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The Cdc2-cyclin B kinase has a central role in regulating the onset of M phase. In starfish oocytes, Cdc2-cyclin B begins to be activated approximately 10 min after application of maturation hormone, followed by accumulation in the nucleus then nuclear envelope breakdown. By immunofluorescence and by expressing a green fluorescent (GFP) chimera of cyclin B, we find that cyclin B is present in aggregates in the cytoplasm of immature oocytes. The aggregates disperse at approximately 10 min, suggesting that the dispersal is closely related to the activation of the kinase. Using cyclin B-GFP, the dispersion begins from the region containing the centrosomes. Extractability of Cdc2-cyclin B changes with similar kinetics during maturation. Active Cdc25 phosphatase released Cdc2-cyclin B from the detergent-insoluble fraction independently of its phosphatase activity. Live cell imaging also showed that Cdc2-cyclin B begins to accumulate in the nucleus before changes in nuclear pore permeability, consistent with Cdc2-cyclin B-induced disassembly of the pores.
Beckhelling,
Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.
2003, Pubmed
Beckhelling,
Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.
2003,
Pubmed
Beckhelling,
Localised MPF regulation in eggs.
2000,
Pubmed
,
Echinobase
Borgne,
Intra-M phase-promoting factor phosphorylation of cyclin B at the prophase/metaphase transition.
1999,
Pubmed
,
Echinobase
Favreau,
Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210.
1996,
Pubmed
Hagting,
Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal.
1999,
Pubmed
Hagting,
MPF localization is controlled by nuclear export.
1998,
Pubmed
Heim,
Improved green fluorescence.
1995,
Pubmed
HIRAMOTO,
Microinjection of the live spermatozoa into sea urchin eggs.
1962,
Pubmed
,
Echinobase
Huang,
The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells.
1999,
Pubmed
Jackman,
Active cyclin B1-Cdk1 first appears on centrosomes in prophase.
2003,
Pubmed
Jaffe,
Oocyte maturation in starfish is mediated by the beta gamma-subunit complex of a G-protein.
1993,
Pubmed
,
Echinobase
Jessus,
Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B.
1992,
Pubmed
Kiehart,
Microinjection of echinoderm eggs: apparatus and procedures.
1982,
Pubmed
,
Echinobase
Kishimoto,
Activation of MPF at meiosis reinitiation in starfish oocytes.
1999,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts.
1996,
Pubmed
Lénárt,
Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.
2003,
Pubmed
,
Echinobase
Lew,
Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control.
1996,
Pubmed
Li,
Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation.
1997,
Pubmed
Macaulay,
Differential mitotic phosphorylation of proteins of the nuclear pore complex.
1995,
Pubmed
Meijer,
Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5.
1997,
Pubmed
,
Echinobase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Okumura,
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
2002,
Pubmed
,
Echinobase
Okumura,
Initial triggering of M-phase in starfish oocytes: a possible novel component of maturation-promoting factor besides cdc2 kinase.
1996,
Pubmed
,
Echinobase
Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
,
Echinobase
Otto,
Microtubule arrays in the cortex and near the germinal vesicle of immature starfish oocytes.
1984,
Pubmed
,
Echinobase
Pérez-Mongiovi,
Nuclei and microtubule asters stimulate maturation/M phase promoting factor (MPF) activation in Xenopus eggs and egg cytoplasmic extracts.
2000,
Pubmed
Pérez-Mongiovi,
A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus.
1998,
Pubmed
Peter,
Initial activation of cyclin-B1-cdc2 kinase requires phosphorylation of cyclin B1.
2002,
Pubmed
Peters,
Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
1984,
Pubmed
Picard,
Behavior of cyclin B and cyclin B-dependent kinase during starfish oocyte meiosis reinitiation: evidence for non-identity with MPF.
1998,
Pubmed
,
Echinobase
Pines,
Cell cycle. Checkpoint on the nuclear frontier.
1999,
Pubmed
Pines,
Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.
1991,
Pubmed
Rankin,
The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm.
1997,
Pubmed
Tachibana,
The starfish egg mRNA responsible for meiosis reinitiation encodes cyclin.
1990,
Pubmed
,
Echinobase
Takizawa,
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.
2000,
Pubmed
Tang,
The SH2-containing protein-tyrosine phosphatase SH-PTP2 is required upstream of MAP kinase for early Xenopus development.
1995,
Pubmed
Tao-Cheng,
Calcium/calmodulin-dependent protein kinase II clusters in adult rat hippocampal slices.
2002,
Pubmed
Terasaki,
Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein.
1996,
Pubmed
,
Echinobase
Terasaki,
Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.
2000,
Pubmed
,
Echinobase
Terasaki,
A new model for nuclear envelope breakdown.
2001,
Pubmed
,
Echinobase
Terasaki,
Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
1994,
Pubmed
,
Echinobase
Toyoshima,
Nuclear export of cyclin B1 and its possible role in the DNA damage-induced G2 checkpoint.
1998,
Pubmed
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
Yang,
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
1998,
Pubmed
Zheng,
Functional analysis of the P box, a domain in cyclin B required for the activation of Cdc25.
1993,
Pubmed
