Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Biol
2012 Jun 15;3662:298-307. doi: 10.1016/j.ydbio.2012.03.015.
Show Gene links
Show Anatomy links
The Nkx5/HMX homeodomain protein MLS-2 is required for proper tube cell shape in the C. elegans excretory system.
Abdus-Saboor I
,
Stone CE
,
Murray JI
,
Sundaram MV
.
???displayArticle.abstract???
Cells perform wide varieties of functions that are facilitated, in part, by adopting unique shapes. Many of the genes and pathways that promote cell fate specification have been elucidated. However, relatively few transcription factors have been identified that promote shape acquisition after fate specification. Here we show that the Nkx5/HMX homeodomain protein MLS-2 is required for cellular elongation and shape maintenance of two tubular epithelial cells in the C. elegans excretory system, the duct and pore cells. The Nkx5/HMX family is highly conserved from sea urchins to humans, with known roles in neuronal and glial development. MLS-2 is expressed in the duct and pore, and defects in mls-2 mutants first arise when the duct and pore normally adopt unique shapes. MLS-2 cooperates with the EGF-Ras-ERK pathway to turn on the LIN-48/Ovo transcription factor in the duct cell during morphogenesis. These results reveal a novel interaction between the Nkx5/HMX family and the EGF-Ras pathway and implicate a transcription factor, MLS-2, as a regulator of cell shape.
Abdus-Saboor,
Notch and Ras promote sequential steps of excretory tube development in C. elegans.
2011, Pubmed
Abdus-Saboor,
Notch and Ras promote sequential steps of excretory tube development in C. elegans.
2011,
Pubmed
Andrew,
Morphogenesis of epithelial tubes: Insights into tube formation, elongation, and elaboration.
2010,
Pubmed
Bao,
Automated cell lineage tracing in Caenorhabditis elegans.
2006,
Pubmed
Bargmann,
Odorant-selective genes and neurons mediate olfaction in C. elegans.
1993,
Pubmed
Beitel,
The Caenorhabditis elegans gene lin-1 encodes an ETS-domain protein and defines a branch of the vulval induction pathway.
1995,
Pubmed
Bobinnec,
Identification and characterization of Caenorhabditis elegans gamma-tubulin in dividing cells and differentiated tissues.
2000,
Pubmed
Boyle,
AceTree: a tool for visual analysis of Caenorhabditis elegans embryogenesis.
2006,
Pubmed
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
Buechner,
Tubes and the single C. elegans excretory cell.
2002,
Pubmed
Cartier,
Pax6-induced alteration of cell fate: shape changes, expression of neuronal alpha tubulin, postmitotic phenotype, and cell migration.
2006,
Pubmed
Chalfie,
Structural and functional diversity in the neuronal microtubules of Caenorhabditis elegans.
1982,
Pubmed
Chamberlin,
Characterization of seven genes affecting Caenorhabditis elegans hindgut development.
1999,
Pubmed
Chang,
Intermediate filaments mediate cytoskeletal crosstalk.
2004,
Pubmed
Chanut-Delalande,
Shavenbaby couples patterning to epidermal cell shape control.
2006,
Pubmed
Chesarone,
Unleashing formins to remodel the actin and microtubule cytoskeletons.
2010,
Pubmed
Dai,
The ovo gene required for cuticle formation and oogenesis in flies is involved in hair formation and spermatogenesis in mice.
1998,
Pubmed
Deitcher,
Asymmetric expression of a novel homeobox gene in vertebrate sensory organs.
1994,
Pubmed
Frankel,
Morphological evolution caused by many subtle-effect substitutions in regulatory DNA.
2011,
Pubmed
Frankel,
Phenotypic robustness conferred by apparently redundant transcriptional enhancers.
2010,
Pubmed
Friedman,
The Foxa family of transcription factors in development and metabolism.
2006,
Pubmed
Fuchs,
Bridging cytoskeletal intersections.
2001,
Pubmed
Fukushige,
Selective expression of the tba-1 alpha tubulin gene in a set of mechanosensory and motor neurons during the development of Caenorhabditis elegans.
1995,
Pubmed
Gehring,
Pax 6: mastering eye morphogenesis and eye evolution.
1999,
Pubmed
Goldman,
The function of intermediate filaments in cell shape and cytoskeletal integrity.
1996,
Pubmed
Gongal,
Hmx4 regulates Sonic hedgehog signaling through control of retinoic acid synthesis during forebrain patterning.
2011,
Pubmed
Heiman,
DEX-1 and DYF-7 establish sensory dendrite length by anchoring dendritic tips during cell migration.
2009,
Pubmed
Herrmann,
Intermediate filaments: from cell architecture to nanomechanics.
2007,
Pubmed
Howard,
C. elegans EOR-1/PLZF and EOR-2 positively regulate Ras and Wnt signaling and function redundantly with LIN-25 and the SUR-2 Mediator component.
2002,
Pubmed
Hurd,
Specific alpha- and beta-tubulin isotypes optimize the functions of sensory Cilia in Caenorhabditis elegans.
2010,
Pubmed
Jiang,
The HMX homeodomain protein MLS-2 regulates cleavage orientation, cell proliferation and cell fate specification in the C. elegans postembryonic mesoderm.
2005,
Pubmed
Johnson,
EGL-38 Pax regulates the ovo-related gene lin-48 during Caenorhabditis elegans organ development.
2001,
Pubmed
Kamei,
Endothelial tubes assemble from intracellular vacuoles in vivo.
2006,
Pubmed
Kanning,
Motor neuron diversity in development and disease.
