Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Biophys J
2004 Jan 01;861 Pt 1:346-52. doi: 10.1016/S0006-3495(04)74110-7.
Show Gene links
Show Anatomy links
Diameter oscillation of axonemes in sea-urchin sperm flagella.
Sakakibara HM
,
Kunioka Y
,
Yamada T
,
Kamimura S
.
???displayArticle.abstract???
The 9 + 2 configuration of axonemes is one of the most conserved structures of eukaryotic organelles. Evidence so far has confirmed that bending of cilia and flagella is the result of active sliding of microtubules induced by dynein arms. If the conformational change of dynein motors, which would be a key step of force generation, is occurring in a three-dimensional manner, we can easily expect that the microtubule sliding should contain some transverse component, i.e., a motion in a direction at a right angle to the longitudinal axis of axonemes. Using a modified technique of atomic force microscopy, we found such transverse motion is actually occurring in an oscillatory manner when the axonemes of sea-urchin sperm flagella were adhered onto glass substrates. The motion was adenosine triphosphate-dependent and the observed frequency of oscillation was similar to that of oscillatory sliding of microtubules that had been shown to reflect the physiological activity of dynein arms (S. Kamimura and R. Kamiya. 1989. Nature: 340:476-478; 1992. J. Cell Biol. 116:1443-1454). Maximal amplitude of the diameter oscillation was around 10 nm, which was within a range of morphological change observed with electron microscopy (F. D. Warner. 1978. J. Cell Biol. 77:R19-R26; N. C. Zanetti, D. R. Mitchell, and F. D. Warner. 1979. J. Cell Biol. 80:573-588).
Binnig,
Atomic force microscope.
1986,
Pubmed
Brokaw,
Bend propagation by a sliding filament model for flagella.
1971,
Pubmed
Brokaw,
Microtubule sliding in swimming sperm flagella: direct and indirect measurements on sea urchin and tunicate spermatozoa.
1991,
Pubmed
,
Echinobase
Brokaw,
Computer simulation of flagellar movement VIII: coordination of dynein by local curvature control can generate helical bending waves.
2002,
Pubmed
Brokaw,
Direct measurements of sliding between outer doublet microtubules in swimming sperm flagella.
1989,
Pubmed
,
Echinobase
Burgess,
Dynein structure and power stroke.
2003,
Pubmed
Carrion-Vazquez,
Mechanical and chemical unfolding of a single protein: a comparison.
1999,
Pubmed
Gibbons,
Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100.
1972,
Pubmed
,
Echinobase
Henderson,
Actin filament dynamics in living glial cells imaged by atomic force microscopy.
1992,
Pubmed
Kamimura,
High-frequency nanometre-scale vibration in 'quiescent' flagellar axonemes.
1989,
Pubmed
,
Echinobase
Kamimura,
High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin.
1992,
Pubmed
,
Echinobase
Kis,
Nanomechanics of microtubules.
2002,
Pubmed
Lindemann,
A model for flagellar motility.
1997,
Pubmed
Lindemann,
Structural-functional relationships of the dynein, spokes, and central-pair projections predicted from an analysis of the forces acting within a flagellum.
2003,
Pubmed
,
Echinobase
Mate,
Atomic-scale friction of a tungsten tip on a graphite surface.
1987,
Pubmed
Omoto,
Activation of the dynein adenosinetriphosphatase by microtubules.
1986,
Pubmed
Omoto,
The pair of central tubules rotates during ciliary beat in Paramecium.
1979,
Pubmed
Rief,
Single molecule force spectroscopy of spectrin repeats: low unfolding forces in helix bundles.
1999,
Pubmed
Rief,
Single Molecule Force Spectroscopy on Polysaccharides by Atomic Force Microscopy.
1997,
Pubmed
Sale,
Direction of active sliding of microtubules in Tetrahymena cilia.
1977,
Pubmed
Satir,
Studies on cilia. 3. Further studies on the cilium tip and a "sliding filament" model of ciliary motility.
1968,
Pubmed
Shimizu,
Activation of the dynein adenosinetriphosphatase by cross-linking to microtubules.
1989,
Pubmed
Shingyoji,
Dynein arms are oscillating force generators.
1998,
Pubmed
,
Echinobase
Shingyoji,
Local reactivation of Triton-extracted flagella by iontophoretic application of ATP.
1977,
Pubmed
,
Echinobase
Summers,
Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm.
1971,
Pubmed
,
Echinobase
Tani,
Dynein-ADP as a force-generating intermediate revealed by a rapid reactivation of flagellar axoneme.
1999,
Pubmed
,
Echinobase
Wargo,
Asymmetry of the central apparatus defines the location of active microtubule sliding in Chlamydomonas flagella.
2003,
Pubmed
Warner,
Cation-induced attachment of ciliary dynein cross-bridges.
1978,
Pubmed
Yagi,
Nanometer scale vibration in mutant axonemes of Chlamydomonas.
1994,
Pubmed
,
Echinobase
Yoshikawa,
Transverse elasticity of myofibrils of rabbit skeletal muscle studied by atomic force microscopy.
1999,
Pubmed
Zanetti,
Effects of divalent cations on dynein cross bridging and ciliary microtubule sliding.
1979,
Pubmed