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J Biol Chem
2013 Jun 28;28826:18825-33. doi: 10.1074/jbc.M113.451112.
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Polysialic acid is present in mammalian semen as a post-translational modification of the neural cell adhesion molecule NCAM and the polysialyltransferase ST8SiaII.
Simon P
,
Bäumner S
,
Busch O
,
Röhrich R
,
Kaese M
,
Richterich P
,
Wehrend A
,
Müller K
,
Gerardy-Schahn R
,
Mühlenhoff M
,
Geyer H
,
Geyer R
,
Middendorff R
,
Galuska SP
.
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Fertilization in animals is a complex sequence of several biochemical events beginning with the insemination into the female reproductive tract and, finally, leading to embryogenesis. Studies by Kitajima and co-workers (Miyata, S., Sato, C., and Kitajima, K. (2007) Trends Glycosci. Glyc, 19, 85-98) demonstrated the presence of polysialic acid (polySia) on sea urchin sperm. Based on these results, we became interested in the potential involvement of sialic acid polymers in mammalian fertilization. Therefore, we isolated human sperm and performed analyses, including Western blotting and mild 1,2-diamino-4,5-methylenedioxybenzene-HPLC, that revealed the presence α2,8-linked polySia chains. Further analysis by a glyco-proteomics approach led to the identification of two polySia carriers. Interestingly, besides the neural cell adhesion molecule, the polysialyltransferase ST8SiaII has also been found to be a target for polysialylation. Further analysis of testis and epididymis tissue sections demonstrated that only epithelial cells of the caput were polySia-positive. During the epididymal transit, polySia carriers were partially integrated into the sperm membrane of the postacrosomal region. Because polySia is known to counteract histone as well as neutrophil extracellular trap-mediated cytotoxicity against host cells, which plays a role after insemination, we propose that polySia in semen represents a cytoprotective element to increase the number of vital sperm.
Aitken,
Proteomic changes in mammalian spermatozoa during epididymal maturation.
2007, Pubmed
Aitken,
Proteomic changes in mammalian spermatozoa during epididymal maturation.
2007,
Pubmed
Alghamdi,
Seminal DNase frees spermatozoa entangled in neutrophil extracellular traps.
2005,
Pubmed
Angata,
Differential and cooperative polysialylation of the neural cell adhesion molecule by two polysialyltransferases, PST and STX.
1998,
Pubmed
Bax,
Interaction of polysialic acid with CCL21 regulates the migratory capacity of human dendritic cells.
2009,
Pubmed
Bellin,
Fertility-associated antigen on bull sperm indicates fertility potential.
1998,
Pubmed
Brinkmann,
Neutrophil extracellular traps kill bacteria.
2004,
Pubmed
Brinkmann,
Beneficial suicide: why neutrophils die to make NETs.
2007,
Pubmed
Brusés,
Roles, regulation, and mechanism of polysialic acid function during neural development.
2001,
Pubmed
Close,
Polysialyltransferase-1 autopolysialylation is not requisite for polysialylation of neural cell adhesion molecule.
2000,
Pubmed
Close,
In vivo autopolysialylation and localization of the polysialyltransferases PST and STX.
1998,
Pubmed
Close,
The polysialyltransferase ST8Sia II/STX: posttranslational processing and role of autopolysialylation in the polysialylation of neural cell adhesion molecule.
2001,
Pubmed
Close,
The minimal structural domains required for neural cell adhesion molecule polysialylation by PST/ST8Sia IV and STX/ST8Sia II.
2003,
Pubmed
Curreli,
Polysialylated neuropilin-2 is expressed on the surface of human dendritic cells and modulates dendritic cell-T lymphocyte interactions.
2007,
Pubmed
Drake,
Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses.
2008,
Pubmed
Drake,
Polysialic acid governs T-cell development by regulating progenitor access to the thymus.
2009,
Pubmed
Drobnis,
Natural history of mammalian spermatozoa in the female reproductive tract.
1992,
Pubmed
Finne,
Occurrence of unique polysialosyl carbohydrate units in glycoproteins of developing brain.
1982,
Pubmed
Frosch,
NZB mouse system for production of monoclonal antibodies to weak bacterial antigens: isolation of an IgG antibody to the polysaccharide capsules of Escherichia coli K1 and group B meningococci.
1985,
Pubmed
Fujimoto,
Regulation of cell adhesion by polysialic acid. Effects on cadherin, immunoglobulin cell adhesion molecule, and integrin function and independence from neural cell adhesion molecule binding or signaling activity.
2001,
Pubmed
Galuska,
Synaptic cell adhesion molecule SynCAM 1 is a target for polysialylation in postnatal mouse brain.
2010,
Pubmed
Galuska,
Glycomic strategy for efficient linkage analysis of di-, oligo- and polysialic acids.
2012,
Pubmed
Galuska,
Polysialic acid profiles of mice expressing variant allelic combinations of the polysialyltransferases ST8SiaII and ST8SiaIV.
2006,
Pubmed
Galuska,
Enzyme-dependent variations in the polysialylation of the neural cell adhesion molecule (NCAM) in vivo.
2008,
Pubmed
Gatti,
Post-testicular sperm environment and fertility.
2004,
Pubmed
Gerardy-Schahn,
Topography of NCAM antigenic epitopes recognized by SCLC-cluster-1 antibodies. A consensus view.
1994,
Pubmed
Haselhorst,
Endosialidase NF appears to bind polySia DP5 in a helical conformation.
