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The ubiquitin proteasome system (UPS) is the main proteolytic system of cells. Recent evidence suggests that the UPS plays a regulatory role in regeneration processes. Here, we explore the possibility that the UPS is involved during intestinal regeneration of the sea cucumber Holothuria glaberrima. These organisms can regenerate most of their digestive tract following a process of evisceration. Initially, we identified components of H. glaberrima UPS, including sequences for Rpn10, β3, and ubiquitin-RPL40. Predicted proteins from the mRNA sequences showed high degree of conservation that ranged from 60% (Rpn10) to 98% (Ub-RPL40). Microarrays and RT-PCR experiments showed that these genes were upregulated during intestinal regeneration. In addition, we demonstrated expression of alpha 20S proteasome subunits and ubiquitinated proteins during intestinal regeneration and detected them in the epithelium and connective tissue of the regenerating intestine. Finally, the intestinal regeneration was altered in animals treated with MG132, a proteasome inhibitor. These findings support our contention that proteasomes are playing an important role during intestinal regeneration.
Adori,
Subcellular distribution of components of the ubiquitin-proteasome system in non-diseased human and rat brain.
2006, Pubmed
Adori,
Subcellular distribution of components of the ubiquitin-proteasome system in non-diseased human and rat brain.
2006,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Awasthi,
Suppression of human lens epithelial cell proliferation by proteasome inhibition, a potential defense against posterior capsular opacification.
2006,
Pubmed
Baker,
The human ubiquitin-52 amino acid fusion protein gene shares several structural features with mammalian ribosomal protein genes.
1991,
Pubmed
Baumeister,
The proteasome: paradigm of a self-compartmentalizing protease.
1998,
Pubmed
Beal,
Surface hydrophobic residues of multiubiquitin chains essential for proteolytic targeting.
1996,
Pubmed
Bowerman,
Degrade to create: developmental requirements for ubiquitin-mediated proteolysis during early C. elegans embryogenesis.
2006,
Pubmed
Brooks,
Subcellular localization of proteasomes and their regulatory complexes in mammalian cells.
2000,
Pubmed
Cabrera-Serrano,
RGD-containing peptides inhibit intestinal regeneration in the sea cucumber Holothuria glaberrima.
2004,
Pubmed
,
Echinobase
Candelaria,
Contribution of mesenterial muscle dedifferentiation to intestine regeneration in the sea cucumber Holothuria glaberrima.
2006,
Pubmed
,
Echinobase
Chiba,
Activation of the proteasomes of sand dollar eggs at fertilization depends on the intracellular pH rise.
1999,
Pubmed
,
Echinobase
Clarke,
Proteolysis and the cell cycle.
2002,
Pubmed
Coux,
Structure and functions of the 20S and 26S proteasomes.
1996,
Pubmed
Dawson,
Developmental changes of the 26 S proteasome in abdominal intersegmental muscles of Manduca sexta during programmed cell death.
1995,
Pubmed
Elenich,
The complete primary structure of mouse 20S proteasomes.
1999,
Pubmed
El-Khodor,
The expression of mRNAs for the proteasome complex is developmentally regulated in the rat mesencephalon.
2001,
Pubmed
Feng,
Progressive sequence alignment as a prerequisite to correct phylogenetic trees.
1987,
Pubmed
Finley,
The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
1987,
Pubmed
Finley,
Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.
1998,
Pubmed
García-Arrarás,
Visceral regeneration in holothurians.
2001,
Pubmed
,
Echinobase
García-Arrarás,
Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrima Selenka (Holothuroidea:Echinodermata).
1998,
Pubmed
,
Echinobase
García-Mata,
Characterization and dynamics of aggresome formation by a cytosolic GFP-chimera.
1999,
Pubmed
Garrett,
Selective inhibitors of the osteoblast proteasome stimulate bone formation in vivo and in vitro.
2003,
Pubmed
Girão,
Subcellular redistribution of components of the ubiquitin-proteasome pathway during lens differentiation and maturation.
2005,
Pubmed
Glickman,
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.
2002,
Pubmed
Hamazaki,
Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development.
2007,
Pubmed
Hamilton,
Structure of a conjugating enzyme-ubiquitin thiolester intermediate reveals a novel role for the ubiquitin tail.
2001,
Pubmed
Hershko,
Roles of ubiquitin-mediated proteolysis in cell cycle control.
1997,
Pubmed
Hofmann,
A ubiquitin-interacting motif conserved in components of the proteasomal and lysosomal protein degradation systems.
2001,
Pubmed
Huson,
Dendroscope: An interactive viewer for large phylogenetic trees.
2007,
Pubmed
Iijima,
Participation of proteasomes in Xenopus embryogenesis.
