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Biochem J
2004 Feb 15;378Pt 1:207-12. doi: 10.1042/BJ20030944.
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Evidence for a second peptide cleavage in the C-terminal domain of rodent intestinal mucin Muc3.
Khatri IA
,
Wang R
,
Forstner JF
.
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Rat intestinal mucin Muc3 (rMuc3), like its human homologue (MUC3) and several other membrane mucins, contains a C-terminally located SEA (sea urchin sperm protein, enterokinase and agrin) module, with an intrinsic proteolytic site sequence G downward arrow SIVV (where G downward arrow S is the glycine serine cleavage site). As shown previously [Wang, Khatri and Forstner (2002) Biochem. J. 366, 623-631], expression of the C-terminal domain of rMuc3 in COS-1 cells yields a V5 epitope-tagged N-terminal glycopeptide of 30 kDa and a Myc- and His epitope-tagged C-terminal glycopeptide of 49 kDa. The present study shows that the 49 kDa membrane-anchored fragment undergoes a further cleavage reaction which decreases its size to 30 kDa. Western blotting, pulse-chase metabolic incubations, immunoprecipitation and deglycosylation with N-glycosidase F were used to detect and identify the proteolytic products. Both the first and second cleavages are presumed to facilitate solubilization of Muc3 at the apical surface of enterocytes and/or enhance the potential for Muc3 to participate in ligand-receptor and signal transduction events for enterocyte function in vivo.
Abe,
Cleavage of Ig-Hepta at a "SEA" module and at a conserved G protein-coupled receptor proteolytic site.
2002, Pubmed
Abe,
Cleavage of Ig-Hepta at a "SEA" module and at a conserved G protein-coupled receptor proteolytic site.
2002,
Pubmed
Baruch,
The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein.
1999,
Pubmed
Carraway,
Tumor sialomucin complexes as tumor antigens and modulators of cellular interactions and proliferation.
1992,
Pubmed
Carraway,
Multiple facets of sialomucin complex/MUC4, a membrane mucin and erbb2 ligand, in tumors and tissues (Y2K update).
2000,
Pubmed
Carraway,
Cell signaling through membrane mucins.
2003,
Pubmed
Choudhury,
Human MUC4 mucin cDNA and its variants in pancreatic carcinoma.
2000,
Pubmed
Choudhury,
Alternate splicing at the 3'-end of the human pancreatic tumor-associated mucin MUC4 cDNA.
2001,
Pubmed
Déry,
Proteinase-activated receptors: novel mechanisms of signaling by serine proteases.
1998,
Pubmed
Helenius,
How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum.
1994,
Pubmed
Khatri,
SEA (sea-urchin sperm protein, enterokinase and agrin)-module cleavage, association of fragments and membrane targeting of rat intestinal mucin Muc3.
2003,
Pubmed
,
Echinobase
Khatri,
Characteristics of rodent intestinal mucin Muc3 and alterations in a mouse model of human cystic fibrosis.
2001,
Pubmed
Komatsu,
Synthesis and secretion of Muc4/sialomucin complex: implication of intracellular proteolysis.
2002,
Pubmed
Komatsu,
Reversible disruption of cell-matrix and cell-cell interactions by overexpression of sialomucin complex.
1997,
Pubmed
Ligtenberg,
Cell-associated episialin is a complex containing two proteins derived from a common precursor.
1992,
Pubmed
Longman,
Coordinated localisation of mucins and trefoil peptides in the ulcer associated cell lineage and the gastrointestinal mucosa.
2000,
Pubmed
McNeer,
Sialomucin complex in the rat respiratory tract: a model for its role in epithelial protection.
1998,
Pubmed
Moniaux,
Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin.
1999,
Pubmed
Moniaux,
Alternative splicing generates a family of putative secreted and membrane-associated MUC4 mucins.
2000,
Pubmed
Moy,
The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1.
1996,
Pubmed
,
Echinobase
Parry,
Identification of MUC1 proteolytic cleavage sites in vivo.
2001,
Pubmed
Perez-Vilar,
The structure and assembly of secreted mucins.
1999,
Pubmed
Sheng,
Molecular cloning of the transmembrane component of the 13762 mammary adenocarcinoma sialomucin complex. A new member of the epidermal growth factor superfamily.
1992,
Pubmed
Sherblom,
Cell surface sialomucin and resistance to natural cell-mediated cytotoxicity of rat mammary tumor ascites cells.
1986,
Pubmed
Smorodinsky,
Detection of a secreted MUC1/SEC protein by MUC1 isoform specific monoclonal antibodies.
1996,
Pubmed
Stacey,
LNB-TM7, a group of seven-transmembrane proteins related to family-B G-protein-coupled receptors.
2000,
Pubmed
Thim,
Effect of trefoil factors on the viscoelastic properties of mucus gels.
2002,
Pubmed
Vacquier,
The fucose sulfate polymer of egg jelly binds to sperm REJ and is the inducer of the sea urchin sperm acrosome reaction.
1997,
Pubmed
,
Echinobase
Wang,
C-terminal domain of rodent intestinal mucin Muc3 is proteolytically cleaved in the endoplasmic reticulum to generate extracellular and membrane components.
2002,
Pubmed
Wormald,
Conformational studies of oligosaccharides and glycopeptides: complementarity of NMR, X-ray crystallography, and molecular modelling.
2002,
Pubmed
Wreschner,
Generation of ligand-receptor alliances by "SEA" module-mediated cleavage of membrane-associated mucin proteins.
2002,
Pubmed
Yamamoto,
Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion.
1997,
Pubmed