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Data Brief
2015 Sep 09;5:155-60. doi: 10.1016/j.dib.2015.09.001.
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Data in support of quantitative proteomics to identify potential virulence regulators in Paracoccidioides brasiliensis isolates.
Tashima AK
,
Castilho DG
,
Chaves AF
,
Xander P
,
Zelanis A
,
Batista WL
.
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Paracoccidioides genus are the etiologic agents of paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America. Few virulence factors have been identified in these fungi. This paper describes support data from the quantitative proteomics of Paracoccidioides brasiliensis attenuated and virulent isolates [1]. The protein compositions of two isolates of the Pb18 strain showing distinct infection profiles were quantitatively assessed by stable isotopic dimethyl labeling and proteomic analysis. The mass spectrometry and the analysis dataset have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with identifier PXD000804.
Fig. 1. Schematic representation of the experimental strategy for proteomic analysis of alterations in Paracoccidioides brasiliensis (isolate Pb18) with different degrees of virulence. Virulent Pb18 was obtained by passage in animals and attenuated Pb18 was reached by successive sub-culturing in vitro. Before protein extraction, virulent and attenuated Pb18 were grown in MM or RM for 5 days. Then, the proteins were extracted, denatured with 4 M GuHCl, reduced with 5 mM DTT, alkylated with 15 mM iodoacetamide and digested separately with trypsin. Tryptic peptides were differentially labeled via stable-isotope dimethyl labeling. Samples (vPb18 and aPb18) were mixed, desalted and dried. Peptides were redissolved in formic acid and subjected to nanoLC system coupled with LTQ-Orbitrap mass spectrometer for MS analysis. Raw data were acquired by Xcalibur software, and were searched against the Paracoccidioides brasiliensis strain Pb18 database using PEAKS Studio 7. qPCR assays were performed to validate the data.
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