Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time.
Berg,
Cortical granules of the sea urchin translocate early in oocyte maturation.
1997, Pubmed,
Echinobase
Berg,
Cortical granules of the sea urchin translocate early in oocyte maturation.
1997,
Pubmed
,
Echinobase
Brown,
Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy.
2003,
Pubmed
Campagnola,
Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.
2002,
Pubmed
Campagnola,
High-resolution nonlinear optical imaging of live cells by second harmonic generation.
1999,
Pubmed
Chandler,
Postfertilization growth of microvilli in the sea urchin egg: new views from eggs that have been quick-frozen, freeze-fractured, and deeply etched.
1981,
Pubmed
,
Echinobase
Fluhler,
Spectra, membrane binding, and potentiometric responses of new charge shift probes.
1985,
Pubmed
Gross,
Dual-wavelength ratiometric fluorescence measurement of the membrane dipole potential.
1994,
Pubmed
Jaffe,
The time course of cortical vesicle fusion in sea urchin eggs observed as membrane capacitance changes.
1978,
Pubmed
,
Echinobase
MacDonald,
Characteristics of self-quenching of the fluorescence of lipid-conjugated rhodamine in membranes.
1990,
Pubmed
Millard,
Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.
2004,
Pubmed
Terasaki,
Visualization of exocytosis during sea urchin egg fertilization using confocal microscopy.
1995,
Pubmed
,
Echinobase
