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Cell Rep
2012 Jun 28;16:632-8. doi: 10.1016/j.celrep.2012.04.011.
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Voltage-dependent calcium channels at the plasma membrane, but not vesicular channels, couple exocytosis to endocytosis.
Xue L
,
Zhang Z
,
McNeil BD
,
Luo F
,
Wu XS
,
Sheng J
,
Shin W
,
Wu LG
.
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Although calcium influx triggers endocytosis at many synapses and non-neuronal secretory cells, the identity of the calcium channel is unclear. The plasma membrane voltage-dependent calcium channel (VDCC) is a candidate, and it was recently proposed that exocytosis transiently inserts vesicular calcium channels at the plasma membrane, thus triggering endocytosis and coupling it to exocytosis, a mechanism suggested to be conserved from sea urchin to human. Here, we report that the vesicular membrane, when inserted into the plasma membrane upon exocytosis, does not generate a calcium current or calcium increase at a mammalian nerve terminal. Instead, VDCCs at the plasma membrane, including the P/Q-type, provide the calcium influx to trigger rapid and slow endocytosis and, thus, couple endocytosis to exocytosis. These findings call for reconsideration of the vesicular calcium channel hypothesis. They are likely to apply to many synapses and non-neuronal cells in which VDCCs control exocytosis, and exocytosis is coupled to endocytosis.
Artalejo,
Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin.
1995, Pubmed
Artalejo,
Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin.
1995,
Pubmed
Balaji,
Calcium control of endocytic capacity at a CNS synapse.
2008,
Pubmed
Beutner,
Calcium dependence of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse.
2001,
Pubmed
Brose,
Flowers for synaptic endocytosis.
2009,
Pubmed
Ceccarelli,
Ca2+-dependent recycling of synaptic vesicles at the frog neuromuscular junction.
1980,
Pubmed
Clayton,
Activity-dependent control of bulk endocytosis by protein dephosphorylation in central nerve terminals.
2007,
Pubmed
Cousin,
Ba2+ does not support synaptic vesicle retrieval in rat cerebrocortical synaptosomes.
1998,
Pubmed
Forsythe,
Inactivation of presynaptic calcium current contributes to synaptic depression at a fast central synapse.
1998,
Pubmed
Gad,
Dissociation between Ca2+-triggered synaptic vesicle exocytosis and clathrin-mediated endocytosis at a central synapse.
1998,
Pubmed
He,
Two modes of fusion pore opening revealed by cell-attached recordings at a synapse.
2006,
Pubmed
He,
Ca2+ triggers a novel clathrin-independent but actin-dependent fast endocytosis in pancreatic beta cells.
2008,
Pubmed
Hosoi,
Calcium dependence of exo- and endocytotic coupling at a glutamatergic synapse.
2009,
Pubmed
Iwasaki,
Developmental changes in calcium channel types mediating central synaptic transmission.
2000,
Pubmed
Kuo,
A new ion channel blooms at the synapse.
2009,
Pubmed
Leitz,
Ca²⁺ influx slows single synaptic vesicle endocytosis.
2011,
Pubmed
Li,
Differential gating and recruitment of P/Q-, N-, and R-type Ca2+ channels in hippocampal mossy fiber boutons.
2007,
Pubmed
Marks,
Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals.
1998,
Pubmed
Neves,
Calcium influx selects the fast mode of endocytosis in the synaptic terminal of retinal bipolar cells.
2001,
Pubmed
Poskanzer,
Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo.
2003,
Pubmed
Royle,
Endocytosis at the synaptic terminal.
2003,
Pubmed
Sankaranarayanan,
Calcium accelerates endocytosis of vSNAREs at hippocampal synapses.
2001,
Pubmed
Sankaranarayanan,
Real-time measurements of vesicle-SNARE recycling in synapses of the central nervous system.
2000,
Pubmed
Schneggenburger,
The calyx of Held.
2006,
Pubmed
Schweizer,
The synaptic vesicle: cycle of exocytosis and endocytosis.
2006,
Pubmed
Shupliakov,
Recent insights into the building and cycling of synaptic vesicles.
2010,
Pubmed
Smith,
Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.
2000,
Pubmed
,
Echinobase
Sun,
Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse.
2002,
Pubmed
Sun,
Capacitance measurements at the calyx of Held in the medial nucleus of the trapezoid body.
2004,
Pubmed
Sun,
Fast kinetics of exocytosis revealed by simultaneous measurements of presynaptic capacitance and postsynaptic currents at a central synapse.
2001,
Pubmed
Vogel,
Channeling calcium: a shared mechanism for exocytosis-endocytosis coupling.
2009,
Pubmed
,
Echinobase
von Gersdorff,
Inhibition of endocytosis by elevated internal calcium in a synaptic terminal.
1994,
Pubmed
Weber,
N-type Ca2+ channels carry the largest current: implications for nanodomains and transmitter release.
2010,
Pubmed
Wu,
Rapid endocytosis does not recycle vesicles within the readily releasable pool.
2009,
Pubmed
Wu,
Ca(2+) and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal.
2009,
Pubmed
Wu,
Calcium channel types with distinct presynaptic localization couple differentially to transmitter release in single calyx-type synapses.
1999,
Pubmed
Wu,
Modes of vesicle retrieval at ribbon synapses, calyx-type synapses, and small central synapses.
2007,
Pubmed
Wu,
Activity-dependent acceleration of endocytosis at a central synapse.
2005,
Pubmed
Xu,
Role of Ca(2+) channels in short-term synaptic plasticity.
2007,
Pubmed
Xu,
The decrease in the presynaptic calcium current is a major cause of short-term depression at a calyx-type synapse.
2005,
Pubmed
Yamashita,
Developmental shift to a mechanism of synaptic vesicle endocytosis requiring nanodomain Ca2+.
2010,
Pubmed
Yao,
A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis.
2009,
Pubmed
