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BMC Complement Altern Med
2015 Feb 05;15:14. doi: 10.1186/s12906-015-0533-7.
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Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats.
Fahmy SR.
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BACKGROUND: Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats.
METHODS: Fifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight).
RESULTS: The HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities.
CONCLUSION: The present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.
Figure 2.
High-performance liquid chromatographic analysis of
Holothuria arenicola
extract.
Figure 4.
Histological study of picrosirius red -stained liver sections (200×) of Sham, BDL and
Holothuria arenicola
extract (HaE) treated rats. Rats were grouped: group 1, rats treated for 7Â days; group 2, rats treated for 14Â days and group 3, rats treated for 28Â days. Liver sections from: (A) Control (Sham operated) rats; (B) bile duct-ligated rats; (C) bile duct-ligated rats and treated with Holothuria arenicola extract (HaE). Arrows indicated collagen deposition stained by picrosirius red.
Aebi,
Catalase in vitro.
1984,
Pubmed Altug,
Effect of ginger extract on liver damage in experimental obstructive jaundice produced by main bile duct ligation.
2013,
Pubmed Alvaro,
The function of alkaline phosphatase in the liver: regulation of intrahepatic biliary epithelium secretory activities in the rat.
2000,
Pubmed Assimakopoulos,
Evidence for intestinal oxidative stress in patients with obstructive jaundice.
2006,
Pubmed Aykaç,
The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats.
1985,
Pubmed Ayvaz,
The effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in rats.
2013,
Pubmed Belfield,
Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine.
1971,
Pubmed Bessa,
Heme oxygenase-1 mRNA expression in egyptian patients with chronic liver disease.
2012,
Pubmed Crocenzi,
Localization status of hepatocellular transporters in cholestasis.
2012,
Pubmed Damnjanović,
Correlation of inflammation parameters and biochemical markers of cholestasis with the intensity of lipid peroxidation in patients with choledocholithiasis.
2013,
Pubmed Dinarello,
Interleukin-1 and the pathogenesis of the acute-phase response.
1984,
Pubmed Esmat,
Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats.
2013,
Pubmed
,
Echinobase Fahmy,
Curative effect of the Egyptian marine Erugosquilla massavensis extract on carbon tetrachloride-induced oxidative stress in rat liver and erythrocytes.
2011,
Pubmed Faubion,
Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas.
1999,
Pubmed Fratto,
Targeting EGFR in bilio-pancreatic and liver carcinoma.
2011,
Pubmed Friedman,
Mechanisms of hepatic fibrogenesis.
2008,
Pubmed Friedman,
Liver fibrosis -- from bench to bedside.
2003,
Pubmed Fuentes-Broto,
Melatonin protects against taurolithocholic-induced oxidative stress in rat liver.
2010,
Pubmed Gaté,
Impact of dietary supplement of Crassostrea gigas extract (JCOE) on glutathione levels and glutathione S-transferase activity in rat tissues.
1998,
Pubmed Habig,
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.
1974,
Pubmed Han,
Aqueous extract of Artemisia iwayomogi Kitamura attenuates cholestatic liver fibrosis in a rat model of bile duct ligation.
2012,
Pubmed Harmatz,
The effect of bile duct obstruction on the clearance of circulating IgA immune complexes.
1984,
Pubmed Jaeschke,
Reactive oxygen and mechanisms of inflammatory liver injury: Present concepts.
2011,
Pubmed Jayaraman,
Impact of Essentiale L on ethanol-induced changes in rat brain and erythrocytes.
2008,
Pubmed Jin,
Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis.
2013,
Pubmed Jüngst,
Intrahepatic cholestasis in common chronic liver diseases.
2013,
Pubmed Kim,
CGX, a multiple herbal drug, improves cholestatic liver fibrosis in a bile duct ligation-induced rat model.
2013,
Pubmed Kim,
Protective effect of the aqueous extract from the root of Platycodon grandiflorum on cholestasis-induced hepatic injury in mice.
