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Dev Growth Differ
2011 Apr 01;533:427-39. doi: 10.1111/j.1440-169X.2011.01261.x.
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Concordance and interaction of guanine nucleotide dissociation inhibitor (RhoGDI) with RhoA in oogenesis and early development of the sea urchin.
Zazueta-Novoa V
,
Martínez-Cadena G
,
Wessel GM
,
Zazueta-Sandoval R
,
Castellano L
,
García-Soto J
.
???displayArticle.abstract??? Rho GTPases are Ras-related GTPases that regulate a variety of cellular processes. In the sea urchin Strongylocentrotus purpuratus, RhoA in the oocyte associates with the membrane of the cortical granules and directs their movement from the cytoplasm to the cell cortex during maturation to an egg. RhoA also plays an important role regulating the Na(+) -H(+) exchanger activity, which determines the internal pH of the cell during the first minutes of embryogenesis. We investigated how this activity may be regulated by a guanine-nucleotide dissociation inhibitor (RhoGDI). The sequence of this RhoA regulatory protein was identified in the genome on the basis of its similarity to other RhoGDI species, especially for key segments in the formation of the isoprenyl-binding pocket and in interactions with the Rho GTPase. We examined the expression and the subcellular localization of RhoGDI during oogenesis and in different developmental stages. We found that RhoGDI mRNA levels were high in eggs and during cleavage divisions until blastula, when it disappeared, only to reappear in gastrula stage. RhoGDI localization overlaps the presence of RhoA during oogenesis and in embryonic development, reinforcing the regulatory premise of the interaction. By use of recombinant protein interactions in vitro, we also find that these two proteins selectively interact. These results support the hypothesis of a functional relationship in vivo and now enable mechanistic insight for the cellular and organelle rearrangements that occur during oogenesis and embryonic development.
Abbott,
Calcium and the control of mammalian cortical granule exocytosis.
2001, Pubmed,
Echinobase
Abbott,
Calcium and the control of mammalian cortical granule exocytosis.
2001,
Pubmed
,
Echinobase
Abo,
Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1.
1991,
Pubmed
Adra,
RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas.
1997,
Pubmed
Allenspach,
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse.
2001,
Pubmed
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968,
Pubmed
,
Echinobase
Ando,
Post-translational processing of rac p21s is important both for their interaction with the GDP/GTP exchange proteins and for their activation of NADPH oxidase.
1992,
Pubmed
Arenas-Mena,
Spatial expression of Hox cluster genes in the ontogeny of a sea urchin.
2000,
Pubmed
,
Echinobase
Barrett,
The Rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation.
1997,
Pubmed
Beane,
RhoA regulates initiation of invagination, but not convergent extension, during sea urchin gastrulation.
2006,
Pubmed
,
Echinobase
Berg,
Cortical granules of the sea urchin translocate early in oocyte maturation.
1997,
Pubmed
,
Echinobase
Bishop,
Rho GTPases and their effector proteins.
2000,
Pubmed
Boguski,
Proteins regulating Ras and its relatives.
1993,
Pubmed
Caron,
Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases.
1998,
Pubmed
Chuang,
Biologically active lipids are regulators of Rac.GDI complexation.
1993,
Pubmed
Coso,
The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway.
1995,
Pubmed
Covián-Nares,
A Rho-signaling pathway mediates cortical granule translocation in the sea urchin oocyte.
2004,
Pubmed
,
Echinobase
Cuéllar-Mata,
The GTP-binding protein RhoA localizes to the cortical granules of Strongylocentrotus purpuratas sea urchin egg and is secreted during fertilization.
2000,
Pubmed
,
Echinobase
DerMardirossian,
GDIs: central regulatory molecules in Rho GTPase activation.
2005,
Pubmed
Fauré,
Interactions between Rho GTPases and Rho GDP dissociation inhibitor (Rho-GDI).
2001,
Pubmed
Fiedler,
Identification and characterization of RHOA-interacting proteins in bovine spermatozoa.
2008,
Pubmed
Fukumoto,
Molecular cloning and characterization of a novel type of regulatory protein (GDI) for the rho proteins, ras p21-like small GTP-binding proteins.
1990,
Pubmed
Golovanov,
Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI.
2001,
Pubmed
Gosser,
C-terminal binding domain of Rho GDP-dissociation inhibitor directs N-terminal inhibitory peptide to GTPases.
1997,
Pubmed
Grizot,
Crystal structure of the Rac1-RhoGDI complex involved in nadph oxidase activation.
2001,
Pubmed
Hill,
The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF.
1995,
Pubmed
Hiraoka,
Both stimulatory and inhibitory GDP/GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins.
1992,
Pubmed
Hoffman,
Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI.
2000,
Pubmed
Ihara,
Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue.
1998,
Pubmed
Isomura,
Regulation of binding of rhoB p20 to membranes by its specific regulatory protein, GDP dissociation inhibitor.
1991,
Pubmed
Jou,
Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.
2000,
Pubmed
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Keep,
A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm.
1997,
Pubmed
Kim,
Selective activation of small GTPase RhoA by tyrosine kinase Etk through its pleckstrin homology domain.
