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ECB-ART-38572
Science 1963 Oct 18;1423590:389-90. doi: 10.1126/science.142.3590.389.
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MALATE DEHYDROGENASE: MULTIPLE FORMS IN SEPARATED BLASTOMERES OF SEA URCHIN EMBRYOS.

MOORE RO , VILLEE CA .


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Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.

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Genes referenced: LOC100887844 LOC115919910 LOC115925415 LOC587800