Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
2014 Apr 29;11117:6317-22. doi: 10.1073/pnas.1400089111.
Show Gene links
Show Anatomy links
Sea star tenacity mediated by a protein that fragments, then aggregates.
Hennebert E
,
Wattiez R
,
Demeuldre M
,
Ladurner P
,
Hwang DS
,
Waite JH
,
Flammang P
.
???displayArticle.abstract???
Sea stars adhere firmly but temporarily to various substrata as a result of underwater efficient adhesive secretions released by their tube feet. Previous studies showed that this material is mainly made up of proteins, which play a key role in its adhesiveness and cohesiveness. Recently, we solubilized the majority of these proteins and obtained 43 de novo-generated peptide sequences by tandem MS. Here, one of these sequences served to recover the full-length sequence of Sea star footprint protein 1 (Sfp1), by RT-PCR and tube foot transcriptome analysis. Sfp1, a large protein of 3,853 aa, is the second most abundant constituent of the secreted adhesive. By using MS and Western blot analyses, we showed that Sfp1 is translated from a single mRNA and then cleaved into four subunits linked together by disulphide bridges in tube foot adhesive cells. The four subunits display specific protein-, carbohydrate-, and metal-binding domains. Immunohistochemistry and immunocytochemistry located Sfp1 in granules stockpiled by one of the two types of adhesive cells responsible for the secretion of the adhesive material. We also demonstrated that Sfp1 makes up the structural scaffold of the adhesive footprint that remains on the substratum after tube foot detachment. Taken together, the results suggest that Sfp1 is a major structural protein involved in footprint cohesion and possibly in adhesive interactions with the tube foot surface. In recombinant form, it could be used for the design of novel sea star-inspired biomaterials.
Aldred,
The adhesive strategies of cyprids and development of barnacle-resistant marine coatings.
2008, Pubmed
Aldred,
The adhesive strategies of cyprids and development of barnacle-resistant marine coatings.
2008,
Pubmed
Ameye,
Ultrastructure of the echinoderm cuticle after fast-freezing/freeze substitution and conventional chemical fixations.
2000,
Pubmed
,
Echinobase
Baumgartner,
The discoidin domain family revisited: new members from prokaryotes and a homology-based fold prediction.
1998,
Pubmed
Bi,
Processing, localization and binding activity of zonadhesin suggest a function in sperm adhesion to the zona pellucida during exocytosis of the acrosome.
2003,
Pubmed
Enghild,
Chondroitin 4-sulfate covalently cross-links the chains of the human blood protein pre-alpha-inhibitor.
1991,
Pubmed
Flammang,
The Role of Podial Secretions in Adhesion in Two Species of Sea Stars (Echinodermata).
1994,
Pubmed
,
Echinobase
Flammang,
A study of the temporary adhesion of the podia in the sea star asterias rubens (Echinodermata, asteroidea) through their footprints.
1998,
Pubmed
,
Echinobase
Gorb,
Biological attachment devices: exploring nature's diversity for biomimetics.
2008,
Pubmed
He,
Characterization of two 20kDa-cement protein (cp20k) homologues in Amphibalanus amphitrite.
2013,
Pubmed
Hennebert,
Characterization of the protein fraction of the temporary adhesive secreted by the tube feet of the sea star Asterias rubens.
2012,
Pubmed
,
Echinobase
Hennebert,
Characterisation of the carbohydrate fraction of the temporary adhesive secreted by the tube feet of the sea star Asterias rubens.
2011,
Pubmed
,
Echinobase
Hennebert,
Micro- and nanostructure of the adhesive material secreted by the tube feet of the sea star Asterias rubens.
2008,
Pubmed
,
Echinobase
Hwang,
Protein- and metal-dependent interactions of a prominent protein in mussel adhesive plaques.
2010,
Pubmed
Inoue,
Mussel adhesive plaque protein gene is a novel member of epidermal growth factor-like gene family.
1995,
Pubmed
Ishihama,
Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein.
2005,
Pubmed
Lee,
Mussel-Inspired Adhesives and Coatings.
2011,
Pubmed
Lee,
A reversible wet/dry adhesive inspired by mussels and geckos.
2007,
Pubmed
Lidell,
An autocatalytic cleavage in the C terminus of the human MUC2 mucin occurs at the low pH of the late secretory pathway.
2003,
Pubmed
Lidell,
Cleavage in the GDPH sequence of the C-terminal cysteine-rich part of the human MUC5AC mucin.
2006,
Pubmed
Marchler-Bauer,
CDD: a Conserved Domain Database for the functional annotation of proteins.
2011,
Pubmed
Mayadas,
Vicinal cysteines in the prosequence play a role in von Willebrand factor multimer assembly.
1992,
Pubmed
Meyer,
von Willebrand factor: structure and function.
1993,
Pubmed
Miseta,
Relationship between the occurrence of cysteine in proteins and the complexity of organisms.
2000,
Pubmed
Monnier,
RGM is a repulsive guidance molecule for retinal axons.
2002,
Pubmed
Nakano,
Self-assembling peptide inspired by a barnacle underwater adhesive protein.
2007,
Pubmed
Rao,
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions.
1995,
Pubmed
Santos,
Comparative histological and immunohistochemical study of sea star tube feet (Echinodermata, Asteroidea).
2005,
Pubmed
,
Echinobase
Silverman,
Understanding marine mussel adhesion.
2007,
Pubmed
Smith,
Differences in the Composition of Adhesive and Non-Adhesive Mucus From the Limpet Lottia limatula.
1999,
Pubmed
Sun,
Collagen-binding matrix proteins from elastomeric extraorganismic byssal fibers.
2002,
Pubmed
Tateno,
SUEL-related lectins, a lectin family widely distributed throughout organisms.
2010,
Pubmed
,
Echinobase
Taylor,
Chemoenzymatic synthesis of peptidyl 3,4-dihydroxyphenylalanine for structure-activity relationships in marine invertebrate polypeptides.
2002,
Pubmed
Thuveson,
The low pH in trans-Golgi triggers autocatalytic cleavage of pre-alpha -inhibitor heavy chain precursor.
2000,
Pubmed
Wang,
Localization of the bioadhesive precursors of the sandcastle worm, Phragmatopoma californica (Fewkes).
2012,
Pubmed
Whittington,
Adhesive secretions in the Platyhelminthes.
2001,
Pubmed
,
Echinobase
Zhang,
Interaction of hemojuvelin with neogenin results in iron accumulation in human embryonic kidney 293 cells.
2005,
Pubmed
