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ECB-ART-43398
J Biol Chem 2014 Jun 20;28925:17427-44. doi: 10.1074/jbc.M114.568949.
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Insights into the structure and function of ciliary and flagellar doublet microtubules: tektins, Ca2+-binding proteins, and stable protofilaments.

Linck R , Fu X , Lin J , Ouch C , Schefter A , Steffen W , Warren P , Nicastro D .


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Cilia and flagella are conserved, motile, and sensory cell organelles involved in signal transduction and human disease. Their scaffold consists of a 9-fold array of remarkably stable doublet microtubules (DMTs), along which motor proteins transmit force for ciliary motility and intraflagellar transport. DMTs possess Ribbons of three to four hyper-stable protofilaments whose location, organization, and specialized functions have been elusive. We performed a comprehensive analysis of the distribution and structural arrangements of Ribbon proteins from sea urchin sperm flagella, using quantitative immunobiochemistry, proteomics, immuno-cryo-electron microscopy, and tomography. Isolated Ribbons contain acetylated α-tubulin, β-tubulin, conserved protein Rib45, >95% of the axonemal tektins, and >95% of the calcium-binding proteins, Rib74 and Rib85.5, whose human homologues are related to the cause of juvenile myoclonic epilepsy. DMTs contain only one type of Ribbon, corresponding to protofilaments A11-12-13-1 of the A-tubule. Rib74 and Rib85.5 are associated with the Ribbon in the lumen of the A-tubule. Ribbons contain a single ∼5-nm wide filament, composed of equimolar tektins A, B, and C, which interact with the nexin-dynein regulatory complex. A summary of findings is presented, and the functions of Ribbon proteins are discussed in terms of the assembly and stability of DMTs, ciliary motility, and other microtubule systems.

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Genes referenced: dnah3 efhc1 ift52 LOC100887844 LOC100893907 LOC115919910 LOC591902 LOC594261 mapkap1 pam tubgcp2
???displayArticle.antibodies??? acetyl-LOC590586 Ab4 efhc1 Ab2 efhc2 Ab2 tekt Ab1 tekt1 Ab1 tekt2 Ab1 tekt4 Ab1


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References [+] :
Amos, The tektin family of microtubule-stabilizing proteins. 2008, Pubmed