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Plant Physiol
2009 Oct 01;1512:820-9. doi: 10.1104/pp.109.142067.
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Extracellular DNA is required for root tip resistance to fungal infection.
Wen F
,
White GJ
,
VanEtten HD
,
Xiong Z
,
Hawes MC
.
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Plant defense involves a complex array of biochemical interactions, many of which occur in the extracellular environment. The apical 1- to 2-mm root tip housing apical and root cap meristems is resistant to infection by most pathogens, so growth and gravity sensing often proceed normally even when other sites on the root are invaded. The mechanism of this resistance is unknown but appears to involve a mucilaginous matrix or "slime" composed of proteins, polysaccharides, and detached living cells called "border cells." Here, we report that extracellular DNA (exDNA) is a component of root cap slime and that exDNA degradation during inoculation by a fungal pathogen results in loss of root tip resistance to infection. Most root tips (>95%) escape infection even when immersed in inoculum from the root-rotting pathogen Nectria haematococca. By contrast, 100% of inoculated root tips treated with DNase I developed necrosis. Treatment with BAL31, an exonuclease that digests DNA more slowly than DNase I, also resulted in increased root tip infection, but the onset of infection was delayed. Control root tips or fungal spores treated with nuclease alone exhibited normal morphology and growth. Pea (Pisum sativum) root tips incubated with [(32)P]dCTP during a 1-h period when no cell death occurs yielded root cap slime containing (32)P-labeled exDNA. Our results suggest that exDNA is a previously unrecognized component of plant defense, an observation that is in accordance with the recent discovery that exDNA from white blood cells plays a key role in the vertebrate immune response against microbial pathogens.
Allesen-Holm,
A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms.
2006, Pubmed
Allesen-Holm,
A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms.
2006,
Pubmed
Braun,
THE EFFECTS OF DNA AND ENZYME-TREATED DNA ON BACTERIAL POPULATION CHANGES.
1954,
Pubmed
Brigham,
Differential Expression of Proteins and mRNAs from Border Cells and Root Tips of Pea.
1995,
Pubmed
Brinkmann,
Beneficial suicide: why neutrophils die to make NETs.
2007,
Pubmed
Brinkmann,
Neutrophil extracellular traps kill bacteria.
2004,
Pubmed
Buchanan,
DNase expression allows the pathogen group A Streptococcus to escape killing in neutrophil extracellular traps.
2006,
Pubmed
CATLIN,
Extracellular deoxyribonucleic acid of bacteria and a deoxyribonuclease inhibitor.
1956,
Pubmed
Caetano-Anollés,
Growth and Movement of Spot Inoculated Rhizobium meliloti on the Root Surface of Alfalfa.
1992,
Pubmed
Carroll,
Ca2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts.
1998,
Pubmed
Clark,
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
1988,
Pubmed
Cooper,
Biosynthetic labeling with 32P: radiation damage to mammalian cells.
1985,
Pubmed
De-la-Peña,
Root-microbe communication through protein secretion.
2008,
Pubmed
Fahy,
Markers of mucus secretion and DNA levels in induced sputum from asthmatic and from healthy subjects.
1993,
Pubmed
Guimarães-Costa,
Leishmania amazonensis promastigotes induce and are killed by neutrophil extracellular traps.
2009,
Pubmed
Gunawardena,
Tissue-specific localization of pea root infection by Nectria haematococca. Mechanisms and consequences.
2005,
Pubmed
,
Echinobase
Gunawardena,
Tissue specific localization of root infection by fungal pathogens: role of root border cells.
2002,
Pubmed
,
Echinobase
Hawes,
Function of root border cells in plant health: pioneers in the rhizosphere.
1998,
Pubmed
Hawes,
The role of root border cells in plant defense.
2000,
Pubmed
Izano,
Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms.
2008,
Pubmed
James Morré,
Golgi apparatus mediated polysaccharide secretion by outer root cap cells of Zea mays : I. Kinetics and secretory pathway.
1967,
Pubmed
Janska,
Stoichiometric shifts in the common bean mitochondrial genome leading to male sterility and spontaneous reversion to fertility.
