Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Protein Pept Lett
2005 Jul 01;125:457-64. doi: 10.2174/0929866054395356.
Show Gene links
Show Anatomy links
Human mesotrypsin defies natural trypsin inhibitors: from passive resistance to active destruction.
Sahin-Tóth M
.
???displayArticle.abstract???
More than twenty years ago Rinderknecht et al. identified a minor trypsin isoform resistant to natural trypsin inhibitors in the human pancreatic juice. At the same time, Estell and Laskowski found that an inhibitor-resistant trypsin from the pyloric caeca of the starfish, Dermasterias imbricata rapidly hydrolyzed the reactive-site peptide bonds of trypsin inhibitors. A connection between these two seminal discoveries was made recently, when human mesotrypsin was shown to cleave the reactive-site peptide bond of the Kunitz-type soybean trypsin inhibitor, and degrade the Kazal-type pancreatic secretory trypsin inhibitor. These observations indicate that proteases specialized for the degradation of protease inhibitors are ubiquitous in metazoa, and prompt new investigations into their biological significance. Here we review the history and properties of human mesotrypsin, and discuss its function in the digestive degradation of dietary trypsin inhibitors and possible pathophysiological role in pancreatitis.
Chen,
Exclusion of anionic trypsinogen and mesotrypsinogen involvement in hereditary pancreatitis without cationic trypsinogen gene mutations.
1999, Pubmed
Chen,
Exclusion of anionic trypsinogen and mesotrypsinogen involvement in hereditary pancreatitis without cationic trypsinogen gene mutations.
1999,
Pubmed
Cottrell,
Trypsin IV, a novel agonist of protease-activated receptors 2 and 4.
2004,
Pubmed
Estell,
Dermasterias imbricata trypsin 1: an enzyme which rapidly hydrolyzes the reactive-site peptide bonds of protein trypsin inhibitors.
1980,
Pubmed
,
Echinobase
Estell,
Thermodynamics and kinetics of the hydrolysis of the reactive-site peptide bond in pancreatic trypsin inhibitor (Kunitz) by Dermasterias imbricata trypsin 1.
1980,
Pubmed
,
Echinobase
Fukuoka,
Expression and functional analysis of rat P23, a gut hormone-inducible isoform of trypsin, reveals its resistance to proteinaceous trypsin inhibitors.
2002,
Pubmed
Ghosh,
Paneth cell trypsin is the processing enzyme for human defensin-5.
2002,
Pubmed
Halangk,
Role of cathepsin B in intracellular trypsinogen activation and the onset of acute pancreatitis.
2000,
Pubmed
Hara,
Differential increases in syntheses of newly identified trypsinogen 2 isoforms by dietary protein in rat pancreas.
2004,
Pubmed
Kang,
Identification of cDNAs encoding two novel rat pancreatic serine proteases.
1992,
Pubmed
Katona,
Crystal structure reveals basis for the inhibitor resistance of human brain trypsin.
2002,
Pubmed
Kikuchi,
Site-directed mutagenesis of human pancreatic secretory trypsin inhibitor.
1989,
Pubmed
Kukor,
Human cationic trypsinogen. Arg(117) is the reactive site of an inhibitory surface loop that controls spontaneous zymogen activation.
2002,
Pubmed
Kukor,
Presence of cathepsin B in the human pancreatic secretory pathway and its role in trypsinogen activation during hereditary pancreatitis.
2002,
Pubmed
Kukor,
Human anionic trypsinogen: properties of autocatalytic activation and degradation and implications in pancreatic diseases.
2003,
Pubmed
LASKOWSKI,
Temporary inhibition of trypsin.
1953,
Pubmed
Laskowski,
What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes?
2000,
Pubmed
Le Maréchal,
Two novel severe mutations in the pancreatic secretory trypsin inhibitor gene (SPINK1) cause familial and/or hereditary pancreatitis.
2004,
Pubmed
Luthy,
Detailed mechanism of interaction of bovine -trypsin with soybean trypsin inhibitor (Kunitz). I. Stopped flow measurements.
1973,
Pubmed
Mazumdar-Leighton,
Transcriptional induction of diverse midgut trypsins in larval Agrotis ipsilon and Helicoverpa zea feeding on the soybean trypsin inhibitor.
2001,
Pubmed
Minn,
Enhanced GFAP expression in astrocytes of transgenic mice expressing the human brain-specific trypsinogen IV.
1998,
Pubmed
Nemoda,
Genetic and biochemical characterization of the E32del polymorphism in human mesotrypsinogen.
2005,
Pubmed
Nyaruhucha,
Identification and expression of the cDNA-encoding human mesotrypsin(ogen), an isoform of trypsin with inhibitor resistance.
1997,
Pubmed
Pfützer,
SPINK1/PSTI polymorphisms act as disease modifiers in familial and idiopathic chronic pancreatitis.
2000,
Pubmed
Rinderknecht,
Effects of chronic alcohol abuse on exocrine pancreatic secretion in man.
1985,
Pubmed
Rinderknecht,
Trypsinogen variants in pancreatic juice of healthy volunteers, chronic alcoholics, and patients with pancreatitis and cancer of the pancreas.
1979,
Pubmed
Rinderknecht,
Mesotrypsin: a new inhibitor-resistant protease from a zymogen in human pancreatic tissue and fluid.
1984,
Pubmed
Rowen,
The complete 685-kilobase DNA sequence of the human beta T cell receptor locus.
1996,
Pubmed
Scheele,
Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis.
1981,
Pubmed
Schick,
Hormonal stimulation in the exocrine pancreas results in coordinate and anticoordinate regulation of protein synthesis.
1984,
Pubmed
Schneider,
Sequence of tryptic cleavages in porcine pancreatic secretory inhibitor II.
1973,
Pubmed
Schneider,
Occurrence of two cleavages preceding inactivation of bovine temporary trypsin isoinhibitor A.
1974,
Pubmed
Szilágyi,
Comparative in vitro studies on native and recombinant human cationic trypsins. Cathepsin B is a possible pathological activator of trypsinogen in pancreatitis.
2001,
Pubmed
Szmola,
Human mesotrypsin is a unique digestive protease specialized for the degradation of trypsin inhibitors.
2003,
Pubmed
Tani,
Nucleotide sequence of the human pancreatic trypsinogen III cDNA.
1990,
Pubmed
Wiegand,
Cloning of the cDNA encoding human brain trypsinogen and characterization of its product.
1993,
Pubmed
Witt,
Mutations in the gene encoding the serine protease inhibitor, Kazal type 1 are associated with chronic pancreatitis.
2000,
Pubmed
Yamashita,
A tumor-suppressive role for trypsin in human cancer progression.
2003,
Pubmed
