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Food Sci Nutr
2013 Jan 01;11:83-9. doi: 10.1002/fsn3.10.
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Sulfated glycosaminoglycans from crown-of-thorns Acanthaster planci - extraction and quantification analysis.
Bahrom NA
,
Sirajudeen K
,
Yip GW
,
Latiff AA
,
Ghazali FC
.
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In this article, the novel inventive steps for the extraction and quantification of sulfated glycosaminoglycan (GAG) from Acanthaster planci starfish, generally known as crown-of-thorns (COT), are reported. Starfish have been implicated with collagenous distributions within their body anatomy, thus making it a prima facie fact searching for the possibility that GAGs can be isolated from COT. In this study, total-, N-, and O-sulfated GAGs were extracted from three anatomical regions of the COT (integument, internal tissue, and coelomic fluid) and comparison was made. The result showed that body region of COT seemed to contain higher amount of sulfated GAGs as opposed to the arm region (55.79 ± 0.65 μg/mg was the highest amount in the body extracted from its coelomic fluid and 32.28 ± 3.14 μg/mg was the highest amount in the arm extracted from its internal tissue). COT''s integument and coelomic fluid from its body region possessed the highest total of sulfated GAGs content with no significant difference (P < 0.05) between the two. All GAGs from COT comprised a higher percentage of N-sulfated GAGs than its counterpart, the O-sulfated GAGs. When compared with a similar previous study that used sea cucumbers as the sulfated GAGs source, COT possessed more total sulfated GAGs content per milligram as compared with the sea cucumber generally. This result seems to unveil this marine species'' advantage per se pertaining to GAGs extraction biomass applicability. Thus, COT could now be the better alternative source for production technology of total-, N-, and O-sulfated GAGs.
Figure 1. Linear calibration curve using chondroitin 4-sulfate as a standard. This calibration curve was performed by plotting the mean of three sets of standard solution after subtracting the mean duplicate absorbance readings of the reagent blank.
Figure 2. Total sulfated glycosaminoglycan (GAG) content (μg/mg) of three different anatomical parts of crown-of-thorns (COT)'s body and arm. Each analysis was performed in triplicates and data presented in mean ± standard error of mean (SEM). (a,b), significant difference between the same anatomical parts of different region, P < 0.05; (aa,bb), significant difference between the anatomical parts from body region, P < 0.05; (cc), significant difference between the anatomical parts from arm region, P < 0.05.
Figure 3. Total N-sulfated glycosaminoglycan (GAG) content (μg/mg) of three different anatomical parts of crown-of-thorns (COT)'s body and arm. Each analysis was performed in triplicates and data presented in mean ± standard error of mean (SEM). (a,b,c), significant difference between the same anatomical parts of different region, P < 0.05; (aa,bb), significant difference between the anatomical parts from body region, P < 0.05; (cc), significant difference between the anatomical parts from arm region, P < 0.05.
Figure 4. Total O-sulfated glycosaminoglycan (GAG) content (μg/mg) of three different anatomical parts of crown-of-thorns (COT)'s body and arm. Each analysis was performed in triplicates and data presented in mean ± standard error of mean (SEM). (a,b,c), significant difference between the same anatomical parts of different region, P < 0.05; (aa), significant difference between the anatomical parts from body region, P < 0.05; (bb,cc), significant difference between the anatomical parts from arm region, P < 0.05.
Alexopoulou,
Syndecans in wound healing, inflammation and vascular biology.
2007, Pubmed
Alexopoulou,
Syndecans in wound healing, inflammation and vascular biology.
2007,
Pubmed
Barbosa,
Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies.
2003,
Pubmed
Esko,
Molecular diversity of heparan sulfate.
2001,
Pubmed
Fujita,
HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development.
2010,
Pubmed
,
Echinobase
Kariya,
Structure of fucose branches in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus.
1997,
Pubmed
,
Echinobase
Lamoureux,
Glycosaminoglycans as potential regulators of osteoprotegerin therapeutic activity in osteosarcoma.
2009,
Pubmed
Landeira-Fernandez,
A sulfated polysaccharide from the sarcoplasmic reticulum of sea cucumber smooth muscle is an endogenous inhibitor of the Ca(2+)-ATPase.
2000,
Pubmed
,
Echinobase
Ledin,
Heparan sulfate structure in mice with genetically modified heparan sulfate production.
2004,
Pubmed
Lee,
Suppression of tumor growth by a new glycosaminoglycan isolated from the African giant snail Achatina fulica.
2003,
Pubmed
Masre,
Quantitative analysis of sulphated glycosaminoglycans content of Malaysian sea cucumber Stichopus hermanni and Stichopus vastus.
2012,
Pubmed
,
Echinobase
Medeiros,
Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates.
2000,
Pubmed
,
Echinobase
Nikitovic,
Effects of glycosaminoglycans on cell proliferation of normal osteoblasts and human osteosarcoma cells depend on their type and fine chemical compositions.
2005,
Pubmed
Nikolova,
Differential roles for membrane-bound and soluble syndecan-1 (CD138) in breast cancer progression.
2009,
Pubmed
O'Neill,
Structure and mechanics of starfish body wall.
1989,
Pubmed
,
Echinobase
Rouet,
A synthetic glycosaminoglycan mimetic binds vascular endothelial growth factor and modulates angiogenesis.
2005,
Pubmed
Sasisekharan,
Roles of heparan-sulphate glycosaminoglycans in cancer.
2002,
Pubmed
Smith,
Glycosaminoglycans as regulators of stem cell differentiation.
2011,
Pubmed
Staatz,
Analysis of proteoglycans and glycosaminoglycans from Drosophila.
2001,
Pubmed
Sugahara,
Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate.
2003,
Pubmed
Tovar,
The dermatan sulfate-dependent anticoagulant pathway is mostly preserved in aneurysm and in severe atherosclerotic lesions while the heparan sulfate pathway is disrupted.
2011,
Pubmed
Volpi,
Purification and characterization of hyaluronic acid from the mollusc bivalve Mytilus galloprovincialis.
2003,
Pubmed
Yamada,
Evolution of glycosaminoglycans: Comparative biochemical study.
2011,
Pubmed
Yip,
Therapeutic value of glycosaminoglycans in cancer.
2006,
Pubmed
Zou,
Chondroitin sulfate in palatal wound healing.
2004,
Pubmed