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Intramolecular strain coordinates kinesin stepping behavior along microtubules.
Yildiz A
,
Tomishige M
,
Gennerich A
,
Vale RD
.
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Kinesin advances 8 nm along a microtubule per ATP hydrolyzed, but the mechanism responsible for coordinating the enzymatic cycles of kinesin''s two identical motor domains remains unresolved. Here, we have tested whether such coordination is mediated by intramolecular tension generated by the "neck linkers," mechanical elements that span between the motor domains. When tension is reduced by extending the neck linkers with artificial peptides, the coupling between ATP hydrolysis and forward stepping is impaired and motor''s velocity decreases as a consequence. However, speed recovers to nearly normal levels when external tension is applied by an optical trap. Remarkably, external load also induces bidirectional stepping of an immotile kinesin that lacks its mechanical element (neck linker) and fuel (ATP). Our results indicate that the kinesin motor domain senses and responds to strain in a manner that facilitates its plus-end-directed stepping and communication between its two motor domains.
Alonso,
An ATP gate controls tubulin binding by the tethered head of kinesin-1.
2007, Pubmed
Alonso,
An ATP gate controls tubulin binding by the tethered head of kinesin-1.
2007,
Pubmed
Asbury,
Kinesin moves by an asymmetric hand-over-hand mechanism.
2003,
Pubmed
Block,
Kinesin motor mechanics: binding, stepping, tracking, gating, and limping.
2007,
Pubmed
Block,
Probing the kinesin reaction cycle with a 2D optical force clamp.
2003,
Pubmed
Carter,
Mechanics of the kinesin step.
2005,
Pubmed
Carter,
Kinesin's moonwalk.
2006,
Pubmed
Crevel,
What kinesin does at roadblocks: the coordination mechanism for molecular walking.
2004,
Pubmed
Dixit,
Differential regulation of dynein and kinesin motor proteins by tau.
2008,
Pubmed
Gebhardt,
Myosin-V is a mechanical ratchet.
2006,
Pubmed
Gennerich,
Force-induced bidirectional stepping of cytoplasmic dynein.
2007,
Pubmed
Gilbert,
Alternating site mechanism of the kinesin ATPase.
1998,
Pubmed
Guydosh,
Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain.
2006,
Pubmed
Hackney,
Biochemistry. Processive motor movement.
2007,
Pubmed
Hackney,
Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups.
2003,
Pubmed
Hackney,
The rate-limiting step in microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains occurs while bound to the microtubule.
1994,
Pubmed
Hackney,
The tethered motor domain of a kinesin-microtubule complex catalyzes reversible synthesis of bound ATP.
2005,
Pubmed
Hancock,
Kinesin's processivity results from mechanical and chemical coordination between the ATP hydrolysis cycles of the two motor domains.
1999,
Pubmed
Hwang,
Force generation in kinesin hinges on cover-neck bundle formation.
2008,
Pubmed
Kaseda,
Alternate fast and slow stepping of a heterodimeric kinesin molecule.
2003,
Pubmed
Kawaguchi,
Nucleotide-dependent single- to double-headed binding of kinesin.
2001,
Pubmed
Klumpp,
Kinesin's second step.
2004,
Pubmed
Mori,
How kinesin waits between steps.
2007,
Pubmed
Ray,
Kinesin follows the microtubule's protofilament axis.
1993,
Pubmed
Reck-Peterson,
Single-molecule analysis of dynein processivity and stepping behavior.
2006,
Pubmed
Rice,
A structural change in the kinesin motor protein that drives motility.
1999,
Pubmed
Rice,
Thermodynamic properties of the kinesin neck-region docking to the catalytic core.
2003,
Pubmed
Rock,
Myosin VI is a processive motor with a large step size.
2001,
Pubmed
Rosenfeld,
ATP reorients the neck linker of kinesin in two sequential steps.
2001,
Pubmed
Rosenfeld,
Stepping and stretching. How kinesin uses internal strain to walk processively.
2003,
Pubmed
Schief,
Conformational changes during kinesin motility.
2001,
Pubmed
Schief,
Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism.
2004,
Pubmed
Schnitzer,
Statistical kinetics of processive enzymes.
1995,
Pubmed
Schnitzer,
Kinesin hydrolyses one ATP per 8-nm step.
1997,
Pubmed
Schuler,
Polyproline and the "spectroscopic ruler" revisited with single-molecule fluorescence.
2005,
Pubmed
Skiniotis,
Nucleotide-induced conformations in the neck region of dimeric kinesin.
2003,
Pubmed
Spudich,
Molecular motors take tension in stride.
2006,
Pubmed
Svoboda,
Direct observation of kinesin stepping by optical trapping interferometry.
1993,
Pubmed
Svoboda,
Force and velocity measured for single kinesin molecules.
1994,
Pubmed
Thorn,
Engineering the processive run length of the kinesin motor.
2000,
Pubmed
Tomishige,
Single-molecule observations of neck linker conformational changes in the kinesin motor protein.
2006,
Pubmed
Uemura,
Loading direction regulates the affinity of ADP for kinesin.
2003,
Pubmed
Vale,
The molecular motor toolbox for intracellular transport.
2003,
Pubmed
Valentine,
To step or not to step? How biochemistry and mechanics influence processivity in Kinesin and Eg5.
2007,
Pubmed
Yildiz,
Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization.
2003,
Pubmed
Yildiz,
Kinesin walks hand-over-hand.
2004,
Pubmed
