Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Bioeng Bugs
2010 Jan 01;13:217-20. doi: 10.4161/bbug.1.3.11721.
Show Gene links
Show Anatomy links
Powerful workhorses for antimicrobial peptide expression and characterization.
Li C
,
Blencke HM
,
Paulsen V
,
Haug T
,
Stensvåg K
.
???displayArticle.abstract???
Discovery of antimicrobial peptides (AMP) is to a large extent based on screening of fractions of natural samples in bacterial growth inhibition assays. However, the use of bacteria is not limited to screening for antimicrobial substances. In later steps, bioengineered "bugs" can be applied to both production and characterization of AMPs. Here we describe the idea to use genetically modified Escherichia coli strains for both these purposes. This approach allowed us to investigate SpStrongylocins 1 and 2 from the purple sea urchin Strongylocentrotus purpuratus only based on sequence information from a cDNA library and without previous direct isolation or chemical synthesis of these peptides. The recombinant peptides are proved active against all bacterial strains tested. An assay based on a recombinant E. coli sensor strain expressing insect luciferase, revealed that SpStrongylocins are not interfering with membrane integrity and are therefore likely to have intracellular targets.
Ankö,
An Escherichia coli biosensor strain for amplified and high throughput detection of antimicrobial agents.
2002, Pubmed
Ankö,
An Escherichia coli biosensor strain for amplified and high throughput detection of antimicrobial agents.
2002,
Pubmed
Aricescu,
A time- and cost-efficient system for high-level protein production in mammalian cells.
2006,
Pubmed
Baneyx,
Recombinant protein expression in Escherichia coli.
1999,
Pubmed
Berthold,
Screening for functional expression and overexpression of a family of diiron-containing interfacial membrane proteins using the univector recombination system.
2003,
Pubmed
Boettner,
High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.
2002,
Pubmed
Bordes,
A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica.
2007,
Pubmed
Bowdish,
A re-evaluation of the role of host defence peptides in mammalian immunity.
2005,
Pubmed
Brogden,
Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
2005,
Pubmed
Carballar-Lejarazú,
Recombinant scorpine: a multifunctional antimicrobial peptide with activity against different pathogens.
2008,
Pubmed
Caron,
Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins.
2009,
Pubmed
Chiou,
Stable expression in a Chinese hamster ovary (CHO) cell line of bioactive recombinant chelonianin, which plays an important role in protecting fish against pathogenic infection.
2009,
Pubmed
Cui,
Production of recombinant orange-spotted grouper (Epinephelus coioides) luteinizing hormone in insect cells by the baculovirus expression system and its biological effect.
2007,
Pubmed
Destoumieux,
Recombinant expression and range of activity of penaeidins, antimicrobial peptides from penaeid shrimp.
1999,
Pubmed
Dumon-Seignovert,
The toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3).
2004,
Pubmed
Esposito,
Enhancement of soluble protein expression through the use of fusion tags.
2006,
Pubmed
Galluzzi,
Amplified detection of transcriptional and translational inhibitors in bioluminescent Escherichia coli K-12.
2003,
Pubmed
Hancock,
Host defence peptides from invertebrates--emerging antimicrobial strategies.
2006,
Pubmed
Haught,
Recombinant production and purification of novel antisense antimicrobial peptide in Escherichia coli.
1998,
Pubmed
Holz,
A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae.
2002,
Pubmed
Huang,
Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression.
2008,
Pubmed
Jordan,
Baculovirus-infected insect cells expressing peptide-MHC complexes elicit protective antitumor immunity.
2008,
Pubmed
Kim,
Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli.
2006,
Pubmed
Kremer,
Biochemical characterization of acyl carrier protein (AcpM) and malonyl-CoA:AcpM transacylase (mtFabD), two major components of Mycobacterium tuberculosis fatty acid synthase II.
2001,
Pubmed
Kumar,
Cloning, direct expression, and purification of a snake venom cardiotoxin in Escherichia coli.
1996,
Pubmed
Lampinen,
Use of controlled luciferase expression to monitor chemicals affecting protein synthesis.
1995,
Pubmed
Lee,
Acidic peptide-mediated expression of the antimicrobial peptide buforin II as tandem repeats in Escherichia coli.
1998,
Pubmed
Lee,
High-level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies.
2000,
Pubmed
Li,
Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria.
2010,
Pubmed
,
Echinobase
Li,
Strongylocins, novel antimicrobial peptides from the green sea urchin, Strongylocentrotus droebachiensis.
2008,
Pubmed
,
Echinobase
Mancia,
Optimization of protein production in mammalian cells with a coexpressed fluorescent marker.
2004,
Pubmed
Miroux,
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.
1996,
Pubmed
Mookherjee,
Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections.
2007,
Pubmed
Moon,
Expression and purification of a recombinant LL-37 from Escherichia coli.
2006,
Pubmed
Morassutti,
Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
2002,
Pubmed
Morlot,
Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3.
2007,
Pubmed
Piers,
Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria.
1993,
Pubmed
Pietiäinen,
Transcriptome analysis of the responses of Staphylococcus aureus to antimicrobial peptides and characterization of the roles of vraDE and vraSR in antimicrobial resistance.
2009,
Pubmed
Pruzinská,
Chlorophyll breakdown: pheophorbide a oxygenase is a Rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene.
2003,
Pubmed
Rajalingam,
Prohibitin is required for Ras-induced Raf-MEK-ERK activation and epithelial cell migration.
2005,
Pubmed
Skosyrev,
Expression of the recombinant antibacterial peptide sarcotoxin IA in Escherichia coli cells.
2003,
Pubmed
Skosyrev,
EGFP as a fusion partner for the expression and organic extraction of small polypeptides.
2003,
Pubmed
Smith,
Production of human beta interferon in insect cells infected with a baculovirus expression vector.
1983,
Pubmed
Tomasinsig,
Genome-wide transcriptional profiling of the Escherichia coli response to a proline-rich antimicrobial peptide.
2004,
Pubmed
Virta,
Real-time measurement of cell permeabilization with low-molecular-weight membranolytic agents.
1995,
Pubmed
Vollmer,
Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.
1997,
Pubmed
Wang,
APD2: the updated antimicrobial peptide database and its application in peptide design.
2009,
Pubmed
Wong,
Hot-spot mutants of p53 core domain evince characteristic local structural changes.
1999,
Pubmed
Yang,
Expression and localization of recombinant human EDG-1 receptors in Pichia pastoris.
2006,
Pubmed
Zhang,
Determinants of recombinant production of antimicrobial cationic peptides and creation of peptide variants in bacteria.
1998,
Pubmed
Zhang,
Expression and preparation of recombinant hepcidin in Escherichia coli.
2005,
Pubmed
