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Proc Natl Acad Sci U S A
2005 Nov 01;10244:15889-94. doi: 10.1073/pnas.0508274102.
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3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography.
Nicastro D
,
McIntosh JR
,
Baumeister W
.
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We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state. The tomographic reconstructions provide information at a resolution better than 6 nm about the in situ arrangements of macromolecules that are key for flagellar motility. We have visualized the heptameric rings of the motor domains in the outer dynein arm complex and determined that they lie parallel to the plane that contains the axes of neighboring flagellar microtubules. Both the material associated with the central pair of microtubules and the radial spokes display a plane of symmetry that helps to explain the planar beat pattern of these flagella. Cryo-electron tomography has proven to be a powerful technique for helping us understand the relationships between flagellar structure and function and the design of macromolecular machines in situ.
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