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J Cell Biol
1964 Dec 01;23:629-50. doi: 10.1083/jcb.23.3.629.
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IMMUNO-ELECTRON MICROSCOPE ANALYSIS OF THE SURFACE LAYERS OF THE UNFERTILISED SEA URCHIN EGG. II. LOCALISATION OF SURFACE ANTIGENS.
BAXANDALL J
,
PERLMANN P
,
AFZELIUS BA
.
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The immunological properties of the surface layers of Paracentrotus lividus eggs have been studied further by using ferritin-labelled antibody to localise specific antigenic sites. In order to detect a wider spectrum of antigenic determinants, several antisera against egg and jelly substance have been employed in combination with absorption procedures using lyophilised antigen. This use of absorbed antisera was made feasible by adding ferritin label in a second antiserum layer of ferritin-anti-gamma-globulin. Eggs were treated with antibody for short periods to detect antigenic sites without incurring structural changes (shown in previous paper) resulting from long antibody treatment. Unspecific ferritin uptake, found in pinocytotic vesicles and yolk granules, is considered in relation to yolk formation. The jelly layer, found to be immunologically heterogeneous, included one component interacting with antijelly gamma-globulin and one with antiegg gamma-globulin. The vitelline membrane proved to be rich in egg antigens (heat-stable and heat-labile). The role of this layer in specificity of fertilisation, parthenogenetic activation, and the possibility of being analogous to a basement membrane are discussed. Few antigenic sites were found on the plasma membrane with antiegg gamma-globulin. This gamma-globulin resulted in some specific labelling of cortical granules and its action is considered in relation to the permeability properties of the egg.
ALMEIDA,
THE STRUCTURE OF ANTIGEN-ANTIBODY COMPLEXES. A STUDY BY ELECTRON MICROSCOPY.
1963, Pubmed
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THE STRUCTURE OF ANTIGEN-ANTIBODY COMPLEXES. A STUDY BY ELECTRON MICROSCOPY.
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Electron microscopic studies of experimental nephritis with ferritin-conjugated antibody. The basement membranes and cisternae of visceral epithelial cells in nephritic rat glomeruli.
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IMMUNO-ELECTRON MICROSCOPE ANALYSIS OF THE SURFACE LAYERS OF THE UNFERTILISED SEA URCHIN EGG. I. EFFECTS OF THE ANTISERA ON THE CELL ULTRASTRUCTURE.
1964,
Pubmed
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Echinobase
BEAMS,
ELECTRON MICROSCOPE STUDIES ON DEVELOPING CRAYFISH OOCYTES WITH SPECIAL REFERENCE TO THE ORIGIN OF YOLK.
1963,
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BOREK,
Characterization and purification of ferritin-antibody globulin conjugates.
1961,
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BRANDT,
A consideration of the extraneous coats of the plasma membrane.
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COLWIN,
ROLE OF THE GAMETE MEMBRANES IN FERTILIZATION IN SACCOGLOSSUS KOWALEVSKII (ENTEROPNEUSTA). I. THE ACROSOMAL REGION AND ITS CHANGES IN EARLY STAGES OF FERTILIZATION.
1963,
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EASTON,
Demonstration of surface antigens and pinocytosis in mammalian cells with ferritin-antibody conjugates.
1962,
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EASTON,
Immune cytolysis: electron microscopic localization of cellular antigens with ferritin-antibody conjugates.
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Pinocytosis in the developing egg of Limnaea stagnalis L.
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Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.
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PERLMANN,
Analysis of the surface structures of the sea urchin egg by means of antibodies. II. The C- and F-antigens.
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PERLMANN,
Immunochemical analysis of the surface of the sea urchin egg; an approach to the study of fertilization.
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PERLMANN,
Analysis of the surface structures of the sea urchin egg by means of antibodies. I. Comparative study of the effects of various antisera.
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The histogenesis of basement membranes.
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Use of ferritin-conjugated antibody to localize antigen by electron microscopy.
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1963,
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