data Brief 2015 Dec 12;6:715-21. doi: 10.1016/j.dib.2015.11.062.
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Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes.

Phanse S , Wan C , Borgeson B , Tu F , Drew K , Clark G , Xiong X , Kagan O , Kwan J , Bezginov A , Chessman K , Pal S , Cromar G , Papoulas O , Ni Z , Boutz DR , Stoilova S , Havugimana PC , Guo X , Malty RH , Sarov M , Greenblatt J , Babu M , Derry WB , Tillier ER , Wallingford JB , Parkinson J , Marcotte EM , Emili A .


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Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (PXD002319-PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].

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Genes referenced: LOC115919910 LOC583082

References [+] :
Andrews, A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. 1991, Pubmed