Sampath K , Robertson EJ .

Biotechnology and Biological Sciences Research Council

	Figure 1. Schematic of maternal sqt/nodal RNA expression in early zebrafish embryos. Sqt RNA localizes by the four-cell stage in one or two cells, which later form dorsal progenitors, marked by nuclear β-catenin and gsc expression.
	Figure 2. Schematic of sqt DLE sequence and structure motif. (a) Schematic of sqt exon 3 and the 3′-UTR, showing the dorsal localization element (DLE; green shading), which includes a single-stranded motif (pink box) and a short stem-loop structure. (b) The sequence of the single-stranded motif (pink highlighted region) and the stem-loop structure of the hairpin are shown.
	Figure 3. Schematic of zebrafish ybx1 and sqt/nodal mutant phenotypes. In sqt mutants gsc expression is initiated but not maintained, whereas in Mybx1 mutants, sqt RNA is mis-localized and gsc is expressed prematurely in the expanded yolk syncytial layer (YSL). DIC images show the phenotypes of wild-type or mutant embryos.
	Figure 4. Model of translational repression of Nodal pathway components. (a) In early zebrafish embryos, Ybx1 represses squint/nodal translation by binding to the translation pre-initiation complex proteins and the squint DLE (solid blue box). Squint/Nodal translation is activated from late blastula stages by unknown factors. (b) In vegetal cells of frog embryos, Bic-C represses xCR1 translation by binding to the translation pre-initiation complex proteins and the xCR1 TCE (solid green box). In animal cap cells, xCR1 translation is activated in the absence of Bic-C. (c) miRNA-378a-5p (black comb) binds to the 3′-UTR (solid red box) of human Nodal RNA and unknown factors (?) to repress Nodal translation in the human placenta. In the absence of miRNA-378a-5p, nodal translation is activated.

Aanes, Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition. 2011, Pubmed

Aanes, Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition. 2011, Pubmed
Agrawal, Exome sequencing of head and neck squamous cell carcinoma reveals inactivating mutations in NOTCH1. 2011, Pubmed
Beck, Extraembryonic proteases regulate Nodal signalling during gastrulation. 2002, Pubmed
Ben-Haim, The nodal precursor acting via activin receptors induces mesoderm by maintaining a source of its convertases and BMP4. 2006, Pubmed
Bertero, Activin/nodal signaling and NANOG orchestrate human embryonic stem cell fate decisions by controlling the H3K4me3 chromatin mark. 2015, Pubmed
Bisgrove, Regulation of midline development by antagonism of lefty and nodal signaling. 1999, Pubmed
Bouvet, Sequence-specific RNA recognition by the Xenopus Y-box proteins. An essential role for the cold shock domain. 1995, Pubmed
Branford, Lefty-dependent inhibition of Nodal- and Wnt-responsive organizer gene expression is essential for normal gastrulation. 2002, Pubmed
Camus, Absence of Nodal signaling promotes precocious neural differentiation in the mouse embryo. 2006, Pubmed
Cao, Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation. 2008, Pubmed
Cavalieri, Early asymmetric cues triggering the dorsal/ventral gene regulatory network of the sea urchin embryo. 2014, Pubmed , Echinobase
Ceman, Identification of mouse YB1/p50 as a component of the FMRP-associated mRNP particle. 2000, Pubmed
Cheng, The lefty-related factor Xatv acts as a feedback inhibitor of nodal signaling in mesoderm induction and L-R axis development in xenopus. 2000, Pubmed
Choi, Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430. 2007, Pubmed
Collignon, Relationship between asymmetric nodal expression and the direction of embryonic turning. 1996, Pubmed
Conlon, A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. 1994, Pubmed
Constam, Running the gauntlet: an overview of the modalities of travel employed by the putative morphogen Nodal. 2009, Pubmed
D'Andrea, Cripto promotes A-P axis specification independently of its stimulatory effect on Nodal autoinduction. 2008, Pubmed
Dick, Cloning and characterization of zebrafish smad2, smad3 and smad4. 2000, Pubmed
Dorey, A novel Cripto-related protein reveals an essential role for EGF-CFCs in Nodal signalling in Xenopus embryos. 2006, Pubmed
Duboc, Nodal and BMP2/4 pattern the mesoderm and endoderm during development of the sea urchin embryo. 2010, Pubmed , Echinobase
