Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Dyn
2008 May 01;2375:1348-58. doi: 10.1002/dvdy.21533.
Show Gene links
Show Anatomy links
Bipolar, anastral spindle development in artificially activated sea urchin eggs.
Henson JH
,
Fried CA
,
McClellan MK
,
Ader J
,
Davis JE
,
Oldenbourg R
,
Simerly CR
.
???displayArticle.abstract???
The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar "mini-spindles" that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole.
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968, Pubmed,
Echinobase
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968,
Pubmed
,
Echinobase
Bestor,
Configurations of microtubules in artificially activated eggs of the sea urchin Lytechinus variegatus.
1982,
Pubmed
,
Echinobase
Burgess,
Site selection for the cleavage furrow at cytokinesis.
2005,
Pubmed
Chang,
Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function.
2005,
Pubmed
Chui,
Roles of two homotetrameric kinesins in sea urchin embryonic cell division.
2000,
Pubmed
,
Echinobase
Compton,
Spindle assembly in animal cells.
2000,
Pubmed
de Saint Phalle,
Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos.
1998,
Pubmed
Epel,
An analysis of the partial metabolic derepression of sea urchin eggs by ammonia: the existence of independent pathways.
1974,
Pubmed
,
Echinobase
Fant,
Cell and molecular biology of spindle poles and NuMA.
2004,
Pubmed
Ganem,
The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK.
2004,
Pubmed
Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
Goshima,
Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.
2005,
Pubmed
Goshima,
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line.
2003,
Pubmed
Harris,
Conditions for assembly of tubulin-based structures in unfertilized sea urchin eggs. Spirals, monasters and cytasters.
1992,
Pubmed
,
Echinobase
Heald,
Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization.
1997,
Pubmed
Holy,
Differential behavior of centrosomes in unequally dividing blastomeres during fourth cleavage of sea urchin embryos.
1991,
Pubmed
,
Echinobase
Holy,
Recruitment of maternal material during assembly of the zygote centrosome in fertilized sea urchin eggs.
1997,
Pubmed
,
Echinobase
Holy,
Spindle pole centrosomes of sea urchin embryos are partially composed of material recruited from maternal stores.
1991,
Pubmed
,
Echinobase
Kallenbach,
Origin and maturation of centrioles in association with the nuclear envelope in hypertonic-stressed sea urchin eggs.
1982,
Pubmed
,
Echinobase
Kapoor,
Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.
2000,
Pubmed
Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
Karsenti,
The mitotic spindle and actin tails.
2004,
Pubmed
Kuriyama,
Microtubule cycles in oocytes of the surf clam, Spisula solidissima: an immunofluorescence study.
1986,
Pubmed
Kuriyama,
Cytasters induced within unfertilized sea-urchin eggs.
1983,
Pubmed
,
Echinobase
Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed
,
Echinobase
Mahoney,
Making microtubules and mitotic spindles in cells without functional centrosomes.
2006,
Pubmed
Maliga,
Small-molecule and mutational analysis of allosteric Eg5 inhibition by monastrol.
2006,
Pubmed
Manning,
The kinesin-13 proteins Kif2a, Kif2b, and Kif2c/MCAK have distinct roles during mitosis in human cells.
2007,
Pubmed
Mazia,
Chromosome cycles turned on in unfertilized sea urchin eggs exposed to NH4OH.
1974,
Pubmed
,
Echinobase
Mazia,
DNA synthesis turned on in unfertilized sea urchin eggs by treatment with NH4OH.
1974,
Pubmed
,
Echinobase
McNally,
Katanin controls mitotic and meiotic spindle length.
2006,
Pubmed
Merdes,
Pathways of spindle pole formation: different mechanisms; conserved components.
1997,
Pubmed
Mitchison,
Bipolarization and poleward flux correlate during Xenopus extract spindle assembly.
2004,
Pubmed
Miyamoto,
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.
2004,
Pubmed
Miyazaki,
Role of microtubules and centrosomes in the eccentric relocation of the germinal vesicle upon meiosis reinitiation in sea-cucumber oocytes.
2005,
Pubmed
,
Echinobase
Moy,
Cytasters from sea urchin eggs parthenogenetically activated by procaine.
1977,
Pubmed
,
Echinobase
O'Connell,
Cooperative mechanisms of mitotic spindle formation.
2007,
Pubmed
Oldenbourg,
New polarized light microscope with precision universal compensator.
1995,
Pubmed
Paweletz,
Fine structure of the mitotic cycle of unfertilized sea urchin eggs activated by ammoniacal sea water.
1979,
Pubmed
,
Echinobase
Paweletz,
The centrosome cycle in the mitotic cycle of sea urchin eggs.
1984,
Pubmed
,
Echinobase
Rebollo,
Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes.
2004,
Pubmed
Sachs,
A cytological study of artificial parthenogenesis in the sea urchin Arbacia punctulata.
1970,
Pubmed
,
Echinobase
Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
Schatten,
Activation of maternal centrosomes in unfertilized sea urchin eggs.
1992,
Pubmed
,
Echinobase
Schatten,
Intracellular pH shift leads to microtubule assembly and microtubule-mediated motility during sea urchin fertilization: correlations between elevated intracellular pH and microtubule activity and depressed intracellular pH and microtubule disassembly.
1985,
Pubmed
,
Echinobase
Schatten,
Behavior of centrosomes during fertilization and cell division in mouse oocytes and in sea urchin eggs.
1986,
Pubmed
,
Echinobase
Scholey,
Cell division.
2003,
Pubmed
Sharp,
The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles.
1999,
Pubmed
Shen,
Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg.
1978,
Pubmed
,
Echinobase
Shirai,
Dynamics of Mitotic Apparatus Formation and Tubulin Content during Oocyte Maturation in Starfish: (tubulin/starfish/oocyte maturation/cytoskeleton/meiosis).
1990,
Pubmed
,
Echinobase
Sluder,
Experimental separation of pronuclei in fertilized sea urchin eggs: chromosomes do not organize a spindle in the absence of centrosomes.
1985,
Pubmed
,
Echinobase
Sluder,
Reproductive capacity of sea urchin centrosomes without centrioles.
1989,
Pubmed
,
Echinobase
Srayko,
Katanin disrupts the microtubule lattice and increases polymer number in C. elegans meiosis.
2006,
Pubmed
Sun,
Role of NuMA in vertebrate cells: review of an intriguing multifunctional protein.
2006,
Pubmed
Theurkauf,
Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein.
1992,
Pubmed
Thompson-Coffe,
Cold-treated centrosome: isolation of centrosomes from mitotic sea urchin eggs, production of an anticentrosomal antibody, and novel ultrastructural imaging.
1996,
Pubmed
,
Echinobase
von Ledebur-Villiger,
Cytology and nucleic acid synthesis of parthenogenetically activated sea urchin eggs.
1972,
Pubmed
,
Echinobase
Voronina,
Cyclin B synthesis is required for sea urchin oocyte maturation.
2003,
Pubmed
,
Echinobase
Wordeman,
Microtubule-depolymerizing kinesins.
2005,
Pubmed
Zhu,
Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference.
2005,
Pubmed
