Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-41661
Acta Derm Venereol 2010 May 01;903:235-8. doi: 10.2340/00015555-0783.
Show Gene links Show Anatomy links

Identification of a major yolk protein as an allergen in sea urchin roe.

Yamasaki A , Higaki H , Nakashima K , Yamamoto O , Hein KZ , Takahashi H , Chinuki Y , Morita E .


???displayArticle.abstract???
Anaphylaxis after eating sea urchin roe has been reported. However, its major allergens have not yet been identified. The aim of this study was to identify the major allergens of sea urchin roe. Proteins of sea urchin roe were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis (2-DE). An immunoglobulin (Ig)E-binding protein was detected by immunoblotting using the patient''s serum. An allergen isolated from 2DE-gel was identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Immunoblot analysis of sea urchin extracts showed that a 160-kDa protein at pI 6-7 was recognized by the patient''s IgE. Peptide mass fingerprint analysis revealed that the protein was the major yolk protein (152 kDa, pI 6.9) of sea urchins. The results show that a major allergen of sea urchin roe is the major yolk protein.

???displayArticle.pubmedLink??? 20526538
???displayArticle.link??? Acta Derm Venereol


Genes referenced: LOC100887844