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The capacity to withstand and repair DNA damage differs among species and plays a role in determining an organism''s resistance to genotoxicity, life history, and susceptibility to disease. Environmental stressors that affect organisms at the genetic level are of particular concern in ecotoxicology due to the potential for chronic effects and trans-generational impacts on populations. Echinoderms are valuable organisms to study the relationship between DNA repair and resistance to genotoxic stress due to their history and use as ecotoxicological models, little evidence of senescence, and few reported cases of neoplasia. Coelomocytes (immune cells) have been proposed to serve as sensitive bioindicators of environmental stress and are often used to assess genotoxicity; however, little is known about how coelomocytes from different echinoderm species respond to genotoxic stress. In this study, DNA damage was assessed (by Fast Micromethod) in coelomocytes of four echinoderm species (sea urchins Lytechinus variegatus, Echinometra lucunter lucunter, and Tripneustes ventricosus, and a sea cucumber Isostichopus badionotus) after acute exposure to H2O2 (0-100 mM) and UV-C (0-9999 J/m2), and DNA repair was analyzed over a 24-hour period of recovery. Results show that coelomocytes from all four echinoderm species have the capacity to repair both UV-C and H2O2-induced DNA damage; however, there were differences in repair capacity between species. At 24 hours following exposure to the highest concentration of H2O2 (100 mM) and highest dose of UV-C (9999 J/m2) cell viability remained high (>94.6 ± 1.2%) but DNA repair ranged from 18.2 ± 9.2% to 70.8 ± 16.0% for H2O2 and 8.4 ± 3.2% to 79.8 ± 9.0% for UV-C exposure. Species-specific differences in genotoxic susceptibility and capacity for DNA repair are important to consider when evaluating ecogenotoxicological model organisms and assessing overall impacts of genotoxicants in the environment.
???displayArticle.pubmedLink???
25229547
???displayArticle.pmcLink???PMC4168213 ???displayArticle.link???PLoS One
Figure 1. Dose/concentration response in echinoderm coelomocytes.Increase in DNA damage (strand scission factor, SSF, Fast Micromethod) with increasing concentration of H2O2 (A, C, E, and G) or dose of UV-C (B, D, F, and H) after acute exposure of coelomocytes from T. ventricosus (A and B, n = 5), L. variegatus (C and D, n = 11–12), E. l. lucunter (E and F, n = 6–7), and I. badionotus (G and H, n = 8). Data are means ± s.e.m.
Anderson,
Linking genotoxic responses and reproductive success in ecotoxicology.
1994, Pubmed,
Echinobase
Anderson,
Linking genotoxic responses and reproductive success in ecotoxicology.
1994,
Pubmed
,
Echinobase
Azqueta,
Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay--a methodological overview.
2013,
Pubmed
Azqueta,
DNA oxidation: investigating its key role in environmental mutagenesis with the comet assay.
2009,
Pubmed
Belfiore,
Effects of contaminants on genetic patterns in aquatic organisms: a review.
2001,
Pubmed
Bodnar,
Marine invertebrates as models for aging research.
2009,
Pubmed
,
Echinobase
Bolognesi,
Micronucleus assay in aquatic animals.
2011,
Pubmed
Branco,
The impact of rising sea temperature on innate immune parameters in the tropical subtidal sea urchin Lytechinus variegatus and the intertidal sea urchin Echinometra lucunter.
2013,
Pubmed
,
Echinobase
Canty,
Linking genotoxic responses with cytotoxic and behavioural or physiological consequences: differential sensitivity of echinoderms (Asterias rubens) and marine molluscs (Mytilus edulis).
2009,
Pubmed
,
Echinobase
Cheung,
An evaluation of the relative sensitivity of two marine bivalve mollusc species using the Comet assay.
2006,
Pubmed
Collins,
Measuring oxidative damage to DNA and its repair with the comet assay.
2014,
Pubmed
Cooke,
Oxidative DNA damage: mechanisms, mutation, and disease.
2003,
Pubmed
Costello,
Global coordination and standardisation in marine biodiversity through the World Register of Marine Species (WoRMS) and related databases.
2013,
Pubmed
Dallas,
Oxidative DNA damage may not mediate Ni-induced genotoxicity in marine mussels: assessment of genotoxic biomarkers and transcriptional responses of key stress genes.
2013,
Pubmed
Depledge,
The ecotoxicological significance of genotoxicity in marine invertebrates.
1998,
Pubmed
Dubrova,
Radiation-induced transgenerational instability.
2003,
Pubmed
Ebert,
Longevity and lack of senescence in the red sea urchin Strongylocentrotus franciscanus.
