Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-38006
Dev Dyn 2003 Mar 01;2263:470-7. doi: 10.1002/dvdy.10254.
Show Gene links Show Anatomy links

Methods for introducing morpholinos into the chicken embryo.

Kos R , Tucker RP , Hall R , Duong TD , Erickson CA .


???displayArticle.abstract???
The use of antisense morpholino oligos to inhibit the translation of a target transcript has been applied recently to studies of the chicken embryo. In contrast to other developmental systems such as in frog, sea urchin, and zebrafish that permit the direct microinjection of morpholinos into a blastomere, square pulse electroporation is used to introduce fluorescently tagged morpholinos into specific populations of chick embryo cells in ovo. This article reviews the methods that have proven successful, the types of controls that are necessary when performing knockdowns of gene expression in the chick embryo, and discusses the limitations of the current technique, as well as directions for further research.

???displayArticle.pubmedLink??? 12619133
???displayArticle.link??? Dev Dyn
???displayArticle.grants??? [+]

Genes referenced: LOC100887844 ovol1