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Cell Mol Life Sci
2010 Jun 01;6711:1871-9. doi: 10.1007/s00018-010-0297-7.
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Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus.
Bellavia D
,
Barbieri R
.
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In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling.
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