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Mar Biotechnol (NY)
2009 Jan 01;116:686-98. doi: 10.1007/s10126-009-9182-5.
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First insights into the biochemistry of tube foot adhesive from the sea urchin Paracentrotus lividus (Echinoidea, Echinodermata).
Santos R
,
da Costa G
,
Franco C
,
Gomes-Alves P
,
Flammang P
,
Coelho AV
.
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Sea urchins are common inhabitants of wave-swept shores. To withstand the action of waves, they rely on highly specialized independent adhesive organs, the adoral tube feet. The latter are extremely well-designed for temporary adhesion being composed by two functional subunits: (1) an apical disc that produces an adhesive secretion to fasten the sea urchin to the substratum, as well as a deadhesive secretion to allow the animal to move and (2) a stem that bears the tensions placed on the animal by hydrodynamism. Despite their technological potential for the development of new biomimetic underwater adhesives, very little is known about the biochemical composition of sea urchin adhesives. A characterization of sea urchin adhesives is presented using footprints. The latter contain inorganic residues (45.5%), proteins (6.4%), neutral sugars (1.2%), and lipids (2.5%). Moreover, the amino acid composition of the soluble protein fraction revealed a bias toward six amino acids: glycine, alanine, valine, serine, threonine, and asparagine/aspartic acid, which comprise 56.8% of the total residues. In addition, it also presents higher levels of proline (6.8%) and half-cystine (2.6%) than average eukaryotic proteins. Footprint insolubility was partially overcome using strong denaturing and reducing buffers, enabling the visualization of 13 proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The conjugation of mass spectrometry with homology-database search allowed the identification of six proteins: alpha and beta tubulin, actin, and histones H2B, H3, H2A, and H4, whose location and function in the adhesive are discussed but require further investigation. For the remaining unidentified proteins, five de novo-generated peptide sequences were found that were not present in the available protein databases, suggesting that they might be novel or modified proteins.
Cameron,
A sea urchin genome project: sequence scan, virtual map, and additional resources.
2000, Pubmed,
Echinobase
Cameron,
A sea urchin genome project: sequence scan, virtual map, and additional resources.
2000,
Pubmed
,
Echinobase
Candiano,
Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis.
2004,
Pubmed
DeMoor,
Characterization of the adhesive from cuvierian tubules of the sea cucumber Holothuria forskali (Echinodermata, Holothuroidea).
2003,
Pubmed
,
Echinobase
Flammang,
Echinoderm adhesive secretions: from experimental characterization to biotechnological applications.
2005,
Pubmed
,
Echinobase
Flammang,
A study of the temporary adhesion of the podia in the sea star asterias rubens (Echinodermata, asteroidea) through their footprints.
1998,
Pubmed
,
Echinobase
Galli,
Life on the wire: on tensegrity and force balance in cells.
2005,
Pubmed
Gobom,
Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry.
1999,
Pubmed
Hamwood,
Preliminary characterisation and extraction of anterior adhesive secretion in monogenean (platyhelminth) parasites.
2002,
Pubmed
,
Echinobase
Henriquez,
Extracellular matrix histone H1 binds to perlecan, is present in regenerating skeletal muscle and stimulates myoblast proliferation.
2002,
Pubmed
Kamino,
Barnacle cement proteins. Importance of disulfide bonds in their insolubility.
2000,
Pubmed
Kamino,
Underwater adhesive of marine organisms as the vital link between biological science and material science.
2008,
Pubmed
Kamino,
Cement proteins of the acorn barnacle, Megabalanus rosa.
1996,
Pubmed
Kohnke-Godt,
Heparin-binding lectin from human placenta: further characterization of ligand binding and structural properties and its relationship to histones and heparin-binding growth factors.
1991,
Pubmed
Larsen,
Graphite powder as an alternative or supplement to reversed-phase material for desalting and concentration of peptide mixtures prior to matrix-assisted laser desorption/ionization-mass spectrometry.
2002,
Pubmed
Li,
Epidermal secretions of terrestrial flatworms and slugs: Lehmannia valentiana mucus contains matrilin-like proteins.
2007,
Pubmed
Marsh,
Simple charring method for determination of lipids.
1966,
Pubmed
Park,
Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasilurus asotus.
1998,
Pubmed
Parseghian,
Beyond the walls of the nucleus: the role of histones in cellular signaling and innate immunity.
2006,
Pubmed
Richards,
Histone H1: an antimicrobial protein of Atlantic salmon (Salmo salar).
2001,
Pubmed
Santos,
Morphology and tenacity of the tube foot disc of three common European sea urchin species: a comparative study.
2006,
Pubmed
,
Echinobase
Santos,
Adhesion of echinoderm tube feet to rough surfaces.
2005,
Pubmed
,
Echinobase
Schmiedeke,
Histones have high affinity for the glomerular basement membrane. Relevance for immune complex formation in lupus nephritis.
1989,
Pubmed
Silverman,
Understanding marine mussel adhesion.
2007,
Pubmed
Smith,
Differences in the Composition of Adhesive and Non-Adhesive Mucus From the Limpet Lottia limatula.
1999,
Pubmed
Smith,
Biochemical differences between trail mucus and adhesive mucus from marsh periwinkle snails.
2002,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Stewart,
The tube cement of Phragmatopoma californica: a solid foam.
2004,
Pubmed
Tatham,
Elastomeric proteins: biological roles, structures and mechanisms.
2000,
Pubmed
Waite,
Adhesion a la moule.
2002,
Pubmed
Waite,
Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline.
1981,
Pubmed
Winger,
Identification of intra- and intermolecular disulphide bonding in the plant mitochondrial proteome by diagonal gel electrophoresis.
2007,
Pubmed
