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Protoplasma
2007 Jan 01;2321-2:121-30. doi: 10.1007/s00709-007-0273-8.
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Ultrastructural and biochemical analysis of a new mutation in Chlamydomonas reinhardtii affecting the central pair apparatus.
Vucica Y
,
Diener DR
,
Rosenbaum JL
,
Koutoulis A
.
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We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.
Adams,
Central-pair microtubular complex of Chlamydomonas flagella: polypeptide composition as revealed by analysis of mutants.
1981, Pubmed
Adams,
Central-pair microtubular complex of Chlamydomonas flagella: polypeptide composition as revealed by analysis of mutants.
1981,
Pubmed
Bernstein,
A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum.
1994,
Pubmed
Bernstein,
Kinesin-like proteins in the flagella of Chlamydomonas.
1994,
Pubmed
Dentler,
Flagellar elongation and shortening in Chlamydomonas. III. structures attached to the tips of flagellar microtubules and their relationship to the directionality of flagellar microtubule assembly.
1977,
Pubmed
Dutcher,
Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii.
1984,
Pubmed
Fernández,
Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.
1989,
Pubmed
Fox,
Kinesin-related proteins in eukaryotic flagella.
1994,
Pubmed
Gaillard,
Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP).
2001,
Pubmed
Habermacher,
Regulation of flagellar dynein by phosphorylation of a 138-kD inner arm dynein intermediate chain.
1997,
Pubmed
Hoops,
Outer doublet heterogeneity reveals structural polarity related to beat direction in Chlamydomonas flagella.
1983,
Pubmed
Hosokawa,
Bending motion of Chlamydomonas axonemes after extrusion of central-pair microtubules.
1987,
Pubmed
Howard,
Regulation of Chlamydomonas flagellar dynein by an axonemal protein kinase.
1994,
Pubmed
Huang,
Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function.
1982,
Pubmed
Johnson,
Polarity of flagellar assembly in Chlamydomonas.
1992,
Pubmed
Johnson,
Localization of a kinesin-related protein to the central pair apparatus of the Chlamydomonas reinhardtii flagellum.
1994,
Pubmed
Kamiya,
Extrusion and Rotation of the central-pair microtubules in detergent-treated Chlamydomonas flagella.
1982,
Pubmed
Kindle,
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
1990,
Pubmed
Koutoulis,
The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex.
1997,
Pubmed
LEVINE,
The genetics and cytology of Chlamydomonas.
1960,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Mitchell,
Orientation of the central pair complex during flagellar bend formation in Chlamydomonas.
2003,
Pubmed
Mitchell,
Bend propagation drives central pair rotation in Chlamydomonas reinhardtii flagella.
2004,
Pubmed
Mitchell,
Characterization of a Chlamydomonas insertional mutant that disrupts flagellar central pair microtubule-associated structures.
1999,
Pubmed
Mitchell,
ATP production in Chlamydomonas reinhardtii flagella by glycolytic enzymes.
2005,
Pubmed
Omoto,
Functionally significant central-pair rotation in a primitive eukaryotic flagellum.
1981,
Pubmed
Omoto,
The pair of central tubules rotates during ciliary beat in Paramecium.
1979,
Pubmed
Pazour,
Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii.
1995,
Pubmed
Piperno,
Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii.
1977,
Pubmed
Porter,
The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility.
2000,
Pubmed
Porter,
Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms.
1992,
Pubmed
Ringo,
Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas.
1967,
Pubmed
Rupp,
The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility.
2001,
Pubmed
SAGER,
Nutritional studies with Chlamydomonas reinhardi.
1953,
Pubmed
Smith,
PF20 gene product contains WD repeats and localizes to the intermicrotubule bridges in Chlamydomonas flagella.
1997,
Pubmed
Smith,
The radial spokes and central apparatus: mechano-chemical transducers that regulate flagellar motility.
2004,
Pubmed
Smith,
The role of central apparatus components in flagellar motility and microtubule assembly.
1997,
Pubmed
Smith,
PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella.
1996,
Pubmed
Stephens,
Retention of ciliary ninefold structure after removal of microtubules.
1989,
Pubmed
,
Echinobase
Tam,
Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.
1993,
Pubmed
Wargo,
Asymmetry of the central apparatus defines the location of active microtubule sliding in Chlamydomonas flagella.
2003,
Pubmed
Wilkerson,
The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly.
1995,
Pubmed
Witman,
Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.
1972,
Pubmed
Witman,
Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.
1978,
Pubmed
Yang,
Protein phosphatases PP1 and PP2A are located in distinct positions in the Chlamydomonas flagellar axoneme.
2000,
Pubmed
Yang,
Casein kinase I is anchored on axonemal doublet microtubules and regulates flagellar dynein phosphorylation and activity.
2000,
Pubmed
Yokoyama,
Regulation of flagellar dynein activity by a central pair kinesin.
2004,
Pubmed
Zhang,
Cpc1, a Chlamydomonas central pair protein with an adenylate kinase domain.
2004,
Pubmed