ECB-ART-39587
Protoplasma
2005 Oct 01;2253-4:169-84. doi: 10.1007/s00709-005-0094-6.
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Redundant mechanisms for anaphase chromosome movements: crane-fly spermatocyte spindles normally use actin filaments but also can function without them.
Fabian L
,
Forer A
.
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Actin inhibitors block or slow anaphase chromosome movements in crane-fly spermatocytes, but stopping of movement is only temporary; we assumed that cells adapt to loss of actin by switching to mechanism(s) involving only microtubules. To test this, we produced actin-filament-free spindles: we added latrunculin B during prometaphase, 9-80 min before anaphase, after which chromosomes generally moved normally during anaphase. We confirmed the absence of actin filaments by staining with fluorescent phalloidin and by showing that cytochalasin D had no effect on chromosome movement. Thus, actin filaments are involved in normal anaphase movements, but in vivo, spindles nonetheless can function normally without them. We tested whether chromosome movements in actin-filament-free spindles arise via microtubules by challenging such spindles with anti-myosin drugs. Y-27632 and BDM (2,3-butanedione monoxime), inhibitors that affect myosin at different regulatory levels, blocked chromosome movement in normal spindles and in actin-filament-free spindles. We tested whether BDM has side effects on microtubule motors. BDM had no effect on ciliary and sperm motility or on ATPase activity of isolated ciliary axonemes, and thus it does not directly block dynein. Nor does it block kinesin, assayed by a microtubule sliding assay. BDM could conceivably indirectly affect these microtubule motors, though it is unlikely that it would have the same side effect on the motors as Y-27632. Since BDM and Y-27632 both affect chromosome movement in the same way, it would seem that both affect spindle myosin; this suggests that spindle myosin interacts with kinetochore microtubules, either directly or via an intermediate component.
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Genes referenced: dnah3 LOC581395 LOC590297
References [+] :
Adames,
Evidence for polewards forces on chromosome arms during anaphase.
1996, Pubmed
Adames, Evidence for polewards forces on chromosome arms during anaphase. 1996, Pubmed
Ahmad, Motor proteins regulate force interactions between microtubules and microfilaments in the axon. 2000, Pubmed
Allen, Effects of 2,3-butanedione monoxime (BDM) on calcium channels expressed in Xenopus oocytes. 1998, Pubmed
Antin, Taxol induces postmitotic myoblasts to assemble interdigitating microtubule-myosin arrays that exclude actin filaments. 1981, Pubmed
Ayscough, Use of latrunculin-A, an actin monomer-binding drug. 1998, Pubmed
Banks, Chromosome movement: dynein-out at the kinetochore. 2001, Pubmed
Bresnick, Molecular mechanisms of nonmuscle myosin-II regulation. 1999, Pubmed
Cao, Myosin-Va binds to and mechanochemically couples microtubules to actin filaments. 2004, Pubmed
Chaffey, Myosin, microtubules, and microfilaments: co-operation between cytoskeletal components during cambial cell division and secondary vascular differentiation in trees. 2002, Pubmed
Coue, Microtubule depolymerization promotes particle and chromosome movement in vitro. 1991, Pubmed
Coué, Inhibition of actin polymerization by latrunculin A. 1987, Pubmed
Cramer, Myosin is involved in postmitotic cell spreading. 1995, Pubmed
Czaban, Rhodamine-labelled phalloidin stains components in the chromosomal spindle fibres of crane-fly spermatocytes and Haemanthus endosperm cells. 1992, Pubmed , Echinobase
DeLuca, Purification and characterization of native conventional kinesin, HSET, and CENP-E from mitotic hela cells. 2001, Pubmed
Desai, The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. 1999, Pubmed
Desai, Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles. 1998, Pubmed
Durán, Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport. 2003, Pubmed
Forer, Structure of kinetochore fibres in crane-fly spermatocytes after irradiation with an ultraviolet microbeam: neither microtubules nor actin filaments remain in the irradiated region. 2003, Pubmed
Forer, Does 2,3-butanedione monoxime inhibit nonmuscle myosin? 2005, Pubmed
Forer, Cytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes. 1998, Pubmed
Fuchs, Bridging cytoskeletal intersections. 2001, Pubmed
Fujii, Interaction of rat brain microtubule proteins and 6S tubulin with rabbit skeletal muscle actomysin. 1979, Pubmed
Fujii, Effect of taxol on the interaction of tubulin with myosin filaments. 1984, Pubmed
