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Cell Calcium
2012 Jan 01;523-4:217-25. doi: 10.1016/j.ceca.2012.06.009.
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Observations of calcium dynamics in cortical secretory vesicles.
Raveh A
,
Valitsky M
,
Shani L
,
Coorssen JR
,
Blank PS
,
Zimmerberg J
,
Rahamimoff R
.
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Calcium (Ca(2+)) dynamics were evaluated in fluorescently labeled sea urchin secretory vesicles using confocal microscopy. 71% of the vesicles examined exhibited one or more transient increases in the fluorescence signal that was damped in time. The detection of transient increases in signal was dependent upon the affinity of the fluorescence indicator; the free Ca(2+) concentration in the secretory vesicles was estimated to be in the range of ∼10 to 100 μM. Non-linear stochastic analysis revealed the presence of extra variance in the Ca(2+) dependent fluorescence signal. This noise process increased linearly with the amplitude of the Ca(2+) signal. Both the magnitude and spatial properties of this noise process were dependent upon the activity of vesicle p-type (Ca(v)2.1) Ca(2+) channels. Blocking the p-type Ca(2+) channels with ω-agatoxin decreased signal variance, and altered the spatial noise pattern within the vesicle. These fluorescence signal properties are consistent with vesicle Ca(2+) dynamics and not simply due to obvious physical properties such as gross movement artifacts or pH driven changes in Ca(2+) indicator fluorescence. The results suggest that the free Ca(2+) content of cortical secretory vesicles is dynamic; this property may modulate the exocytotic fusion process.
Allbritton,
Localized calcium spikes and propagating calcium waves.
1993, Pubmed
Allbritton,
Localized calcium spikes and propagating calcium waves.
1993,
Pubmed
Berridge,
Calcium signalling: dynamics, homeostasis and remodelling.
2003,
Pubmed
Berridge,
The versatility and universality of calcium signalling.
2000,
Pubmed
Blank,
Submaximal responses in calcium-triggered exocytosis are explained by differences in the calcium sensitivity of individual secretory vesicles.
1998,
Pubmed
,
Echinobase
Blinova,
Fluctuation analysis of mitochondrial NADH fluorescence signals in confocal and two-photon microscopy images of living cardiac myocytes.
2004,
Pubmed
Bootman,
The organisation and functions of local Ca(2+) signals.
2001,
Pubmed
Bootman,
Calcium signalling--an overview.
2001,
Pubmed
Budd,
A reevaluation of the role of mitochondria in neuronal Ca2+ homeostasis.
1996,
Pubmed
Christensen,
pH-dependent regulation of lysosomal calcium in macrophages.
2002,
Pubmed
Davis,
Ca(2+) signaling occurs via second messenger release from intraorganelle synthesis sites.
2008,
Pubmed
,
Echinobase
Degtyar,
Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.
2007,
Pubmed
Demaurex,
pH Homeostasis of cellular organelles.
2002,
Pubmed
Eberhard,
Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescence.
1989,
Pubmed
Falcke,
Introduction to focus issue: intracellular Ca2+ dynamics--a change of modeling paradigm?
2009,
Pubmed
Ferrell,
Tripping the switch fantastic: how a protein kinase cascade can convert graded inputs into switch-like outputs.
1996,
Pubmed
Fossier,
Control of the calcium concentration involved in acetylcholine release and its facilitation: an additional role for synaptic vesicles?
1998,
Pubmed
Gillot,
The calcium content of cortical granules and the loss of calcium from sea urchin eggs at fertilization.
1991,
Pubmed
,
Echinobase
Haynes,
Vesicular Ca(2+) -induced secretion promoted by intracellular pH-gradient disruption.
2006,
Pubmed
Kelly,
Storage and release of neurotransmitters.
1993,
Pubmed
Knight,
Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death.
2003,
Pubmed
Kobayashi,
Photon statistics and correlation analysis of ultraweak light originating from living organisms for extraction of biological information.
2000,
Pubmed
Lattanzio,
The effects of pH and temperature on fluorescent calcium indicators as determined with Chelex-100 and EDTA buffer systems.
1990,
Pubmed
Lattanzio,
The effect of pH on rate constants, ion selectivity and thermodynamic properties of fluorescent calcium and magnesium indicators.
1991,
Pubmed
Lidke,
The role of photon statistics in fluorescence anisotropy imaging.
2005,
Pubmed
Lii,
Characterization of a large conductance, cation-selective channel from sea urchin eggs that is sensitive to sulfhydryl reducing agents.
1996,
Pubmed
,
Echinobase
Machado,
On the role of intravesicular calcium in the motion and exocytosis of secretory organelles.
2009,
Pubmed
Mahapatra,
A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein.
