Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
2001 Nov 01;2121:7231-42. doi: 10.1128/MCB.21.21.7231-7242.2001.
Show Gene links
Show Anatomy links
Ectopic expression of DREF induces DNA synthesis, apoptosis, and unusual morphogenesis in the Drosophila eye imaginal disc: possible interaction with Polycomb and trithorax group proteins.
Hirose F
,
Ohshima N
,
Shiraki M
,
Inoue YH
,
Taguchi O
,
Nishi Y
,
Matsukage A
,
Yamaguchi M
.
???displayArticle.abstract???
The promoters of Drosophila genes encoding DNA replication-related proteins contain transcription regulatory element DRE (5''-TATCGATA) in addition to E2F recognition sites. A specific DRE-binding factor, DREF, positively regulates DRE-containing genes. In addition, it has been reported that DREF can bind to a sequence in the hsp70 scs'' chromatin boundary element that is also recognized by boundary element-associated factor, and thus DREF may participate in regulating insulator activity. To examine DREF function in vivo, we established transgenic flies in which ectopic expression of DREF was targeted to the eye imaginal discs. Adult flies expressing DREF exhibited a severe rough eye phenotype. Expression of DREF induced ectopic DNA synthesis in the cells behind the morphogenetic furrow, which are normally postmitotic, and abolished photoreceptor specifications of R1, R6, and R7. Furthermore, DREF expression caused apoptosis in the imaginal disc cells in the region where commitment to R1/R6 cells takes place, suggesting that failure of differentiation of R1/R6 photoreceptor cells might cause apoptosis. The DREF-induced rough eye phenotype was suppressed by a half-dose reduction of the E2F gene, one of the genes regulated by DREF, indicating that the DREF overexpression phenotype is useful to screen for modifiers of DREF activity. Among Polycomb/trithorax group genes, we found that a half-dose reduction of some of the trithorax group genes involved in determining chromatin structure or chromatin remodeling (brahma, moira, and osa) significantly suppressed and that reduction of Distal-less enhanced the DREF-induced rough eye phenotype. The results suggest a possibility that DREF activity might be regulated by protein complexes that play a role in modulating chromatin structure. Genetic crosses of transgenic flies expressing DREF to a collection of Drosophila deficiency stocks allowed us to identify several genomic regions, deletions of which caused enhancement or suppression of the DREF-induced rough eye phenotype. These deletions should be useful to identify novel targets of DREF and its positive or negative regulators.
Asano,
Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs.
1996, Pubmed
Asano,
Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs.
1996,
Pubmed
Biggin,
Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts.
1988,
Pubmed
Brizuela,
The Drosophila homeotic gene moira regulates expression of engrailed and HOM genes in imaginal tissues.
1997,
Pubmed
,
Echinobase
Brook,
Requirements for dE2F function in proliferating cells and in post-mitotic differentiating cells.
1996,
Pubmed
Butler,
klingon, a novel member of the Drosophila immunoglobulin superfamily, is required for the development of the R7 photoreceptor neuron.
1997,
Pubmed
Caldas,
Cell memory and cancer--the story of the trithorax and Polycomb group genes.
1999,
Pubmed
Carthew,
seven in absentia, a gene required for specification of R7 cell fate in the Drosophila eye.
1990,
Pubmed
Crook,
An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif.
1993,
Pubmed
Crosby,
The trithorax group gene moira encodes a brahma-associated putative chromatin-remodeling factor in Drosophila melanogaster.
1999,
Pubmed
,
Echinobase
Du,
Ectopic expression of dE2F and dDP induces cell proliferation and death in the Drosophila eye.
1996,
Pubmed
Duronio,
The transcription factor E2F is required for S phase during Drosophila embryogenesis.
1995,
Pubmed
Duronio,
E2F-induced S phase requires cyclin E.
1996,
Pubmed
Freeman,
Identifying targets of the rough homeobox gene of Drosophila: evidence that rhomboid functions in eye development.
1992,
Pubmed
Gerasimova,
Boundary and insulator elements in chromosomes.
1996,
Pubmed
Gerasimova,
Polycomb and trithorax group proteins mediate the function of a chromatin insulator.
1998,
Pubmed
Harrington,
Oncogenes and cell death.
1994,
Pubmed
Hart,
The scs' boundary element: characterization of boundary element-associated factors.
1997,
Pubmed
Hart,
Evidence for an antagonistic relationship between the boundary element-associated factor BEAF and the transcription factor DREF.
1999,
Pubmed
Hay,
Expression of baculovirus P35 prevents cell death in Drosophila.