1998,
Pubmed
Jones,
Fluorescence microplate-based assay for tumor necrosis factor activity using SYTOX Green stain.
2001,
Pubmed
Jones,
Development of the quiescent center in maturing embryonic radicles of pea (Pisum sativum L. cv. Alaska).
1977,
Pubmed
Klosterman,
Characterization of a 20 kDa DNase elicitor from Fusarium solani f. sp. phaseoli and its expression at the onset of induced resistance in Pisum sativum.
2001,
Pubmed
Knee,
Root mucilage from pea and its utilization by rhizosphere bacteria as a sole carbon source.
2001,
Pubmed
Kraszewska,
Extrachromosomal DNA of pea-root (Pisum sativum) has repeated sequences and ribosomal genes.
1985,
Pubmed
Krimer,
Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement.
1983,
Pubmed
Kubista,
Characterization of interaction between DNA and 4',6-diamidino-2-phenylindole by optical spectroscopy.
1987,
Pubmed
Kwon,
Secretory pathways in plant immune responses.
2008,
Pubmed
Leuchtenberger,
Variation in the Amounts of Desoxyribose Nucleic Acid (DNA) in Cells of the Same Tissue and its Correlation with Secretory Function.
1952,
Pubmed
Lynch,
Domain-specific and cell type-specific localization of two types of cell wall matrix polysaccharides in the clover root tip.
1992,
Pubmed
Macas,
Repetitive DNA in the pea (Pisum sativum L.) genome: comprehensive characterization using 454 sequencing and comparison to soybean and Medicago truncatula.
2007,
Pubmed
Medina,
Neutrophil extracellular traps: a strategic tactic to defeat pathogens with potential consequences for the host.
2009,
Pubmed
Miao,
Identification and chromosomal locations of a family of cytochrome P-450 genes for pisatin detoxification in the fungus Nectria haematococca.
1991,
Pubmed
,
Echinobase
Miyasaka,
Possible role of root border cells in detection and avoidance of aluminum toxicity.
2001,
Pubmed
Palić,
Fish cast NETs: neutrophil extracellular traps are released from fish neutrophils.
2007,
Pubmed
Phillips,
Deoxyribonucleic Acid synthesis in root cap cells of cultured roots of convolvulus.
1971,
Pubmed
Qin,
Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis.
2007,
Pubmed
Rougier,
Use of lectin to detect the sugar components of maize root cap slime.
1979,
Pubmed
SHERRY,
The extent of the enzymatic degradation of desoxyribonucleic acid (DNA) in purulent exudates by streptodornase.
1950,
Pubmed
SMITHIES,
The deoxyribose nucleic acid slime layer of some halophilic bacteria.
1955,
Pubmed
Shittu,
Plant-endophyte interplay protects tomato against a virulent Verticillium.
2009,
Pubmed
Spoering,
Quorum sensing and DNA release in bacterial biofilms.
2006,
Pubmed
Sumby,
Extracellular deoxyribonuclease made by group A Streptococcus assists pathogenesis by enhancing evasion of the innate immune response.
2005,
Pubmed
Sweigard,
Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea.
1992,
Pubmed
Tavares,
DNase-resistant DNA in the extracellular and cell wall-associated fractions of Frankia strains R43 and CcI3.
2001,
Pubmed
Urban,
Neutrophil extracellular traps capture and kill Candida albicans yeast and hyphal forms.
2006,
Pubmed
Van't Hof,
Cells of pea (Pisum sativum) that differentiate from G2 phase have extrachromosomal DNA.
1982,
Pubmed
Wartha,
Neutrophil extracellular traps: casting the NET over pathogenesis.
2007,
Pubmed
Wen,
Extracellular proteins in pea root tip and border cell exudates.
2007,
Pubmed
,
Echinobase
Yousefi,
Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense.
2008,
Pubmed
von Köckritz-Blickwede,
Immunological mechanisms underlying the genetic predisposition to severe Staphylococcus aureus infection in the mouse model.
2008,
Pubmed