Fumoto, Phosphorylation of myosin II regulatory light chain is necessary for migration of HeLa cells but not for localization of myosin II at the leading edge. 2003, Pubmed
Gallo, Actin turnover is required to prevent axon retraction driven by endogenous actomyosin contractility. 2002, Pubmed
Gibbons, Chemical dissection of cilia. 1965, Pubmed
Gibson, Isolation and characterization of 22S outer arm dynein from Tetrahymena cilia. 2000, Pubmed
Goldstein, Functional redundancy in mitotic force generation. 1993, Pubmed
Goode, Functional cooperation between the microtubule and actin cytoskeletons. 2000, Pubmed
Goshima, The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. 2003, Pubmed
Hayashi, Dense precipitate of brain tubulin with skeletal muscle myosin. 1980, Pubmed
Hely, Short-term interactions between microtubules and actin filaments underlie long-term behaviour in neuronal growth cones. 1998, Pubmed
Herrmann, Effect of 2,3-butanedione monoxime on myosin and myofibrillar ATPases. An example of an uncompetitive inhibitor. 1992, Pubmed
Higuchi, Butanedione monoxime suppresses contraction and ATPase activity of rabbit skeletal muscle. 1989, Pubmed
Huang, Direct interaction of microtubule- and actin-based transport motors. 1999, Pubmed , Echinobase
Huang, Novel suppression of an L-type calcium channel in neurones of murine dorsal root ganglia by 2,3-butanedione monoxime. 1992, Pubmed
Hwang, Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables. 2003, Pubmed
Ilagan, Effects of ultraviolet-microbeam irradiation of kinetochores in crane-fly spermatocytes. 1997, Pubmed
Jedd, Visualization of peroxisomes in living plant cells reveals acto-myosin-dependent cytoplasmic streaming and peroxisome budding. 2002, Pubmed
Kelleher, Intracellular motility: how can we all work together? 1998, Pubmed
King, The dynein microtubule motor. 2000, Pubmed
Klymkowsky, Weaving a tangled web: the interconnected cytoskeleton. 1999, Pubmed
Komis, Actomyosin is involved in the plasmolytic cycle: gliding movement of the deplasmolyzing protoplast. 2003, Pubmed
Krendel, Disassembly of actin filaments leads to increased rate and frequency of mitochondrial movement along microtubules. 1998, Pubmed , Echinobase
Kusch, Microtubule capture by the cleavage apparatus is required for proper spindle positioning in yeast. 2002, Pubmed
Kuznetsov, Actin-dependent organelle movement in squid axoplasm. 1992, Pubmed
LOWRY, Protein measurement with the Folin phenol reagent. 1951, Pubmed
LaFountain, Microtubule flux mediates poleward motion of acentric chromosome fragments during meiosis in insect spermatocytes. 2001, Pubmed
LaFountain, Direct visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes. 2004, Pubmed
Langford, Actin- and microtubule-dependent organelle motors: interrelationships between the two motility systems. 1995, Pubmed
Lantz, A class VI unconventional myosin is associated with a homologue of a microtubule-binding protein, cytoplasmic linker protein-170, in neurons and at the posterior pole of Drosophila embryos. 1998, Pubmed
Ligon, Role of microtubules and actin filaments in the movement of mitochondria in the axons and dendrites of cultured hippocampal neurons. 2000, Pubmed
Lin, Myosin drives retrograde F-actin flow in neuronal growth cones. 1996, Pubmed
Mandato, Actomyosin transports microtubules and microtubules control actomyosin recruitment during Xenopus oocyte wound healing. 2003, Pubmed
Manneville, Interaction of the actin cytoskeleton with microtubules regulates secretory organelle movement near the plasma membrane in human endothelial cells. 2003, Pubmed
Marcus, A kinesin mutant with an atypical bipolar spindle undergoes normal mitosis. 2003, Pubmed
Martin, Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport. 1999, Pubmed
McGrail, The microtubule motor cytoplasmic dynein is required for spindle orientation during germline cell divisions and oocyte differentiation in Drosophila. 1997, Pubmed
Miller, The Abl-related gene (Arg) requires its F-actin-microtubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion. 2004, Pubmed
Molchan, Actomyosin promotes cell plate alignment and late lateral expansion in Tradescantia stamen hair cells. 2002, Pubmed
Morton, Latrunculin alters the actin-monomer subunit interface to prevent polymerization. 2000, Pubmed
Nebenführ, Stop-and-go movements of plant Golgi stacks are mediated by the acto-myosin system. 1999, Pubmed
Neco, The role of myosin in vesicle transport during bovine chromaffin cell secretion. 2002, Pubmed
Orias, Tetrahymena as a laboratory organism: useful strains, cell culture, and cell line maintenance. 2000, Pubmed
Osborn, Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures. 1982, Pubmed
