ECB-ART-41415
Annu Int Conf IEEE Eng Med Biol Soc
2009 Jan 01;2009:970-3. doi: 10.1109/IEMBS.2009.5333756.
Show Gene links
Show Anatomy links
Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements.
???displayArticle.abstract???
We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.
???displayArticle.pubmedLink??? 19964254
???displayArticle.link??? Annu Int Conf IEEE Eng Med Biol Soc
Genes referenced: LOC100887844 LOC115919910