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Environ Sci Technol
2009 Nov 01;4321:8374-80. doi: 10.1021/es901677r.
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Multidrug efflux transporters limit accumulation of inorganic, but not organic, mercury in sea urchin embryos.
Bosnjak I, Uhlinger KR, Heim W, Smital T, Franekić-Colić J, Coale K, Epel D, Hamdoun A.
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Mercuric compounds are persistent global pollutants that accumulate in marine organisms and in humans who consume them. While the chemical cycles and speciation of mercury in the oceans are relatively well described, the cellular mechanisms that govern which forms of mercury accumulate in cells and why they persist are less understood. In this study we examined the role of multidrug efflux transport in the differential accumulation of inorganic (HgCl(2)) and organic (CH(3)HgCl) mercury in sea urchin (Strongylocentrotus purpuratus) embryos. We found that inhibition of MRP/ABCC-type transporters increases intracellular accumulation of inorganic mercury but had no effect on accumulation of organic mercury. Similarly, pharmacological inhibition of metal conjugating enzymes by ligands GST/GSH significantly increases this antimitotic potency of inorganic mercury, but had no effect on the potency of organic mercury. Our results point to MRP-mediated elimination of inorganic mercury conjugates as a cellular basis for differences in the accumulation and potency of the two major forms of mercury found in marine environments.
Abbott,
Transporting therapeutics across the blood-brain barrier.
1996, Pubmed
Abbott,
Transporting therapeutics across the blood-brain barrier.
1996,
Pubmed Aleo,
Endogenous thiols and MRP transporters contribute to Hg2+ efflux in HgCl2-treated tubular MDCK cells.
2005,
Pubmed Ambudkar,
Biochemical, cellular, and pharmacological aspects of the multidrug transporter.
1999,
Pubmed Aslamkhan,
Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells.
2003,
Pubmed Ballatori,
Molecular mechanisms of reduced glutathione transport: role of the MRP/CFTR/ABCC and OATP/SLC21A families of membrane proteins.
2005,
Pubmed Ballatori,
Biliary secretion of glutathione and of glutathione-metal complexes.
1985,
Pubmed Bridges,
MRP2 and the DMPS- and DMSA-mediated elimination of mercury in TR(-) and control rats exposed to thiol S-conjugates of inorganic mercury.
2008,
Pubmed Bridges,
Molecular and ionic mimicry and the transport of toxic metals.
2005,
Pubmed Broeks,
Homologues of the human multidrug resistance genes MRP and MDR contribute to heavy metal resistance in the soil nematode Caenorhabditis elegans.
1996,
Pubmed Clarkson,
The toxicology of mercury--current exposures and clinical manifestations.
2003,
Pubmed Cole,
Transport of glutathione and glutathione conjugates by MRP1.
2006,
Pubmed Dean,
The human ATP-binding cassette (ABC) transporter superfamily.
2001,
Pubmed Epel,
Efflux transporters: newly appreciated roles in protection against pollutants.
2008,
Pubmed Essodaigui,
Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein.
1998,
Pubmed Foltz,
Echinoderm eggs and embryos: procurement and culture.
2004,
Pubmed
,
Echinobase Goldstone,
The chemical defensome: environmental sensing and response genes in the Strongylocentrotus purpuratus genome.
2006,
Pubmed
,
Echinobase Hamdoun,
Activation of multidrug efflux transporter activity at fertilization in sea urchin embryos (Strongylocentrotus purpuratus).
2004,
Pubmed
,
Echinobase Hayes,
Glutathione transferases.
2005,
Pubmed Holló,
Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells.
1996,
Pubmed Ishikawa,
Coordinated induction of MRP/GS-X pump and gamma-glutamylcysteine synthetase by heavy metals in human leukemia cells.
1996,
Pubmed Keppler,
Transport function and substrate specificity of multidrug resistance protein.
1998,
Pubmed Kerper,
Methylmercury transport across the blood-brain barrier by an amino acid carrier.
1992,
Pubmed Kim,
Mercury induces multidrug resistance-associated protein gene through p38 mitogen-activated protein kinase.
2005,
Pubmed Klein,
The multidrug resistance-associated protein (MRP/ABCC) subfamily of ATP-binding cassette transporters in plants.
2006,
Pubmed Leslie,
Toxicological relevance of the multidrug resistance protein 1, MRP1 (ABCC1) and related transporters.
2001,
Pubmed Li,
The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump.
1996,
Pubmed Luckenbach,
Nitromusk and polycyclic musk compounds as long-term inhibitors of cellular xenobiotic defense systems mediated by multidrug transporters.
2005,
Pubmed Mackay,
Correlation of bioconcentration factors.
1982,
Pubmed Miller,
Nucleoside phosphonate interactions with multiple organic anion transporters in renal proximal tubule.
2001,
Pubmed Miller,
MRP2 and acquired tolerance to inorganic arsenic in the kidney of killifish (Fundulus heteroclitus).
2007,
Pubmed Ochi,
Methylmercury, but not inorganic mercury, causes abnormality of centrosome integrity (multiple foci of gamma-tubulin), multipolar spindles and multinucleated cells without microtubule disruption in cultured Chinese hamster V79 cells.
2002,
Pubmed Pfeffer,
Properties of tubulin in unfertilized sea urchin eggs. Quantitation and characterization by the colchicine-binding reaction.
1976,
Pubmed
,
Echinobase Salinas,
Glutathione S-transferases--a review.
1999,
Pubmed Shapiro,
The control of oxidant stress at fertilization.
1991,
Pubmed
,
Echinobase Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase Turner,
Ovothiols, a family of redox-active mercaptohistidine compounds from marine invertebrate eggs.
1987,
Pubmed
,
Echinobase Vogel,
The effects of methyl mercury binding to microtubules.
1985,
Pubmed Yole,
Cell death and cytotoxic effects in YAC-1 lymphoma cells following exposure to various forms of mercury.
2007,
Pubmed Zalups,
MRP2 involvement in renal proximal tubular elimination of methylmercury mediated by DMPS or DMSA.
2009,
Pubmed Zaman,
Role of glutathione in the export of compounds from cells by the multidrug-resistance-associated protein.
1995,
Pubmed