2008,
Pubmed
,
Echinobase
Enoch,
Cellular responses to DNA damage: cell-cycle checkpoints, apoptosis and the roles of p53 and ATM.
1995,
Pubmed
Friedberg,
DNA damage and repair.
2003,
Pubmed
Henle,
Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide.
1997,
Pubmed
Hernroth,
Possibility of mixed progenitor cells in sea star arm regeneration.
2010,
Pubmed
,
Echinobase
Holm,
Induced cell proliferation in putative haematopoietic tissues of the sea star, Asterias rubens (L.).
2008,
Pubmed
,
Echinobase
Hook,
Genotoxicant induced DNA damage and repair in early and late developmental stages of the grass shrimp Paleomonetes pugio embryo as measured by the comet assay.
2004,
Pubmed
Hose,
Potential uses of sea urchin embryos for identifying toxic chemicals: description of a bioassay incorporating cytologic, cytogenetic and embryologic endpoints.
1985,
Pubmed
,
Echinobase
Jha,
Genotoxicological studies in aquatic organisms: an overview.
2004,
Pubmed
Kirkwood,
Understanding the odd science of aging.
2005,
Pubmed
Kleinjans,
Ecogenotoxicology: the evolving field.
2002,
Pubmed
Klerks,
Quantitative genetics approaches to study evolutionary processes in ecotoxicology; a perspective from research on the evolution of resistance.
2011,
Pubmed
Kolarević,
Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay.
2013,
Pubmed
Lamare,
DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species.
2006,
Pubmed
,
Echinobase
Le Bouffant,
Sea urchin embryo as a model for analysis of the signaling pathways linking DNA damage checkpoint, DNA repair and apoptosis.
2007,
Pubmed
,
Echinobase
Lesser,
Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos.
2003,
Pubmed
,
Echinobase
Long,
Apoptotic and necrotic mechanisms of stress-induced human lens epithelial cell death.
2004,
Pubmed
Loram,
Sea urchin coelomocytes are resistant to a variety of DNA damaging agents.
2012,
Pubmed
,
Echinobase
Matranga,
Cellular and biochemical responses to environmental and experimentally induced stress in sea urchin coelomocytes.
2000,
Pubmed
,
Echinobase
McClung,
Calpain-1 is required for hydrogen peroxide-induced myotube atrophy.
2009,
Pubmed
Misovic,
Short-term exposure to UV-A, UV-B, and UV-C irradiation induces alteration in cytoskeleton and autophagy in human keratinocytes.
2013,
Pubmed
Murakami,
Multiplex stress resistance in cells from long-lived dwarf mice.
2003,
Pubmed
Pinsino,
Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Adriatic Sea, Italy.
2008,
Pubmed
,
Echinobase
Ramos-Espinosa,
Differential DNA damage response to UV and hydrogen peroxide depending of differentiation stage in a neuroblastoma model.
2012,
Pubmed
Rastogi,
Molecular mechanisms of ultraviolet radiation-induced DNA damage and repair.
2010,
Pubmed
Reinardy,
Changes in expression profiles of genes associated with DNA repair following induction of DNA damage in larval zebrafish Danio rerio.
2013,
Pubmed
Reinecke,
The comet assay as biomarker of heavy metal genotoxicity in earthworms.
2004,
Pubmed
Ribeiro,
Contaminant driven genetic erosion and associated hypotheses on alleles loss, reduced population growth rate and increased susceptibility to future stressors: an essay.
2013,
Pubmed
Robert,
Comparative study of tumorigenesis and tumor immunity in invertebrates and nonmammalian vertebrates.
2010,
Pubmed
Saco-Alvarez,
Methodological basis for the optimization of a marine sea-urchin embryo test (SET) for the ecological assessment of coastal water quality.
2010,
Pubmed
,
Echinobase
Salmon,
Fibroblasts from naked mole-rats are resistant to multiple forms of cell injury, but sensitive to peroxide, ultraviolet light, and endoplasmic reticulum stress.
2008,
Pubmed
Sinha,
UV-induced DNA damage and repair: a review.
2002,
Pubmed
Ungvari,
Extreme longevity is associated with increased resistance to oxidative stress in Arctica islandica, the longest-living non-colonial animal.
2011,
Pubmed
Ungvari,
Resistance to genotoxic stresses in Arctica islandica, the longest living noncolonial animal: is extreme longevity associated with a multistress resistance phenotype?
2013,
Pubmed
Valavanidis,
Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants.
2006,
Pubmed
Würgler,
Environmental effects of genotoxins (eco-genotoxicology).
1992,
Pubmed
