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Extremophiles
2013 Nov 01;176:1023-35. doi: 10.1007/s00792-013-0584-y.
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Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples.
Duarte AW
,
Dayo-Owoyemi I
,
Nobre FS
,
Pagnocca FC
,
Chaud LC
,
Pessoa A
,
Felipe MG
,
Sette LD
.
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The aim of the present study was to investigate the taxonomic identity of yeasts isolated from the Antarctic continent and to evaluate their ability to produce enzymes (lipase, protease and xylanase) at low and moderate temperatures. A total of 97 yeast strains were recovered from marine and terrestrial samples collected in the Antarctica. The highest amount of yeast strains was obtained from marine sediments, followed by lichens, ornithogenic soils, sea stars, Salpa sp., algae, sea urchin, sea squirt, stone with lichens, Nacella concinna, sea sponge, sea isopod and sea snail. Data from polyphasic taxonomy revealed the presence of 21 yeast species, distributed in the phylum Ascomycota (n = 8) and Basidiomycota (n = 13). Representatives of encapsulated yeasts, belonging to genera Rhodotorula and Cryptococcus were recovered from 7 different Antarctic samples. Moreover, Candida glaebosa, Cryptococcus victoriae, Meyerozyma (Pichia) guilliermondii, Rhodotorula mucilaginosa and R. laryngis were the most abundant yeast species recovered. This is the first report of the occurrence of some species of yeasts recovered from Antarctic marine invertebrates. Additionally, results from enzymes production at low/moderate temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which could be considered as a target for biotechnological applications. Among the evaluated yeasts in the present study 46.39, 37.11 and 14.43 % were able to produce lipase (at 15 °C), xylanase (at 15 °C) and protease (at 25 °C), respectively. The majority of lipolytic, proteolytic and xylanolytic strains were distributed in the phylum Basidiomycota and were mainly recovered from sea stars, lichens, sea urchin and marine sediments.
Burgaud,
Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna.
2010, Pubmed
Burgaud,
Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna.
2010,
Pubmed
Carrasco,
Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region.
2012,
Pubmed
Connell,
Diversity of soil yeasts isolated from South Victoria Land, Antarctica.
2008,
Pubmed
Crowe,
Anhydrobiosis.
1992,
Pubmed
Crowe,
Stabilization of dry phospholipid bilayers and proteins by sugars.
1987,
Pubmed
de Almeida,
Yeast community survey in the Tagus estuary.
2005,
Pubmed
Feller,
Psychrophilic enzymes: hot topics in cold adaptation.
2003,
Pubmed
Gadanho,
Cryptococcus ibericus sp. nov., Cryptococcus aciditolerans sp. nov. and Cryptococcus metallitolerans sp. nov., a new ecoclade of anamorphic basidiomycetous yeast species from an extreme environment associated with acid rock drainage in São Domingos pyrite mine, Portugal.
2009,
Pubmed
Gomes,
Thermolabile xylanase of the Antarctic yeast Cryptococcus adeliae: production and properties.
2000,
Pubmed
Joseph,
Cold active microbial lipases: some hot issues and recent developments.
2008,
Pubmed
Kouker,
Specific and sensitive plate assay for bacterial lipases.
1987,
Pubmed
Kutty,
Marine yeasts-a review.
2008,
Pubmed
Margesin,
Diversity and ecology of psychrophilic microorganisms.
2011,
Pubmed
Ohta,
Cell-associated beta-xylosidase from Aureobasidium pullulans ATCC 20524: Purification, properties, and characterization of the encoding gene.
2010,
Pubmed
Petrescu,
Xylanase from the psychrophilic yeast Cryptococcus adeliae.
2000,
Pubmed
Rao,
Purification and characterization of a novel aspartic protease from basidiomycetous yeast Cryptococcus sp. S-2.
2011,
Pubmed
Ray,
Extracellular protease from the antarctic yeast Candida humicola.
1992,
Pubmed
Richards,
Marine fungi: their ecology and molecular diversity.
2012,
Pubmed
Russell,
Cold adaptation of microorganisms.
1990,
Pubmed
Siddiqui,
Cold-adapted enzymes.
2006,
Pubmed
Subramaniyan,
Biotechnology of microbial xylanases: enzymology, molecular biology, and application.
2002,
Pubmed
Tamura,
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
2007,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Turchetti,
Psychrophilic yeasts in glacial environments of Alpine glaciers.
2008,
Pubmed
Turkiewicz,
A cold-adapted extracellular serine proteinase of the yeast Leucosporidium antarcticum.
2003,
Pubmed
Uetake,
Isolation of oligotrophic yeasts from supraglacial environments of different altitude on the Gulkana Glacier (Alaska).
2012,
Pubmed
Vaz,
The diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica.
2011,
Pubmed
Wang,
Purification and biochemical characterization of a cold-active lipase from Antarctic sea ice bacteria Pseudoalteromonas sp. NJ 70.
2012,
Pubmed
Yang,
Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site.
2002,
Pubmed
Yergeau,
Responses of Antarctic soil microbial communities and associated functions to temperature and freeze-thaw cycle frequency.
2008,
Pubmed
