Wei Sheng Yan Jiu 2014 Jul 01;434:598-602.

[Antitumor effect of Stichopus japonicus acidic mucopolysaccharide combined with fluorouracil in mice bearing neoplasia of H22 hepatoma cells].

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OBJECTIVE: To observe the antitumor effect of Stihopus japonicus acidic mucopolysaccharide combined with fluorouracil in mice bearing neoplasia of H22 hepatoma cells, and study the mechanism. METHODS: Inoculate the H22 liver cancer cells in the right forelimbs axillary region subcutaneous of 50 Kunming mice, and then randomly divide the mice into 5 groups, including negative control group, 5-FU group, SJAMP group and two combined groups. Tumor histopathological changes were examined by HE. The expressions of PCNA protein level, P53, P21, Cyclin D1 and CDK4 protein and mRNA levels in the H22 tumor tissues were detected by immunohistochemical and Real-time PCR methods. RESULTS: Compared with negative control group, the weights of the H22 tumor tissues in other groups are decreased statistically significant (P < 0.05). The anti-tumor rates of 5-FU group and the two combined groups are all exceed 50%. The anti-tumor rate of the high dose of 5-FU combined SJAMP group is reach up to 62.73%. HE staining showed that except negative control group, other groups tumor cells were less, arranged loosely, karyopyknosis and necrotic area were increased. According to the results of Immunohistochemistry and Real-time PCR, the expressions of PCNA protein, P53, Cyclin D1 and CDK4 proteins and mRNAs of the medication administration groups are decreased obviously compared with negative control group (P < 0.05), expressions of P21 proteins are higher than negative control group (P < 0.05), the expressions of related genes mRNA and proteins changes are more remarkable in high dose of 5-FU combined SJAMP group (P < 0.05). CONCLUSION: SJAMP and 5-FU can via decrease the expressions of PCNA protein, P53, Cyclin D1 and CDK4 proteins and mRNAs, increase the expressions of P21 proteins and mRNAs, to inhibit the growth of H22 tumor in mice. And the combination of SJAMP and 5-FU shows synergistic effect which can strengthen the ability of antitumor obviously.

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Genes referenced: cdk6 LOC115918456 LOC592256