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In recent years, research on the autophagic process has greatly increased, invading the fields of biology and medicine. Several markers of the autophagic process have been discovered and various strategies have been reported studying this molecular process in different biological systems in both physiological and stress conditions. Furthermore, mechanisms of metalloid- or heavy metal-induced toxicity continue to be of interest given the ubiquitous nature and distribution of these contaminants in the environment where they often play the role of pollutants of numerous organisms. The aim of this review is a critical analysis and correlation of knowledge of autophagic mechanisms studied under stress for the most common arsenic (As) and cadmium (Cd) compounds. In this review we report data obtained in different experimental models for each compound, highlighting similarities and/or differences in the activation of autophagic processes. A more detailed discussion will concern the activation of autophagy in Cd-exposed sea urchin embryo since it is a suitable model system that is very sensitive to environmental stress, and Cd is one of the most studied heavy metal inductors of stress and modulator of different factors such as: protein kinase and phosphatase, caspases, mitochondria, heat shock proteins, metallothioneins, transcription factors, reactive oxygen species, apoptosis and autophagy.
Figure 1. Detection by immunofluorescence of LC3 protein on whole-mount embryos of sea urchin. Equatorial optical sections captured by confocal laser scanning microscopy. In green, LC3 protein detection; in red, nuclei stained with propidium iodide. (A) Control embryo, after 18 h of growth; (B) Cd-treated embryo for 18 h; (C) Control embryo, after 24 h of growth; (D) Cd-treated embryo for 24 h; (E, F) Enlargements of a section of (B, D), respectively. Bar = 50 μm.
Figure 2. Diagram showing an overview of the most representative cellular, biochemical, and molecular events activated as defense strategies in embryos and larvae of P.
lividus at different times post fertilization and various concentrations of CdCl2.
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