1994,
Pubmed
Hirose,
Novel 8-base pair sequence (Drosophila DNA replication-related element) and specific binding factor involved in the expression of Drosophila genes for DNA polymerase alpha and proliferating cell nuclear antigen.
1993,
Pubmed
Hirose,
Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes.
1996,
Pubmed
Hirose,
Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc.
1999,
Pubmed
Kal,
The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste.
2000,
Pubmed
,
Echinobase
Kennison,
Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila.
1988,
Pubmed
Kennison,
The Polycomb and trithorax group proteins of Drosophila: trans-regulators of homeotic gene function.
1995,
Pubmed
Laney,
zeste, a nonessential gene, potently activates Ultrabithorax transcription in the Drosophila embryo.
1992,
Pubmed
Lyko,
Chromosomal elements conferring epigenetic inheritance.
1999,
Pubmed
Matsukage,
The DRE sequence TATCGATA, a putative promoter-activating element for Drosophila melanogaster cell-proliferation-related genes.
1995,
Pubmed
Messmer,
Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster.
1992,
Pubmed
Mlodzik,
The Drosophila seven-up gene, a member of the steroid receptor gene superfamily, controls photoreceptor cell fates.
1990,
Pubmed
Mlodzik,
Isolation and expression of scabrous, a gene regulating neurogenesis in Drosophila.
1990,
Pubmed
Moses,
Glass encodes a site-specific DNA-binding protein that is regulated in response to positional signals in the developing Drosophila eye.
1991,
Pubmed
Ohno,
Transcriptional regulation of the Drosophila CycA gene by the DNA replication-related element (DRE) and DRE binding factor (DREF).
1996,
Pubmed
Pirrotta,
Chromatin complexes regulating gene expression in Drosophila.
1995,
Pubmed
Robinow,
Characterization and spatial distribution of the ELAV protein during Drosophila melanogaster development.
1991,
Pubmed
Royzman,
Mutations in Drosophila DP and E2F distinguish G1-S progression from an associated transcriptional program.
1997,
Pubmed
Ryu,
Transcriptional regulation of the Drosophila-raf proto-oncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system.
1997,
Pubmed
Sawado,
The DNA replication-related element (DRE)/DRE-binding factor system is a transcriptional regulator of the Drosophila E2F gene.
1998,
Pubmed
Staehling-Hampton,
A genetic screen for modifiers of E2F in Drosophila melanogaster.
1999,
Pubmed
,
Echinobase
Takahashi,
Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis.
1999,
Pubmed
Tamkun,
brahma: a regulator of Drosophila homeotic genes structurally related to the yeast transcriptional activator SNF2/SWI2.
1992,
Pubmed
Tillib,
Trithorax- and Polycomb-group response elements within an Ultrabithorax transcription maintenance unit consist of closely situated but separable sequences.
1999,
Pubmed
Treisman,
eyelid antagonizes wingless signaling during Drosophila development and has homology to the Bright family of DNA-binding proteins.
1997,
Pubmed
Udvardy,
The 87A7 chromomere. Identification of novel chromatin structures flanking the heat shock locus that may define the boundaries of higher order domains.
1985,
Pubmed
Vázquez,
The trithorax group gene osa encodes an ARID-domain protein that genetically interacts with the brahma chromatin-remodeling factor to regulate transcription.
1999,
Pubmed
Wang,
The Drosophila caspase inhibitor DIAP1 is essential for cell survival and is negatively regulated by HID.
1999,
Pubmed
White,
Genetic control of programmed cell death in Drosophila.
1994,
Pubmed
Wilder,
Dual functions of wingless in the Drosophila leg imaginal disc.
1995,
Pubmed
Yamaguchi,
A nucleotide sequence essential for the function of DRE, a common promoter element for Drosophila DNa replication-related genes.
1995,
Pubmed
Yamaguchi,
Essential role of E2F recognition sites in regulation of the proliferating cell nuclear antigen gene promoter during Drosophila development.
1995,
Pubmed
Yamaguchi,
Ectopic expression of BEAF32A in the Drosophila eye imaginal disc inhibits differentiation of photoreceptor cells and induces apoptosis.
2001,
Pubmed
Yamaguchi,
Roles of multiple promoter elements of the proliferating cell nuclear antigen gene during Drosophila development.
1996,
Pubmed
Yamaguchi,
Ectopic expression of human p53 inhibits entry into S phase and induces apoptosis in the Drosophila eye imaginal disc.
1999,
Pubmed
