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Br J Pharmacol
2004 Aug 01;1428:1241-6. doi: 10.1038/sj.bjp.0705886.
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Triazine dyes are agonists of the NAADP receptor.
Billington RA
,
Bak J
,
Martinez-Coscolla A
,
Debidda M
,
Genazzani AA
.
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NAADP has been shown to be a potent calcium-releasing second messenger in a wide variety of cell types to date. However, research has been hampered by a lack of pharmacological agents, with which to investigate NAADP-induced calcium release, and by the molecular identity of its cellular target protein being unknown. In the present paper, the sea urchin egg model was used to investigate whether triazine dyes, which can act as nucleotide mimetics, can bind to the NAADP receptor, induce Ca(2+) release and be used for affinity chromatography of the receptor. Indeed, all the triazine dyes tested (Reactive Red 120 (RR120), Reactive Green 19 (RG19), Reactive Green 5 (RG5), Cibacron Blue 3GA and Reactive Yellow 86) displayed micromolar affinities, except for Reactive Orange 14. Furthermore, unlike NAADP, RR120, RG19 and RG5 did not bind in an irreversible manner. The compound that displayed the highest affinity, RR120, was tested in a (45)Ca(2+) efflux assay. This compound released Ca(2+) via the NAADP receptor, as shown by the ability of subthreshold NAADP concentrations to inhibit this release. Furthermore, heparin and ruthenium red were unable to block RR120-induced Ca(2+) release. We have also shown that RG5 and RG19, immobilised on resins, retain the ability to bind to the receptor, and that this interaction can be disrupted by high salt concentrations. As a proof of principle, we have shown that this can be used to partially purify the NAADP receptor by at least 75-fold. In conclusion, triazine dyes interact with the NAADP receptor, and this could be exploited in future to create a new generation of pharmacological tools to investigate this messenger and, in combination with other techniques, to purify the receptor.
Aarhus,
Activation and inactivation of Ca2+ release by NAADP+.
1996, Pubmed,
Echinobase
Aarhus,
Activation and inactivation of Ca2+ release by NAADP+.
1996,
Pubmed
,
Echinobase
Bak,
Effect of luminal and extravesicular Ca2+ on NAADP binding and release properties.
2002,
Pubmed
,
Echinobase
Berg,
Nicotinic acid adenine dinucleotide phosphate (NAADP(+)) is an essential regulator of T-lymphocyte Ca(2+)-signaling.
2000,
Pubmed
Berridge,
Solubilization of receptors for the novel Ca2+-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate.
2002,
Pubmed
,
Echinobase
Billington,
Characterization of NAADP(+) binding in sea urchin eggs.
2000,
Pubmed
,
Echinobase
Billington,
Nicotinic acid adenine dinucleotide phosphate (NAADP) is present at micromolar concentrations in sea urchin spermatozoa.
2002,
Pubmed
,
Echinobase
Bootman,
Two sulphonated dye compounds which compete for inositol 1,4, 5-trisphosphate binding to rat liver microsomes: effects on 5'-phosphatase activity.
1990,
Pubmed
Cancela,
Coordination of agonist-induced Ca2+-signalling patterns by NAADP in pancreatic acinar cells.
1999,
Pubmed
,
Echinobase
Cancela,
Transformation of local Ca2+ spikes to global Ca2+ transients: the combinatorial roles of multiple Ca2+ releasing messengers.
2002,
Pubmed
Churchill,
Sperm deliver a new second messenger: NAADP.
2003,
Pubmed
,
Echinobase
Churchill,
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
2002,
Pubmed
,
Echinobase
Dargie,
Comparison of Ca2+ mobilizing activities of cyclic ADP-ribose and inositol trisphosphate.
1990,
Pubmed
,
Echinobase
Denizli,
Dye-ligand affinity systems.
2001,
Pubmed
Dickinson,
Modulation of NAADP (nicotinic acid-adenine dinucleotide phosphate) receptors by K+ ions: evidence for multiple NAADP receptor conformations.
2003,
Pubmed
,
Echinobase
Genazzani,
Unique inactivation properties of NAADP-sensitive Ca2+ release.
1996,
Pubmed
,
Echinobase
Genazzani,
Nicotinic acid-adenine dinucleotide phosphate mobilizes Ca2+ from a thapsigargin-insensitive pool.
1996,
Pubmed
,
Echinobase
Genazzani,
NAADP: an atypical Ca2+-release messenger?
2002,
Pubmed
Genazzani,
Pharmacological properties of the Ca2+-release mechanism sensitive to NAADP in the sea urchin egg.
1997,
Pubmed
,
Echinobase
Lee,
Structural determinants of nicotinic acid adenine dinucleotide phosphate important for its calcium-mobilizing activity.
1997,
Pubmed
,
Echinobase
Lee,
A derivative of NADP mobilizes calcium stores insensitive to inositol trisphosphate and cyclic ADP-ribose.
1995,
Pubmed
,
Echinobase
Okuda,
Reactive blue 2 induces calcium oscillations in HeLa cells.
2001,
Pubmed
Patel,
Coordination of Ca2+ signalling by NAADP.
2001,
Pubmed
Patel,
Unique kinetics of nicotinic acid-adenine dinucleotide phosphate (NAADP) binding enhance the sensitivity of NAADP receptors for their ligand.
2000,
Pubmed
,
Echinobase
Xu,
Activation of the skeletal muscle Ca2+ release channel by the triazine dyes cibacron blue F3A-G and reactive red 120.
1989,
Pubmed
Yamasaki,
Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells.
2004,
Pubmed
