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PLoS One
2011 Jan 01;67:e22141. doi: 10.1371/journal.pone.0022141.
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Cellular renewal and improvement of local cell effector activity in peritoneal cavity in response to infectious stimuli.
Cassado Ados A
,
de Albuquerque JA
,
Sardinha LR
,
Buzzo Cde L
,
Faustino L
,
Nascimento R
,
Ghosn EE
,
Lima MR
,
Alvarez JM
,
Bortoluci KR
.
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The peritoneal cavity (PerC) is a singular compartment where many cell populations reside and interact. Despite the widely adopted experimental approach of intraperitoneal (i.p.) inoculation, little is known about the behavior of the different cell populations within the PerC. To evaluate the dynamics of peritoneal macrophage (MØ) subsets, namely small peritoneal MØ (SPM) and large peritoneal MØ (LPM), in response to infectious stimuli, C57BL/6 mice were injected i.p. with zymosan or Trypanosoma cruzi. These conditions resulted in the marked modification of the PerC myelo-monocytic compartment characterized by the disappearance of LPM and the accumulation of SPM and monocytes. In parallel, adherent cells isolated from stimulated PerC displayed reduced staining for β-galactosidase, a biomarker for senescence. Further, the adherent cells showed increased nitric oxide (NO) and higher frequency of IL-12-producing cells in response to subsequent LPS and IFN-γ stimulation. Among myelo-monocytic cells, SPM rather than LPM or monocytes, appear to be the central effectors of the activated PerC; they display higher phagocytic activity and are the main source of IL-12. Thus, our data provide a first demonstration of the consequences of the dynamics between peritoneal MØ subpopulations by showing that substitution of LPM by a robust SPM and monocytes in response to infectious stimuli greatly improves PerC effector activity.
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21799778
???displayArticle.pmcLink???PMC3142143 ???displayArticle.link???PLoS One
Aderem,
Mechanisms of phagocytosis in macrophages.
1999, Pubmed
Aderem,
Mechanisms of phagocytosis in macrophages.
1999,
Pubmed
Aliberti,
Host persistence: exploitation of anti-inflammatory pathways by Toxoplasma gondii.
2005,
Pubmed
Bastos,
Macrophages from IL-12p40-deficient mice have a bias toward the M2 activation profile.
2002,
Pubmed
Bastos,
What kind of message does IL-12/IL-23 bring to macrophages and dendritic cells?
2004,
Pubmed
Bastos,
Role of endogenous IFN-gamma in macrophage programming induced by IL-12 and IL-18.
2007,
Pubmed
Bogdan,
Production of interferon-gamma by myeloid cells--fact or fancy?
2006,
Pubmed
Cecílio,
Aging alters the production of iNOS, arginase and cytokines in murine macrophages.
2011,
Pubmed
Dimri,
A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
1995,
Pubmed
Dioszeghy,
12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
2008,
Pubmed
Edwards,
Biochemical and functional characterization of three activated macrophage populations.
2006,
Pubmed
Frucht,
IFN-gamma production by antigen-presenting cells: mechanisms emerge.
2001,
Pubmed
Geissmann,
Unravelling mononuclear phagocyte heterogeneity.
2010,
Pubmed
Geissmann,
Blood monocytes consist of two principal subsets with distinct migratory properties.
2003,
Pubmed
Ghosn,
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets.
2010,
Pubmed
Gordon,
Pattern recognition receptors: doubling up for the innate immune response.
2002,
Pubmed
Gordon,
Monocyte and macrophage heterogeneity.
2005,
Pubmed
Herrero,
Immunosenescence of macrophages: reduced MHC class II gene expression.
2002,
Pubmed
Herzenberg,
Interpreting flow cytometry data: a guide for the perplexed.
2006,
Pubmed
Hume,
Macrophages as APC and the dendritic cell myth.
2008,
Pubmed
Jung,
Detection of intracellular cytokines by flow cytometry.
1993,
Pubmed
Kovacs,
Aging and innate immunity in the mouse: impact of intrinsic and extrinsic factors.
2009,
Pubmed
Lloberas,
Effect of aging on macrophage function.
2002,
Pubmed
Mantovani,
The chemokine system in diverse forms of macrophage activation and polarization.
2004,
Pubmed
Martinez-Pomares,
Antigen presentation the macrophage way.
2007,
Pubmed
Mosser,
Exploring the full spectrum of macrophage activation.
2008,
Pubmed
Noël,
Alternatively activated macrophages during parasite infections.
2004,
Pubmed
Plackett,
Aging and innate immune cells.
2004,
Pubmed
Plowden,
Innate immunity in aging: impact on macrophage function.
2004,
Pubmed
Rodríguez-Sosa,
Chronic helminth infection induces alternatively activated macrophages expressing high levels of CCR5 with low interleukin-12 production and Th2-biasing ability.
2002,
Pubmed
Roederer,
Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.
2001,
Pubmed
Rosas,
The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B.
2010,
Pubmed
Schleicher,
Generation, culture and flow-cytometric characterization of primary mouse macrophages.
2009,
Pubmed
Schleicher,
Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-gamma in IL-12/IL-18-stimulated mouse macrophage populations.
2005,
Pubmed
Taylor,
Macrophage receptors and immune recognition.
2005,
Pubmed
Taylor,
Pattern recognition receptors and differentiation antigens define murine myeloid cell heterogeneity ex vivo.
2003,
Pubmed
Villacres-Eriksson,
Antigen presentation by naive macrophages, dendritic cells and B cells to primed T lymphocytes and their cytokine production following exposure to immunostimulating complexes.
1995,
Pubmed