Erter, Zebrafish nodal-related 2 encodes an early mesendodermal inducer signaling from the extraembryonic yolk syncytial layer. 1998, Pubmed
Evdokimova, Translational activation of snail1 and other developmentally regulated transcription factors by YB-1 promotes an epithelial-mesenchymal transition. 2009, Pubmed
Fei, Smad2 mediates Activin/Nodal signaling in mesendoderm differentiation of mouse embryonic stem cells. 2010, Pubmed
Feldman, Zebrafish organizer development and germ-layer formation require nodal-related signals. 1998, Pubmed
Feldman, Lefty antagonism of Squint is essential for normal gastrulation. 2002, Pubmed
Garg, Cloning of zebrafish activin type IIB receptor (ActRIIB) cDNA and mRNA expression of ActRIIB in embryos and adult tissues. 1999, Pubmed
Gilligan, Conservation defines functional motifs in the squint/nodal-related 1 RNA dorsal localization element. 2011, Pubmed
Gore, Localization of transcripts of the zebrafish morphogen Squint is dependent on egg activation and the microtubule cytoskeleton. 2002, Pubmed
Gore, The zebrafish dorsal axis is apparent at the four-cell stage. 2005, Pubmed
Grande, Nodal signalling is involved in left-right asymmetry in snails. 2009, Pubmed , Echinobase
Granier, Nodal cis-regulatory elements reveal epiblast and primitive endoderm heterogeneity in the peri-implantation mouse embryo. 2011, Pubmed
Gritsman, Nodal signaling patterns the organizer. 2000, Pubmed
Gualerzi, Transcriptional and post-transcriptional control of cold-shock genes. 2003, Pubmed
Hagos, Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish. 2007, Pubmed
Hagos, The role of maternal Activin-like signals in zebrafish embryos. 2007, Pubmed
Hudson, Patterning across the ascidian neural plate by lateral Nodal signalling sources. 2005, Pubmed
Iratni, Inhibition of excess nodal signaling during mouse gastrulation by the transcriptional corepressor DRAP1. 2002, Pubmed
James, TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells. 2005, Pubmed
Kofron, New roles for FoxH1 in patterning the early embryo. 2004, Pubmed
Kumari, An essential role for maternal control of Nodal signaling. 2013, Pubmed , Echinobase
Lee, Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins. 1994, Pubmed
Le Good, Nodal stability determines signaling range. 2005, Pubmed
Long, The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry. 2003, Pubmed
Luo, MicroRNA-378a-5p promotes trophoblast cell survival, migration and invasion by targeting Nodal. 2012, Pubmed
Maher-Laporte, Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain. 2010, Pubmed
Marello, Binding of Xenopus oocyte masking proteins to mRNA sequences. 1992, Pubmed
Martello, MicroRNA control of Nodal signalling. 2007, Pubmed
Meno, Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. 1999, Pubmed
Mesnard, Nodal specifies embryonic visceral endoderm and sustains pluripotent cells in the epiblast before overt axial patterning. 2006, Pubmed
Moss, The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA. 1997, Pubmed
Müller, Characterization of zebrafish smad1, smad2 and smad5: the amino-terminus of smad1 and smad5 is required for specific function in the embryo. 1999, Pubmed
Munir, Nodal and ALK7 inhibit proliferation and induce apoptosis in human trophoblast cells. 2004, Pubmed
Murray, Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos. 1991, Pubmed
Murray, Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins. 1992, Pubmed
Nadeem, Nodal signals through activin receptor-like kinase 7 to inhibit trophoblast migration and invasion: implication in the pathogenesis of preeclampsia. 2011, Pubmed
Nakashima, A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli. 1996, Pubmed
Nelson, Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression. 2014, Pubmed
Ogawa, Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells. 2007, Pubmed
Osada, Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1. 2000, Pubmed
Paranjpe, A genome-wide survey of maternal and embryonic transcripts during Xenopus tropicalis development. 2013, Pubmed
Pogoda, The zebrafish forkhead transcription factor FoxH1/Fast1 is a modulator of nodal signaling required for organizer formation. 2000, Pubmed
Range, Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-beta related to Vg1. 2007, Pubmed , Echinobase
