Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-42751
Cell Rep 2013 Feb 21;32:309-18. doi: 10.1016/j.celrep.2013.01.008.
Show Gene links Show Anatomy links

Genome-wide association of Yorkie with chromatin and chromatin-remodeling complexes.

Oh H , Slattery M , Ma L , Crofts A , White KP , Mann RS , Irvine KD .


???displayArticle.abstract???
The Hippo pathway regulates growth through the transcriptional coactivator Yorkie, but how Yorkie promotes transcription remains poorly understood. We address this by characterizing Yorkie''s association with chromatin and by identifying nuclear partners that effect transcriptional activation. Coimmunoprecipitation and mass spectrometry identify GAGA factor (GAF), the Brahma complex, and the Mediator complex as Yorkie-associated nuclear protein complexes. All three are required for Yorkie''s transcriptional activation of downstream genes, and GAF and the Brahma complex subunit Moira interact directly with Yorkie. Genome-wide chromatin-binding experiments identify thousands of Yorkie sites, most of which are associated with elevated transcription, based on genome-wide analysis of messenger RNA and histone H3K4Me3 modification. Chromatin binding also supports extensive functional overlap between Yorkie and GAF. Our studies suggest a widespread role for Yorkie as a regulator of transcription and identify recruitment of the chromatin-modifying GAF protein and BRM complex as a molecular mechanism for transcriptional activation by Yorkie.

???displayArticle.pubmedLink??? 23395637
???displayArticle.pmcLink??? PMC3633442
???displayArticle.link??? Cell Rep
???displayArticle.grants??? [+]


References [+] :
Adkins, GAGA protein: a multi-faceted transcription factor. 2006, Pubmed