Ostap, 2,3-Butanedione monoxime (BDM) as a myosin inhibitor. 2002, Pubmed
Pelham, High resolution detection of mechanical forces exerted by locomoting fibroblasts on the substrate. 1999, Pubmed
Pickett-Heaps, Pac-Man does not resolve the enduring problem of anaphase chromosome movement. 2001, Pubmed
Piperno, Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii. 1977, Pubmed
Pizon, Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein. 2002, Pubmed
Platt, Retardation of fading and enhancement of intensity of immunofluorescence by p-phenylenediamine. 1983, Pubmed
Rodriguez, Conserved microtubule-actin interactions in cell movement and morphogenesis. 2003, Pubmed
Rogers, Spindle microtubules in flux. 2005, Pubmed
Rogers, Myosin cooperates with microtubule motors during organelle transport in melanophores. 1998, Pubmed
Rosenblatt, Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly. 2004, Pubmed
Royou, Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. 2004, Pubmed
Ruchhoeft, Myosin functions in Xenopus retinal ganglion cell growth cone motility in vivo. 1997, Pubmed
Samaj, Effects of myosin ATPase inhibitor 2,3-butanedione 2-monoxime on distributions of myosins, F-actin, microtubules, and cortical endoplasmic reticulum in maize root apices. 2000, Pubmed
Saul, Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes. 2004, Pubmed
Savoian, The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants. 2000, Pubmed
Schaap, Temperature effects on anaphase chromosome movement in the spermatocytes of two species of crane flies (Nephrotoma suturalis Loew and Nephrotoma ferruginea Fabricius). 1979, Pubmed
Sellin, Multiple effects of 2,3-butanedione monoxime. 1994, Pubmed
Sharp, Functional coordination of three mitotic motors in Drosophila embryos. 2000, Pubmed
Sharp, Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos. 2000, Pubmed
Shimo-Oka, Tubulin-myosin interaction. Some properties of binding between tubulin and myosin. 1980, Pubmed
Siegman, Comparison of the effects of 2,3-butanedione monoxime on force production, myosin light chain phosphorylation and chemical energy usage in intact and permeabilized smooth and skeletal muscles. 1994, Pubmed
Silverman-Gavrila, Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes. 2000, Pubmed
Silverman-Gavrila, Myosin localization during meiosis I of crane-fly spermatocytes gives indications about its role in division. 2003, Pubmed
Silverman-Gavrila, Effects of anti-myosin drugs on anaphase chromosome movement and cytokinesis in crane-fly primary spermatocytes. 2001, Pubmed
Small, Microtubules meet substrate adhesions to arrange cell polarity. 2003, Pubmed
Spector, New anti-actin drugs in the study of the organization and function of the actin cytoskeleton. 1999, Pubmed
Straight, Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor. 2003, Pubmed
Theriot, The polymerization motor. 2000, Pubmed
Titus, Caveat experimentor--is your myosin really inhibited? 2003, Pubmed
Uemura, Mechanochemical coupling of two substeps in a single myosin V motor. 2004, Pubmed
Vale, The way things move: looking under the hood of molecular motor proteins. 2000, Pubmed
Waterman-Storer, Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport. 2000, Pubmed
Waterman-Storer, Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. 1997, Pubmed
Waterman-Storer, Positive feedback interactions between microtubule and actin dynamics during cell motility. 1999, Pubmed
Weber, A microtubule-binding myosin required for nuclear anchoring and spindle assembly. 2004, Pubmed
Wilson, Evidence that kinetochore microtubules in crane-fly spermatocytes disassemble during anaphase primarily at the poleward end. 1994, Pubmed
Wilson, Effects of nanomolar taxol on crane-fly spermatocyte spindles indicate that acetylation of kinetochore microtubules can be used as a marker of poleward tubulin flux. 1997, Pubmed
Wolfrum, Myosin VIIa as a common component of cilia and microvilli. 1998, Pubmed
Wong, 'Signalling' between chromosomes in crane-fly spermatocytes studied using ultraviolet microbeam irradiation. 2003, Pubmed
Wu, Myosin Va associates with microtubule-rich domains in both interphase and dividing cells. 1998, Pubmed
Yarrow, Rapid de-localization of actin leading edge components with BDM treatment. 2003, Pubmed
Yin, Coordinated movements between autosomal half-bivalents in crane-fly spermatocytes: evidence that 'stop' signals are sent between partner half-bivalents. 1996, Pubmed
Yin, Myosin V orientates the mitotic spindle in yeast. 2000, Pubmed
Yvon, Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells. 2001, Pubmed
Yvon, Region-specific microtubule transport in motile cells. 2000, Pubmed