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Biophys J
2009 Mar 04;965:1944-51. doi: 10.1016/j.bpj.2008.10.070.
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Chromatin stability at low concentration depends on histone octamer saturation levels.
Hagerman TA
,
Fu Q
,
Molinié B
,
Denvir J
,
Lindsay S
,
Georgel PT
.
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Studies on the stability of nucleosome core particles as a function of concentration have indicated a lower limit of approximately 5 ng/microL, below which the complexes start to spontaneously destabilize. Until recently little information was available on the effect of low concentration on chromatin. Using the well-characterized array of tandemly repeated 5S rDNA reconstituted into chromatin, we have investigated the effect of dilution. In this study, we demonstrate that the stability of saturated nucleosomal arrays and that of nucleosome core particles are within the same order of magnitude, and no significant loss of histones is monitored down to a concentration of 2.5 ng/microL. We observed that levels of subsaturation of the nucleosomal arrays were directly correlated with an increased sensitivity to histone loss, suggesting a shielding effect. The loss of histones from our linear nucleosomal arrays was shown not to be random, with a significant likelihood to occur at the end of the template than toward the center. This observation indicates that centrally located nucleosomes are more stable than those close to the end of the DNA templates. Itis important to take this information into account for the proper design of experiments pertaining to histone composition and the folding ability of chromatin samples.
Adkins,
Characterization of chromatin samples in the presence of Drosophila embryo extract by quantitative agarose gel electrophoresis.
2006, Pubmed
Adkins,
Characterization of chromatin samples in the presence of Drosophila embryo extract by quantitative agarose gel electrophoresis.
2006,
Pubmed
Adkins,
The use of Quantitative Agarose Gel Electrophoresis for rapid analysis of the integrity of protein-DNA complexes.
2007,
Pubmed
,
Echinobase
Ausio,
Nucleosome core particle stability and conformational change. Effect of temperature, particle and NaCl concentrations, and crosslinking of histone H3 sulfhydryl groups.
1984,
Pubmed
Ausio,
Histone hyperacetylation: its effects on nucleosome conformation and stability.
1986,
Pubmed
Chen,
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
1994,
Pubmed
Choudhary,
ATP-dependent chromatin remodelling: action and reaction.
2007,
Pubmed
Claudet,
Histone octamer instability under single molecule experiment conditions.
2005,
Pubmed
Cotton,
Nucleosome dissociation at physiological ionic strengths.
1981,
Pubmed
Cui,
Pulling a single chromatin fiber reveals the forces that maintain its higher-order structure.
2000,
Pubmed
Culp,
Regulation of mucous acinar exocrine secretion with age.
1996,
Pubmed
Eisenberg,
Hydrodynamic studies of the interaction between nucleosome core particles and core histones.
1981,
Pubmed
Fletcher,
Quantitative agarose gel electrophoresis of chromatin: nucleosome-dependent changes in charge, sharp, and deformability at low ionic strength.
1994,
Pubmed
Georgel,
Chromatin structure of eukaryotic promoters: a changing perspective.
2002,
Pubmed
Georgel,
Quantitative characterization of specific genomic promoters using agarose gel electrophoresis.
2003,
Pubmed
Georgel,
Binding of the RNA polymerase I transcription complex to its promoter can modify positioning of downstream nucleosomes assembled in vitro.
1993,
Pubmed
,
Echinobase
Godde,
Disruption of reconstituted nucleosomes. The effect of particle concentration, MgCl2 and KCl concentration, the histone tails, and temperature.
1995,
Pubmed
Gottesfeld,
Energetics and affinity of the histone octamer for defined DNA sequences.
2001,
Pubmed
,
Echinobase
Hansen,
Conformational dynamics of the chromatin fiber in solution: determinants, mechanisms, and functions.
2002,
Pubmed
Hansen,
The mechanism of nucleosome assembly onto oligomers of the sea urchin 5 S DNA positioning sequence.
1991,
Pubmed
,
Echinobase
Hansen,
Assembly and structural properties of subsaturated chromatin arrays.
1993,
Pubmed
Hansen,
Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.
1989,
Pubmed
,
Echinobase
Imbalzano,
Facilitated binding of TATA-binding protein to nucleosomal DNA.
1994,
Pubmed
Li,
Nucleosomes facilitate their own invasion.
2004,
Pubmed
Libertini,
Effects of pH on low-salt transition of chromatin core particles.
1982,
Pubmed
Lilley,
Structure and function of chromatin.
1979,
Pubmed
Lohr,
Using atomic force microscopy to study chromatin structure and nucleosome remodeling.
2007,
Pubmed
Marky,
A theory of DNA dissociation from the nucleosome.
1995,
Pubmed
Schneider,
Dynamics and interplay of nuclear architecture, genome organization, and gene expression.
2007,
Pubmed
Schwarz,
Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
1996,
Pubmed
Shen,
A chromatin remodelling complex involved in transcription and DNA processing.
2000,
Pubmed
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
,
Echinobase
Sugawara,
In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.
2003,
Pubmed
Thåström,
Measurement of histone-DNA interaction free energy in nucleosomes.
2004,
Pubmed
Tsukuda,
Chromatin remodelling at a DNA double-strand break site in Saccharomyces cerevisiae.
2005,
Pubmed
Usui,
A DNA damage response pathway controlled by Tel1 and the Mre11 complex.
2001,
Pubmed
Walker,
The thermal denaturation of chromatin core particles.
1984,
Pubmed
Woodcock,
Higher-order structure of chromatin and chromosomes.
2001,
Pubmed
Workman,
Nucleosome core displacement in vitro via a metastable transcription factor-nucleosome complex.
1992,
Pubmed
Yager,
Dynamics and equilibria of nucleosomes at elevated ionic strength.
1984,
Pubmed
Zama,
Nucleosome conformation: pH and organic solvent effects.
1978,
Pubmed