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Zygote
2008 Nov 01;164:355-61. doi: 10.1017/S096719940800484X.
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Exogenous hyalin and sea urchin gastrulation. Part III: biological activity of hyalin isolated from Lytechinus pictus embryos.
Contreras A
,
Vitale J
,
Hutchins-Carroll V
,
Carroll EJ
,
Oppenheimer SB
.
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Hyalin is a large glycoprotein, consisting of the hyalin repeat domain and non-repeated regions, and is the major component of the hyaline layer in the early sea urchin embryo of Strongylocentrotus purpuratus. The hyalin repeat domain has been identified in proteins from organisms as diverse as bacteria, sea urchins, worms, flies, mice and humans. While the specific function of hyalin and the hyalin repeat domain is incompletely understood, many studies suggest that it has a functional role in adhesive interactions. In part I of this series, we showed that hyalin isolated from the sea urchin S. purpuratus blocked archenteron elongation and attachment to the blastocoel roof occurring during gastrulation in S. purpuratus embryos, (Razinia et al., 2007). The cellular interactions that occur in the sea urchin, recognized by the U.S. National Institutes of Health as a model system, may provide insights into adhesive interactions that occur in human health and disease. In part II of this series, we showed that S. purpuratus hyalin heterospecifically blocked archenteron-ectoderm interaction in Lytechinus pictus embryos (Alvarez et al., 2007). In the current study, we have isolated hyalin from the sea urchin L. pictus and demonstrated that L. pictus hyalin homospecifically blocks archenteron-ectoderm interaction, suggesting a general role for this glycoprotein in mediating a specific set of adhesive interactions. We also found one major difference in hyalin activity in the two sea urchin species involving hyalin influence on gastrulation invagination.
Adelson,
Sea urchin morphogenesis and cell-hyalin adhesion are perturbed by a monoclonal antibody specific for hyalin.
1988, Pubmed,
Echinobase
Adelson,
Sea urchin morphogenesis and cell-hyalin adhesion are perturbed by a monoclonal antibody specific for hyalin.
1988,
Pubmed
,
Echinobase
Alvarez,
Exogenous hyalin and sea urchin gastrulation, Part II: hyalin, an interspecies cell adhesion molecule.
2008,
Pubmed
,
Echinobase
Callebaut,
HYR, an extracellular module involved in cellular adhesion and related to the immunoglobulin-like fold.
2000,
Pubmed
Citkowitz,
The hyaline layer: its isolation and role in echinoderm development.
1971,
Pubmed
,
Echinobase
Coyle-Thompson,
A novel approach to study adhesion mechanisms by isolation of the interacting system.
2005,
Pubmed
,
Echinobase
Davidson,
The sea urchin genome: where will it lead us?
2006,
Pubmed
,
Echinobase
Edelman,
CAMs and Igs: cell adhesion and the evolutionary origins of immunity.
1987,
Pubmed
Fink,
Three cell recognition changes accompany the ingression of sea urchin primary mesenchyme cells.
1985,
Pubmed
,
Echinobase
Gray,
Resolution and characterization of a major protein of the sea urchin hyaline layer.
1986,
Pubmed
,
Echinobase
Hoodbhoy,
Relationship between p62 and p56, two proteins of the mammalian cortical granule envelope, and hyalin, the major component of the echinoderm hyaline layer, in hamsters.
2000,
Pubmed
,
Echinobase
Hylander,
An ultrastructural immunocytochemical localization of hyalin in the sea urchin egg.
1982,
Pubmed
,
Echinobase
Itza,
Septate junctions mediate the barrier to paracellular permeability in sea urchin embryos.
2005,
Pubmed
,
Echinobase
Jaffe,
Quantitative microinjection of oocytes, eggs, and embryos.
2004,
Pubmed
Justice,
A calcium-insoluble 6.4 S protein derived from sea urchin cortical granule exudate.
1988,
Pubmed
,
Echinobase
Justice,
A 9.6 S protein is the third calcium-insoluble component of the sea urchin hyaline layer.
1992,
Pubmed
,
Echinobase
Khurrum,
Carbohydrate involvement in cellular interactions in sea urchin gastrulation.
2004,
Pubmed
,
Echinobase
Latham,
Accessing the embryo interior without microinjection.
1998,
Pubmed
,
Echinobase
McClay,
Embryo dissociation, cell isolation, and cell reassociation.
1986,
Pubmed
,
Echinobase
Merril,
Gel protein stains: silver stain.
1984,
Pubmed
Razinia,
Microplate assay for quantifying developmental morphologies: effects of exogenous hyalin on sea urchin gastrulation.
2007,
Pubmed
,
Echinobase
Sajadi,
Cyclodextrin, a probe for studying adhesive interactions.
2007,
Pubmed
,
Echinobase
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Stephens,
Some properties of hyalin: the calcium-insoluble protein of the hyaline layer of the sea urchin egg.
1970,
Pubmed
,
Echinobase
Vater,
Purification and characterization of a cortical secretory vesicle membrane fraction.
1989,
Pubmed
,
Echinobase
Wessel,
A molecular analysis of hyalin--a substrate for cell adhesion in the hyaline layer of the sea urchin embryo.
1998,
Pubmed
,
Echinobase
Whittaker,
The echinoderm adhesome.
2006,
Pubmed
,
Echinobase
