Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Sci Total Environ
2009 Mar 15;4077:2209-15. doi: 10.1016/j.scitotenv.2008.12.054.
Show Gene links
Show Anatomy links
Effects of 17beta-estradiol, and its metabolite, 4-hydroxyestradiol on fertilization, embryo development and oxidative DNA damage in sand dollar (Dendraster excentricus) sperm.
Rempel MA
,
Hester B
,
Deharo H
,
Hong H
,
Wang Y
,
Schlenk D
.
???displayArticle.abstract???
Oxidative compounds have been demonstrated to decrease the fertilization capability and viability of offspring of treated spermatozoa. As estrogen and its hydroxylated metabolites readily undergo redox cycling, this study was undertaken to determine if estrogens and other oxidants could damage DNA and impair sperm function. Sperm was preexposed to either 17beta-estradiol (E2), 4-hydroxyestradiol (4OHE2) or the oxidant t-butyl hydroperoxide (t-BOOH), and allowed to fertilize untreated eggs. The fertilization rates and development of the larvae were assessed, as well as the amount of 8-oxodeoxyguanosine (8-oxodG) as an indication of oxidative DNA damage. All compounds caused significant decreases in fertilization and increases in pathological abnormalities in offspring, with 4OHE2 being the most toxic. Treatment with 4OHE2 caused a significant increase of 8-oxodG, but E2 failed to show any effect. Pathological abnormalities were significantly correlated (r(2)=0.44, p< or =0.05) with 8-oxodG levels in sperm treated with t-BOOH and 4OHE2, but not E2. 8-OxodG levels also were somewhat weakly correlated with impaired fertilization in 4OHE2-treated sperm (r(2)=0.33, p< or =0.05). The results indicate that biotransformation of E2 to 4OHE2 enhances oxidative damage of DNA in sperm, which can reduce fertilization and impair embryonic development, but other mechanisms of action may also contribute to these effects.
Adam,
DNA damage by tert-butoxyl radicals generated in the photolysis of a water-soluble, DNA-binding peroxyester acting as a radical source.
1998, Pubmed
Adam,
DNA damage by tert-butoxyl radicals generated in the photolysis of a water-soluble, DNA-binding peroxyester acting as a radical source.
1998,
Pubmed
Ahmadi,
Fertilizing ability of DNA-damaged spermatozoa.
1999,
Pubmed
Aitken,
Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function.
1995,
Pubmed
Ashwood-Smith,
DNA repair by oocytes.
1996,
Pubmed
Baker,
Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations.
2004,
Pubmed
Balasinor,
Effect of tamoxifen on sperm fertilising ability and preimplantation embryo development.
2001,
Pubmed
Baldi,
A novel functional estrogen receptor on human sperm membrane interferes with progesterone effects.
2000,
Pubmed
Carreau,
Aromatase expression in male germ cells.
2001,
Pubmed
Cavalieri,
Catechol estrogen metabolites and conjugates in different regions of the prostate of Noble rats treated with 4-hydroxyestradiol: implications for estrogen-induced initiation of prostate cancer.
2002,
Pubmed
Choi,
Adaptation to oxidative stress: quinone-mediated protection of signaling in rat lung epithelial L2 cells.
1997,
Pubmed
Das,
Differential spatiotemporal regulation of lactoferrin and progesterone receptor genes in the mouse uterus by primary estrogen, catechol estrogen, and xenoestrogen.
1998,
Pubmed
Dietrich,
Effects of UV irradiation and hydrogen peroxide on DNA fragmentation, motility and fertilizing ability of rainbow trout (Oncorhynchus mykiss) spermatozoa.
2005,
Pubmed
Hong,
Identification and quantification of a guanine-thymine intrastrand cross-link lesion induced by Cu(II)/H2O2/ascorbate.
2006,
Pubmed
Lapshina,
Cytotoxic and genotoxic effects of tert-butyl hydroperoxide on Chinese hamster B14 cells.
2005,
Pubmed
Latour,
Oxidative DNA damage by t-butyl hydroperoxide causes DNA single strand breaks which is not linked to cell lysis. A mechanistic study in freshly isolated rat hepatocytes.
1995,
Pubmed
Lazzé,
Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells.
2003,
Pubmed
Lee,
Characterization of the oxidative metabolites of 17beta-estradiol and estrone formed by 15 selectively expressed human cytochrome p450 isoforms.
2003,
Pubmed
Lewis,
DNA damage to spermatozoa has impacts on fertilization and pregnancy.
2005,
Pubmed
Lu,
Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina.
2005,
Pubmed
,
Echinobase
Luconi,
Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens.
2004,
Pubmed
Luconi,
Identification and characterization of a novel functional estrogen receptor on human sperm membrane that interferes with progesterone effects.
1999,
Pubmed
Markides,
Concentration dependence of prooxidant and antioxidant properties of catecholestrogens.
1998,
Pubmed
Markides,
Specific binding of 4-hydroxyestradiol to mouse uterine protein: evidence of a physiological role for 4-hydroxyestradiol.
2005,
Pubmed
Mwatibo,
Estradiol disrupts sea urchin embryogenesis differently from methoxychlor.
1998,
Pubmed
,
Echinobase
Pagano,
The role of oxidative stress in developmental and reproductive toxicity of tamoxifen.
2001,
Pubmed
,
Echinobase
Rempel,
Uptake of estradiol from sediment by hornyhead turbot (Pleuronichthys verticalis) and effects on oxidative DNA damage in male gonads.
2008,
Pubmed
Roepke,
Estradiol and endocrine disrupting compounds adversely affect development of sea urchin embryos at environmentally relevant concentrations.
2005,
Pubmed
,
Echinobase
Schütze,
Binding of 2-hydroxyestradiol and 4-hydroxyestradiol to the estrogen receptor of MCF-7 cells in cytosolic extracts and in nuclei of intact cells.
1994,
Pubmed
Sestili,
Rotenone and pyruvate prevent the tert-butylhydroperoxide-induced necrosis of U937 cells and allow them to proliferate.
1999,
Pubmed
Sharma,
Sperm DNA damage and its clinical relevance in assessing reproductive outcome.
2004,
Pubmed
Valentine,
Mutagenesis by peroxy radical is dominated by transversions at deoxyguanosine: evidence for the lack of involvement of 8-oxo-dG1 and/or abasic site formation.
1998,
Pubmed
Vernet,
Antioxidant strategies in the epididymis.
2004,
Pubmed
Wellejus,
Receptor-mediated ethinylestradiol-induced oxidative DNA damage in rat testicular cells.
2002,
Pubmed
Wellejus,
Cell-specific oxidative DNA damage induced by estrogen in rat testicular cells in vitro.
2004,
Pubmed
Wu,
Localization of estrogen receptor alpha and beta RNA in germinal and nongerminal epithelia of the channel catfish testis.
2001,
Pubmed
Yao,
Inhibition of cellular enzymes by equine catechol estrogens in human breast cancer cells: specificity for glutathione S-transferase P1-1.
2002,
Pubmed
Zahid,
The greater reactivity of estradiol-3,4-quinone vs estradiol-2,3-quinone with DNA in the formation of depurinating adducts: implications for tumor-initiating activity.
2006,
Pubmed
Zhou,
Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress.
2006,
Pubmed