2003,
Pubmed
Johnston,
Aggresomes: a cellular response to misfolded proteins.
1998,
Pubmed
Kapadia,
Nitric oxide regulates the 26S proteasome in vascular smooth muscle cells.
2009,
Pubmed
Kikukawa,
The 26S proteasome Rpn10 gene encoding splicing isoforms: evolutional conservation of the genomic organization in vertebrates.
2002,
Pubmed
Klein,
Cell-specific accumulation of Drosophila proteasomes (MCP) during early development.
1990,
Pubmed
Koistinen,
Extracellular matrix-induced changes in expression of cell cycle-related proteins and proteasome components in endometrial adenocarcinoma cells.
2006,
Pubmed
Lier,
The proteasome regulatory particle subunit Rpn6 is required for Drosophila development and interacts physically with signalosome subunit Alien/CSN2.
2002,
Pubmed
Lu,
Ubiquitylation and proteasomal degradation of the p21(Cip1), p27(Kip1) and p57(Kip2) CDK inhibitors.
2010,
Pubmed
Malloch,
Gene expression profiles of lens regeneration and development in Xenopus laevis.
2009,
Pubmed
Martín,
Immunohistochemical localization of protein gene product 9.5, ubiquitin, and neuropeptide Y immunoreactivities in epithelial and neuroendocrine cells from normal and hyperplastic human prostate.
2000,
Pubmed
Morimoto,
Cooperative Mesp activity is required for normal somitogenesis along the anterior-posterior axis.
2006,
Pubmed
Mtango,
Ubiquitin proteasome pathway gene expression varies in rhesus monkey oocytes and embryos of different developmental potential.
2007,
Pubmed
Mukherjee,
Pharmacologic targeting of a stem/progenitor population in vivo is associated with enhanced bone regeneration in mice.
2008,
Pubmed
Murray,
Myogenesis during holothurian intestinal regeneration.
2004,
Pubmed
,
Echinobase
Myung,
The ubiquitin-proteasome pathway and proteasome inhibitors.
2001,
Pubmed
Notredame,
T-Coffee: A novel method for fast and accurate multiple sequence alignment.
2000,
Pubmed
Ortiz-Pineda,
Gene expression profiling of intestinal regeneration in the sea cucumber.
2009,
Pubmed
,
Echinobase
Patruno,
Changes in ubiquitin conjugates and Hsp72 levels during arm regeneration in echinoderms.
2001,
Pubmed
Quiñones,
Extracellular matrix remodeling and metalloproteinase involvement during intestine regeneration in the sea cucumber Holothuria glaberrima.
2002,
Pubmed
,
Echinobase
Rao,
Proteomic analysis of blastema formation in regenerating axolotl limbs.
2009,
Pubmed
Rockel,
Proteasomes degrade proteins in focal subdomains of the human cell nucleus.
2005,
Pubmed
Rojas-Cartagena,
Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima.
2007,
Pubmed
,
Echinobase
Sawada,
Participation of proteasome-associating complex PC500 in starfish oocyte maturation as revealed by monoclonal antibodies.
2006,
Pubmed
,
Echinobase
Thompson,
Regulation of intestinal regeneration: new insights.
2000,
Pubmed
Tisdale,
The ubiquitin-proteasome pathway as a therapeutic target for muscle wasting.
2005,
Pubmed
Wallace,
M-Coffee: combining multiple sequence alignment methods with T-Coffee.
2006,
Pubmed
Wang,
Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity.
2010,
Pubmed
Wang,
Proteasome subunit LMP2 is required for matrix metalloproteinase-2 and -9 expression and activities in human invasive extravillous trophoblast cell line.
2006,
Pubmed
Wang,
Effect of ubiquitin-proteasome pathway on mouse blastocyst implantation and expression of matrix metalloproteinases-2 and -9.
2004,
Pubmed
Weng,
Gene expression profiling identifies regulatory pathways involved in the late stage of rat fetal lung development.
2006,
Pubmed
Wilkinson,
The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR studies on the complex between ubiquitin and UCH-L3.
1999,
Pubmed
Wójcik,
Intracellular localization of proteasomes.
2003,
Pubmed
Yin,
Proteasome inhibitor PS-341 causes cell growth arrest and apoptosis in human glioblastoma multiforme (GBM).
2005,
Pubmed
Yokota,
Sperm proteasomes are responsible for the acrosome reaction and sperm penetration of the vitelline envelope during fertilization of the sea urchin Pseudocentrotus depressus.
2007,
Pubmed
,
Echinobase
Young,
Characterization of two polyubiquitin binding sites in the 26 S protease subunit 5a.
1998,
Pubmed