Raya, Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos. 2003, Pubmed
Rebagliati, Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry. 1998, Pubmed
Renucci, An activated form of type I serine/threonine kinase receptor TARAM-A reveals a specific signalling pathway involved in fish head organiser formation. 1996, Pubmed
Robertson, Dose-dependent Nodal/Smad signals pattern the early mouse embryo. 2014, Pubmed
Robertson, Control of early anterior-posterior patterning in the mouse embryo by TGF-beta signalling. 2003, Pubmed
Rosa, The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection. 2009, Pubmed
Röttinger, Nodal signaling is required for mesodermal and ventral but not for dorsal fates in the indirect developing hemichordate, Ptychodera flava. 2015, Pubmed
Sallés, Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs. 1994, Pubmed
Sampath, Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling. 1998, Pubmed
Sarkar, Activin/nodal signaling switches the terminal fate of human embryonic stem cell-derived trophoblasts. 2015, Pubmed
Schier, Nodal morphogens. 2009, Pubmed
Shen, Nodal signaling: developmental roles and regulation. 2007, Pubmed
Simon, Cytoplasmic polyadenylation of activin receptor mRNA and the control of pattern formation in Xenopus development. 1996, Pubmed
Sirotkin, Fast1 is required for the development of dorsal axial structures in zebrafish. 2000, Pubmed
Sun, Activation and roles of ALK4/ALK7-mediated maternal TGFbeta signals in zebrafish embryo. 2006, Pubmed
Tanaka, YB-1 binds to GluR2 mRNA and CaM1 mRNA in the brain and regulates their translational levels in an activity-dependent manner. 2010, Pubmed
Tanaka, CiYB1 is a major component of storage mRNPs in ascidian oocytes: implications in translational regulation of localized mRNAs. 2004, Pubmed
Tanegashima, Expression cloning of Xantivin, a Xenopus lefty/antivin-related gene, involved in the regulation of activin signaling during mesoderm induction. 2000, Pubmed
Tao, Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos. 2005, Pubmed
ten Dijke, New insights into TGF-beta-Smad signalling. 2004, Pubmed
Thisse, Antivin, a novel and divergent member of the TGFbeta superfamily, negatively regulates mesoderm induction. 1999, Pubmed
Tian, The pro-domain of the zebrafish Nodal-related protein Cyclops regulates its signaling activities. 2008, Pubmed
Topczewska, Embryonic and tumorigenic pathways converge via Nodal signaling: role in melanoma aggressiveness. 2006, Pubmed
Tran, Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. 2012, Pubmed
Uchiumi, YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation. 2006, Pubmed
Vallier, Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. 2005, Pubmed
van Boxtel, A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain. 2015, Pubmed
Watanabe, Nodal signalling determines biradial asymmetry in Hydra. 2014, Pubmed
Wilhelm, Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes. 2000, Pubmed
Wills, E2a is necessary for Smad2/3-dependent transcription and the direct repression of lefty during gastrulation. 2015, Pubmed
Xu, Maternal Eomesodermin regulates zygotic nodal gene expression for mesendoderm induction in zebrafish embryos. 2014, Pubmed
Yabe, FRL-1, a member of the EGF-CFC family, is essential for neural differentiation in Xenopus early development. 2003, Pubmed
Yaguchi, A Wnt-FoxQ2-nodal pathway links primary and secondary axis specification in sea urchin embryos. 2008, Pubmed , Echinobase
Yamanaka, The CspA family in Escherichia coli: multiple gene duplication for stress adaptation. 1998, Pubmed
Yu, AmphiFoxQ2, a novel winged helix/forkhead gene, exclusively marks the anterior end of the amphioxus embryo. 2003, Pubmed
Zhang, Determinants of RNA binding and translational repression by the Bicaudal-C regulatory protein. 2014, Pubmed
Zhang, Spatially restricted translation of the xCR1 mRNA in Xenopus embryos. 2009, Pubmed
Zhang, Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation. 1998, Pubmed
Zhang, Bicaudal-C spatially controls translation of vertebrate maternal mRNAs. 2013, Pubmed
Zhang, Zebrafish Dpr2 inhibits mesoderm induction by promoting degradation of nodal receptors. 2004, Pubmed
Zhou, Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. 1993, Pubmed