2006,
Pubmed
Inoue,
Chemical analysis of the developmental pattern of polysialylation in chicken brain. Expression of only an extended form of polysialyl chains during embryogenesis and the presence of disialyl residues in both embryonic and adult chicken brains.
2000,
Pubmed
Inoue,
Developmental profile of neural cell adhesion molecule glycoforms with a varying degree of polymerization of polysialic acid chains.
2001,
Pubmed
Inoue,
An ultrasensitive chemical method for polysialic acid analysis.
2001,
Pubmed
Inoue,
A challenge to the ultrasensitive chemical method for the analysis of oligo- and polysialic acids at a nanogram level of colominic acid and a milligram level of brain tissues.
2001,
Pubmed
Lackie,
Polysialic acid and N-CAM localisation in embryonic rat kidney: mesenchymal and epithelial elements show different patterns of expression.
1990,
Pubmed
Lackie,
Expression of polysialylated N-CAM during rat heart development.
1991,
Pubmed
Lackie,
Polysialic acid of the neural cell adhesion molecule (N-CAM) is widely expressed during organogenesis in mesodermal and endodermal derivatives.
1994,
Pubmed
Liedtke,
Characterization of N-glycans from mouse brain neural cell adhesion molecule.
2001,
Pubmed
Marengo,
Maturing the sperm: unique mechanisms for modifying integral proteins in the sperm plasma membrane.
2008,
Pubmed
McCauley,
Purification and characterization of fertility-associated antigen (FAA) in bovine seminal fluid.
1999,
Pubmed
Miyata,
Flagellasialin: a novel sulfated alpha2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella.
2006,
Pubmed
,
Echinobase
Moolenaar,
Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212.
1990,
Pubmed
Mühlenhoff,
The impact of N-glycosylation on the functions of polysialyltransferases.
2001,
Pubmed
Mühlenhoff,
Autocatalytic polysialylation of polysialyltransferase-1.
1996,
Pubmed
Nakata,
Degree of polymerization (DP) of polysialic acid (polySia) on neural cell adhesion molecules (N-CAMS): development and application of a new strategy to accurately determine the DP of polySia chains on N-CAMS.
2005,
Pubmed
Nelson,
Protein determinants for specific polysialylation of the neural cell adhesion molecule.
1995,
Pubmed
Pandya,
The leukocytic reaction of the human uterine cervix to spermatozoa.
1985,
Pubmed
Pang,
Expression of bisecting type and Lewisx/Lewisy terminated N-glycans on human sperm.
2007,
Pubmed
Rey-Gallardo,
Polysialic acid is required for neuropilin-2a/b-mediated control of CCL21-driven chemotaxis of mature dendritic cells and for their migration in vivo.
2011,
Pubmed
Roepstorff,
Proposal for a common nomenclature for sequence ions in mass spectra of peptides.
1984,
Pubmed
Rolland,
Decline in semen concentration and morphology in a sample of 26,609 men close to general population between 1989 and 2005 in France.
2013,
Pubmed
Rutishauser,
Polysialic acid in the plasticity of the developing and adult vertebrate nervous system.
2008,
Pubmed
Rutishauser,
NCAM and its polysialic acid moiety: a mechanism for pull/push regulation of cell interactions during development?
1992,
Pubmed
Rutishauser,
Polysialic acid and the regulation of cell interactions.
1996,
Pubmed
Saez,
Epididymosomes and prostasomes: their roles in posttesticular maturation of the sperm cells.
2003,
Pubmed
Saffarzadeh,
Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones.
2012,
Pubmed
Saffarzadeh,
Fighting against the dark side of neutrophil extracellular traps in disease: manoeuvres for host protection.
2013,
Pubmed
Sato,
Development of a highly sensitive chemical method for detecting alpha2-->8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane.
1998,
Pubmed
Stummeyer,
Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F.
2005,
Pubmed
Sullivan,
Epididymosomes are involved in the acquisition of new sperm proteins during epididymal transit.
2007,
Pubmed
Swan,
The question of declining sperm density revisited: an analysis of 101 studies published 1934-1996.
2000,
Pubmed
Tang,
Polysialic acid influences specific pathfinding by avian motoneurons.
1992,
Pubmed
Thimon,
Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach.
2008,
Pubmed
Thompson,
The leukocytic reaction of the human uterine cervix.
1992,
Pubmed
Troy,
Polysialylation: from bacteria to brains.
1992,
Pubmed
Ulm,
Soluble polysialylated NCAM: a novel player of the innate immune system in the lung.
2013,
Pubmed
Walsh,
Neural cell adhesion molecules of the immunoglobulin superfamily: role in axon growth and guidance.
1997,
Pubmed
Wang,
Alleviation of neurotoxicity by microglial human Siglec-11.
2010,
Pubmed
Wang,
Expression of Siglec-11 by human and chimpanzee ovarian stromal cells, with uniquely human ligands: implications for human ovarian physiology and pathology.
2011,
Pubmed
Weinhold,
Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule.
2005,
Pubmed
Yabe,
Polysialic acid in human milk. CD36 is a new member of mammalian polysialic acid-containing glycoprotein.
2003,
Pubmed
Zuber,
Polysialic acid is associated with sodium channels and the neural cell adhesion molecule N-CAM in adult rat brain.
1992,
Pubmed
von Der Ohe,
Localization and characterization of polysialic acid-containing N-linked glycans from bovine NCAM.
2002,
Pubmed