2002,
Pubmed
Knaus,
Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 2.
1991,
Pubmed
Kroschewski,
Cdc42 controls secretory and endocytic transport to the basolateral plasma membrane of MDCK cells.
1999,
Pubmed
LaFleur,
Sea urchin ovoperoxidase: oocyte-specific member of a heme-dependent peroxidase superfamily that functions in the block to polyspermy.
1998,
Pubmed
,
Echinobase
Laidlaw,
Cortical granule biogenesis is active throughout oogenesis in sea urchins.
1994,
Pubmed
,
Echinobase
Lamarche,
GAPs for rho-related GTPases.
1994,
Pubmed
Lamaze,
Regulation of receptor-mediated endocytosis by Rho and Rac.
1996,
Pubmed
Lelias,
cDNA cloning of a human mRNA preferentially expressed in hematopoietic cells and with homology to a GDP-dissociation inhibitor for the rho GTP-binding proteins.
1993,
Pubmed
Leonard,
The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein.
1992,
Pubmed
Leung,
Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA.
1999,
Pubmed
Longenecker,
How RhoGDI binds Rho.
1999,
Pubmed
Mabuchi,
Cleavage furrow: timing of emergence of contractile ring actin filaments and establishment of the contractile ring by filament bundling in sea urchin eggs.
1994,
Pubmed
,
Echinobase
Mackay,
Rho GTPases.
1998,
Pubmed
Manzo,
A Rho GTPase controls the rate of protein synthesis in the sea urchin egg.
2003,
Pubmed
,
Echinobase
Michaelson,
Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding.
2001,
Pubmed
Minden,
Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs.
1995,
Pubmed
Nishimura,
An IQGAP-like protein is involved in actin assembly together with Cdc42 in the sea urchin egg.
2003,
Pubmed
,
Echinobase
Nishimura,
Localization of Rho GTPase in sea urchin eggs.
1998,
Pubmed
,
Echinobase
Nobes,
Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.
1995,
Pubmed
Olofsson,
Rho guanine dissociation inhibitors: pivotal molecules in cellular signalling.
1999,
Pubmed
Qiu,
An essential role for Rac in Ras transformation.
1995,
Pubmed
Rangel-Mata,
Rho, Rho-kinase, and the actin cytoskeleton regulate the Na+ -H+ exchanger in sea urchin eggs.
2007,
Pubmed
,
Echinobase
Ransick,
Whole mount in situ hybridization shows Endo 16 to be a marker for the vegetal plate territory in sea urchin embryos.
1993,
Pubmed
,
Echinobase
Regazzi,
Characterization of small-molecular-mass guanine-nucleotide-binding regulatory proteins in insulin-secreting cells and PC12 cells.
1992,
Pubmed
Ridley,
The small GTP-binding protein rac regulates growth factor-induced membrane ruffling.
1992,
Pubmed
Ridley,
Rho family proteins: coordinating cell responses.
2001,
Pubmed
Ridley,
The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.
1992,
Pubmed
Rivero,
The Dictyostelium discoideum family of Rho-related proteins.
2001,
Pubmed
Sathananthan,
The origin and distribution of cortical granules in human oocytes with reference to Golgi, nucleolar, and microfilament activity.
1985,
Pubmed
Schatten,
Microtubules are required for centrosome expansion and positioning while microfilaments are required for centrosome separation in sea urchin eggs during fertilization and mitosis.
1988,
Pubmed
,
Echinobase
Scheffzek,
The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI.
2000,
Pubmed
Scherle,
Ly-GDI, a GDP-dissociation inhibitor of the RhoA GTP-binding protein, is expressed preferentially in lymphocytes.
1993,
Pubmed
Sun,
Regulation of dynamic events by microfilaments during oocyte maturation and fertilization.
2006,
Pubmed
Symons,
Control of vesicular trafficking by Rho GTPases.
2003,
Pubmed
Takahashi,
Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein.
1997,
Pubmed
Ueda,
Purification and characterization from bovine brain cytosol of a novel regulatory protein inhibiting the dissociation of GDP from and the subsequent binding of GTP to rhoB p20, a ras p21-like GTP-binding protein.
1990,
Pubmed
Wei,
Crystal structure of RhoA-GDP and its functional implications.
1997,
Pubmed
Wessel,
Cortical granule translocation is microfilament mediated and linked to meiotic maturation in the sea urchin oocyte.
2002,
Pubmed
,
Echinobase
Wessel,
Cell surface changes in the egg at fertilization.
2009,
Pubmed
,
Echinobase
Wessel,
The biology of cortical granules.
2001,
Pubmed
Wessel,
SFE1, a constituent of the fertilization envelope in the sea urchin is made by oocytes and contains low-density lipoprotein-receptor-like repeats.
2000,
Pubmed
,
Echinobase
Yamashita,
The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI.
2003,
Pubmed
Zalcman,
RhoGDI-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG.
1996,
Pubmed
Zalcman,
RhoGAPs and RhoGDIs, (His)stories of two families.
1999,
Pubmed
Zong,
Loop 6 of RhoA confers specificity for effector binding, stress fiber formation, and cellular transformation.
1999,
Pubmed