2012,
Pubmed Koyama,
Hepatoprotective effect of a hot-water extract from the edible thorny oyster Spondylus varius on carbon tetrachloride-induced liver injury in mice.
2006,
Pubmed Lee,
Adaptive regulation of bile salt transporters in kidney and liver in obstructive cholestasis in the rat.
2001,
Pubmed Lee,
Herb medicine Yin-Chen-Hao-Tang ameliorates hepatic fibrosis in bile duct ligation rats.
2007,
Pubmed Li,
Liver fibrogenesis and the role of hepatic stellate cells: new insights and prospects for therapy.
1999,
Pubmed Liu,
Suppression of nuclear factor erythroid 2-related factor 2 via extracellular signal-regulated kinase contributes to bleomycin-induced oxidative stress and fibrogenesis.
2013,
Pubmed Mustafa,
Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants.
2010,
Pubmed Nishikimi,
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen.
1972,
Pubmed Noble,
A study of preoperative factors associated with a poor outcome following laparoscopic bile duct exploration.
2011,
Pubmed Ohkawa,
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.
1979,
Pubmed Olteanu,
The effects of chitosan and low dose dexamethasone on extrahepatic cholestasis after bile duct ligation in Wistar rats.
2012,
Pubmed Padda,
Liver test patterns in patients with acute calculous cholecystitis and/or choledocholithiasis.
2009,
Pubmed Pastor,
Antioxidant enzyme status in biliary obstructed rats: effects of N-acetylcysteine.
1997,
Pubmed REITMAN,
A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
1957,
Pubmed Salas,
Genistein decreases liver fibrosis and cholestasis induced by prolonged biliary obstruction in the rat.
2007,
Pubmed Sanzgiri,
Uptake, distribution, and elimination of carbon tetrachloride in rat tissues following inhalation and ingestion exposures.
1997,
Pubmed Schmucker,
Hepatic injury induced by bile salts: correlation between biochemical and morphological events.
1990,
Pubmed Sener,
Hepatic fibrosis in biliary-obstructed rats is prevented by Ginkgo biloba treatment.
2005,
Pubmed Sharma,
Hepatoprotective potential of aqueous extract of Butea monosperma against CCl(4) induced damage in rats.
2011,
Pubmed Sokolovic,
The effect of ursodeoxycholic acid on oxidative stress level and DNase activity in rat liver after bile duct ligation.
2013,
Pubmed Somi,
Lipoic acid prevents hepatic and intestinal damage induced by obstruction of the common bile duct in rats.
2013,
Pubmed Suzuki,
Rat hypoplastic kidney (hpk/hpk) induces renal anemia, hyperparathyroidism, and osteodystrophy at the end stage of renal failure.
1998,
Pubmed Svegliati Baroni,
Fibrogenic effect of oxidative stress on rat hepatic stellate cells.
1998,
Pubmed Szabo,
Glutathione, protein sulfhydryls and cysteine proteases in gastric mucosal injury and protection.
1992,
Pubmed Tokaç,
Protective effects of curcumin against oxidative stress parameters and DNA damage in the livers and kidneys of rats with biliary obstruction.
2013,
Pubmed Tsuchiya,
Overexpression of rat heat shock protein 70 is associated with reduction of early mitochondrial cytochrome C release and subsequent DNA fragmentation after permanent focal ischemia.
2003,
Pubmed van den Heuvel,
The international validation of a fixed-dose procedure as an alternative to the classical LD50 test.
1990,
Pubmed Webster,
Cyclic adenosine monophosphate-mediated protection against bile acid-induced apoptosis in cultured rat hepatocytes.
1998,
Pubmed Whitehead,
Statistical evaluation of the fixed-dose procedure.
1992,
Pubmed Xu,
Protective effects of chlorogenic acid on acute hepatotoxicity induced by lipopolysaccharide in mice.
2010,
Pubmed Zhu,
Suppression of thioredoxin system contributes to silica-induced oxidative stress and pulmonary fibrogenesis in rats.
2013,
Pubmed