2004,
Pubmed
Malgaroli,
Spontaneous [Ca2+]i fluctuations in rat chromaffin cells do not require inositol 1,4,5-trisphosphate elevations but are generated by a caffeine- and ryanodine-sensitive intracellular Ca2+ store.
1990,
Pubmed
Meir,
Ion channels in presynaptic nerve terminals and control of transmitter release.
1999,
Pubmed
Melamed,
Confocal microscopy reveals coordinated calcium fluctuations and oscillations in synaptic boutons.
1993,
Pubmed
Melamed-Book,
Neuronal calcium sparks and intracellular calcium "noise".
1999,
Pubmed
Minta,
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores.
1989,
Pubmed
Mitchell,
Ryanodine receptor type I and nicotinic acid adenine dinucleotide phosphate receptors mediate Ca2+ release from insulin-containing vesicles in living pancreatic beta-cells (MIN6).
2003,
Pubmed
Mitchell,
Dense core secretory vesicles revealed as a dynamic Ca(2+) store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera.
2001,
Pubmed
Moreno,
Calcium dynamics in catecholamine-containing secretory vesicles.
2005,
Pubmed
Mundorf,
Vesicular Ca(2+) participates in the catalysis of exocytosis.
2000,
Pubmed
Nguyen,
Role of Ca2+/K+ ion exchange in intracellular storage and release of Ca2+.
1998,
Pubmed
Oberdorf,
Calcium uptake and release by isolated cortices and microsomes from the unfertilized egg of the sea urchin Strongylocentrotus droebachiensis.
1986,
Pubmed
,
Echinobase
Ozbudak,
Regulation of noise in the expression of a single gene.
2002,
Pubmed
Parekh,
Store depletion and calcium influx.
1997,
Pubmed
Payan,
Uptake and release of calcium by isolated egg cortices of the sea urchin Paracentrotus lividus.
1986,
Pubmed
,
Echinobase
Petersen,
Calcium signalling: past, present and future.
2005,
Pubmed
Petersen,
Polarized calcium signaling in exocrine gland cells.
2008,
Pubmed
Quesada,
InsP3 signaling induces pulse-modulated Ca2+ signals in the nucleus of airway epithelial ciliated cells.
2005,
Pubmed
Quesada,
ATP-independent luminal oscillations and release of Ca2+ and H+ from mast cell secretory granules: implications for signal transduction.
2003,
Pubmed
Quesada,
Mouse mast cell secretory granules can function as intracellular ionic oscillators.
2001,
Pubmed
Rahamimoff,
Ion channels in synaptic vesicles from Torpedo electric organ.
1988,
Pubmed
Sammels,
Intracellular Ca2+ storage in health and disease: a dynamic equilibrium.
2010,
Pubmed
Santodomingo,
Calcium dynamics in bovine adrenal medulla chromaffin cell secretory granules.
2008,
Pubmed
Sardet,
The ultrastructure of the sea urchin egg cortex isolated before and after fertilization.
1984,
Pubmed
,
Echinobase
Sarvazyan,
Effects of oxidants on properties of fluorescent calcium indicators.
1998,
Pubmed
Scheenen,
Ca2+ depletion from granules inhibits exocytosis. A study with insulin-secreting cells.
1998,
Pubmed
Scheenen,
Photodegradation of indo-1 and its effect on apparent Ca2+ concentrations.
1996,
Pubmed
Schmidt,
Metal ion content of cholinergic synaptic vesicles isolated from the electric organ of Torpedo: effect of stimulation-induced transmitter release.
1980,
Pubmed
Schneggenburger,
Presynaptic calcium and control of vesicle fusion.
2005,
Pubmed
Skupin,
Statistical properties and information content of calcium oscillations.
2007,
Pubmed
Skupin,
From puffs to global Ca2+ signals: how molecular properties shape global signals.
2009,
Pubmed
Smith,
Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.
2000,
Pubmed
,
Echinobase
Stanley,
Evidence for anion channels in secretory vesicles.
1988,
Pubmed
Tabb,
Glutamate transport into synaptic vesicles. Roles of membrane potential, pH gradient, and intravesicular pH.
1992,
Pubmed
Thattai,
Attenuation of noise in ultrasensitive signaling cascades.
2002,
Pubmed
Thattai,
Intrinsic noise in gene regulatory networks.
2001,
Pubmed
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Vogel,
Calcium influx is required for endocytotic membrane retrieval.
1999,
Pubmed
,
Echinobase
Yakir,
The non-specific ion channel in Torpedo ocellata fused synaptic vesicles.
1995,
Pubmed
Zimmerberg,
Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.
1999,
Pubmed
,
Echinobase
Ziv,
Optimal signal processing in small stochastic biochemical networks.
2007,
Pubmed