2010,
Pubmed
Kim,
The HMX/NKX homeodomain protein MLS-2 specifies the identity of the AWC sensory neuron type via regulation of the ceh-36 Otx gene in C. elegans.
2010,
Pubmed
Koh,
ELT-5 and ELT-6 are required continuously to regulate epidermal seam cell differentiation and cell fusion in C. elegans.
2001,
Pubmed
Köppen,
Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia.
2001,
Pubmed
Lackner,
Genetic analysis of the Caenorhabditis elegans MAP kinase gene mpk-1.
1998,
Pubmed
Lanjuin,
Otx/otd homeobox genes specify distinct sensory neuron identities in C. elegans.
2003,
Pubmed
Lehner,
Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways.
2006,
Pubmed
Leung,
Plakins: a family of versatile cytolinker proteins.
2002,
Pubmed
Lloyd,
Microtubules and the shape of plants to come.
2004,
Pubmed
Lubarsky,
Tube morphogenesis: making and shaping biological tubes.
2003,
Pubmed
Mancuso,
Extracellular leucine-rich repeat proteins are required to organize the apical extracellular matrix and maintain epithelial junction integrity in C. elegans.
2012,
Pubmed
Mango,
The molecular basis of organ formation: insights from the C. elegans foregut.
2009,
Pubmed
Martinez,
SpHmx, a sea urchin homeobox gene expressed in embryonic pigment cells.
1997,
Pubmed
,
Echinobase
Marín,
Neurons in motion: same principles for different shapes?
2006,
Pubmed
Mason,
The extending astroglial process: development of glial cell shape, the growing tip, and interactions with neurons.
1988,
Pubmed
Mattingly,
The FGD homologue EXC-5 regulates apical trafficking in C. elegans tubules.
2011,
Pubmed
McKean,
The extended tubulin superfamily.
2001,
Pubmed
McLean,
A novel peripherin isoform generated by alternative translation is required for normal filament network formation.
2008,
Pubmed
Meinertzhagen,
From form to function: the ways to know a neuron.
2009,
Pubmed
Melki,
Cold depolymerization of microtubules to double rings: geometric stabilization of assemblies.
1989,
Pubmed
Murray,
The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree.
2006,
Pubmed
Nelson,
Fine structure of the Caenorhabditis elegans secretory-excretory system.
1983,
Pubmed
Ohmachi,
C. elegans ksr-1 and ksr-2 have both unique and redundant functions and are required for MPK-1 ERK phosphorylation.
2002,
Pubmed
Oikonomou,
The glia of Caenorhabditis elegans.
2011,
Pubmed
Otani,
IKKε regulates cell elongation through recycling endosome shuttling.
2011,
Pubmed
Payre,
ovo/svb integrates Wingless and DER pathways to control epidermis differentiation.
1999,
Pubmed
Peinado,
Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype?
2007,
Pubmed
Pollard,
Actin, a central player in cell shape and movement.
2009,
Pubmed
Qian,
Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across species.
2011,
Pubmed
Rasmussen,
Notch signaling and morphogenesis of single-cell tubes in the C. elegans digestive tract.
2008,
Pubmed
Rocheleau,
The Caenorhabditis elegans ekl (enhancer of ksr-1 lethality) genes include putative components of a germline small RNA pathway.
2008,
Pubmed
Rocheleau,
A lin-45 raf enhancer screen identifies eor-1, eor-2 and unusual alleles of Ras pathway genes in Caenorhabditis elegans.
2002,
Pubmed
Santella,
A hybrid blob-slice model for accurate and efficient detection of fluorescence labeled nuclei in 3D.
2010,
Pubmed
Schottenfeld,
Tube continued: morphogenesis of the Drosophila tracheal system.
2010,
Pubmed
Singh,
sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction.
1995,
Pubmed
Smith,
Stretch growth of integrated axon tracts: extremes and exploitations.
2009,
Pubmed
Stiess,
Neuronal polarization: the cytoskeleton leads the way.
2011,
Pubmed
Stone,
Lipocalin signaling controls unicellular tube development in the Caenorhabditis elegans excretory system.
2009,
Pubmed
Sulston,
The embryonic cell lineage of the nematode Caenorhabditis elegans.
1983,
Pubmed
Tuck,
lin-25, a gene required for vulval induction in Caenorhabditis elegans.
1995,
Pubmed
Wang,
Hmx2 and Hmx3 homeobox genes direct development of the murine inner ear and hypothalamus and can be functionally replaced by Drosophila Hmx.
2004,
Pubmed
Wang,
Hmx: an evolutionary conserved homeobox gene family expressed in the developing nervous system in mice and Drosophila.
2000,
Pubmed
,
Echinobase
Wang,
Hmx homeobox gene function in inner ear and nervous system cell-type specification and development.
2005,
Pubmed
Wang,
Multiple regulatory changes contribute to the evolution of the Caenorhabditis lin-48 ovo gene.
2002,
Pubmed
Ward,
Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans.?2UU.
1975,
Pubmed
Wu,
Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation.
1994,
Pubmed
Yochem,
Ras is required for a limited number of cell fates and not for general proliferation in Caenorhabditis elegans.
1997,
Pubmed
Yoshimura,
mls-2 and vab-3 Control glia development, hlh-17/Olig expression and glia-dependent neurite extension in C. elegans.
2008,
Pubmed
Yoshiura,
Cloning, characterization, and mapping of the mouse homeobox gene Hmx1.
1998,
Pubmed
,
Echinobase