ECB-ART-39855
Mol Biol Cell
2006 Sep 01;179:4093-104. doi: 10.1091/mbc.e06-02-0119.
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A global, myosin light chain kinase-dependent increase in myosin II contractility accompanies the metaphase-anaphase transition in sea urchin eggs.
Lucero A
,
Stack C
,
Bresnick AR
,
Shuster CB
.
???displayArticle.abstract???
Myosin II is the force-generating motor for cytokinesis, and although it is accepted that myosin contractility is greatest at the cell equator, the temporal and spatial cues that direct equatorial contractility are not known. Dividing sea urchin eggs were placed under compression to study myosin II-based contractile dynamics, and cells manipulated in this manner underwent an abrupt, global increase in cortical contractility concomitant with the metaphase-anaphase transition, followed by a brief relaxation and the onset of furrowing. Prefurrow cortical contractility both preceded and was independent of astral microtubule elongation, suggesting that the initial activation of myosin II preceded cleavage plane specification. The initial rise in contractility required myosin light chain kinase but not Rho-kinase, but both signaling pathways were required for successful cytokinesis. Last, mobilization of intracellular calcium during metaphase induced a contractile response, suggesting that calcium transients may be partially responsible for the timing of this initial contractile event. Together, these findings suggest that myosin II-based contractility is initiated at the metaphase-anaphase transition by Ca2+-dependent myosin light chain kinase (MLCK) activity and is maintained through cytokinesis by both MLCK- and Rho-dependent signaling. Moreover, the signals that initiate myosin II contractility respond to specific cell cycle transitions independently of the microtubule-dependent cleavage stimulus.
???displayArticle.pubmedLink??? 16837551
???displayArticle.pmcLink??? PMC1593176
???displayArticle.link??? Mol Biol Cell
???displayArticle.grants??? [+]
R25 GM061222 NIGMS NIH HHS , U56 CA096286 NCI NIH HHS , U54 CA132383 NCI NIH HHS , S06 GM008136 NIGMS NIH HHS , GM61222 NIGMS NIH HHS , GM08136 NIGMS NIH HHS
Genes referenced: cep152 LOC100887844 LOC100893746 LOC115919910 LOC576786 LOC581963
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