ECB-ART-39182
J Cell Physiol
2005 Feb 01;2022:602-7. doi: 10.1002/jcp.20157.
Show Gene links
Show Anatomy links
Cysteine-protease involved in male chromatin remodeling after fertilization co-localizes with alpha-tubulin at mitosis.
???displayArticle.abstract???
We postulated an essential role for a cysteine-protease in sea urchins sperm histones degradation which follows fertilization. We now report the purification of this enzyme, the determination of its N-terminal amino acid sequence and the localization of the protein with antibodies generated against this amino-terminal peptide. The immunofluorescence data confirmed the presence of this enzyme in the nucleus of unfertilized eggs. After fertilization labeling is observed both in female and male pronuclei suggesting a rapid recruitment of the enzyme to the male pronuclei. Interestingly, we have found that this cysteine-protease persists in the nucleus of the zygotes during S phase of the cell cycle and co-localizes with alpha-tubulin that organizes the mitotic spindle during the initial embryonic cell division.
???displayArticle.pubmedLink??? 15389576
???displayArticle.link??? J Cell Physiol
Genes referenced: LOC100887844 LOC115919910 LOC752081 LOC756768 tubgcp2