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Glycoconj J
2000 Jan 01;173 -4:205-14. doi: 10.1023/a:1026589223811.
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Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm.
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The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for egg ligands, a glycosylphosphatidylinositol (GPI)-anchored protein, and four proteins which may be involved in signal transduction. Co-immunoprecipitation revealed that at least three proteins, the speract receptor, the 63kDa GPI-anchored protein and the alpha subunit of a heterotrimeric Gs protein, are localized in the LD-DIM. This suggests that the LD-DIM fraction may be a membrane microdomain for speract-speract receptor interaction, as well as the subsequent signal transduction pathway involved in induction of sperm respiration, motility and possibly the acrosome reaction.
Bookbinder,
Identification of sea urchin sperm adenylate cyclase.
1990,
Pubmed
,
Echinobase
Brown,
Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface.
1992,
Pubmed
Brown,
Structure of detergent-resistant membrane domains: does phase separation occur in biological membranes?
1997,
Pubmed
Dangott,
Identification and partial characterization of the receptor for speract.
1984,
Pubmed
,
Echinobase
Fiedler,
Glycosphingolipid-enriched, detergent-insoluble complexes in protein sorting in epithelial cells.
1993,
Pubmed
Harder,
Caveolae, DIGs, and the dynamics of sphingolipid-cholesterol microdomains.
1997,
Pubmed
Hooper,
Detergent-insoluble glycosphingolipid/cholesterol-rich membrane domains, lipid rafts and caveolae (review).
1999,
Pubmed
Hoshi,
Novel sialosphingolipids from spermatozoa of the sea urchin Anthocidaris crassispina.
1975,
Pubmed
,
Echinobase
Ijuin,
Isolation and identification of novel sulfated and nonsulfated oligosialyl glycosphingolipids from sea urchin sperm.
1996,
Pubmed
,
Echinobase
Iwabuchi,
GM3-enriched microdomain involved in cell adhesion and signal transduction through carbohydrate-carbohydrate interaction in mouse melanoma B16 cells.
1998,
Pubmed
Iwabuchi,
Separation of "glycosphingolipid signaling domain" from caveolin-containing membrane fraction in mouse melanoma B16 cells and its role in cell adhesion coupled with signaling.
1998,
Pubmed
Kasahara,
Association of Src family tyrosine kinase Lyn with ganglioside GD3 in rat brain. Possible regulation of Lyn by glycosphingolipid in caveolae-like domains.
1997,
Pubmed
Keller,
Cholesterol is required for surface transport of influenza virus hemagglutinin.
1998,
Pubmed
Kenworthy,
Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer.
1998,
Pubmed
Kochetkov,
Isolation and structural studies of a sulfated sialosphingolipid from the sea urchin Echinocardium cordatum.
1976,
Pubmed
,
Echinobase
Köhler,
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
1976,
Pubmed
Köhler,
Continuous cultures of fused cells secreting antibody of predefined specificity.
1975,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lisanti,
Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease.
1994,
Pubmed
Maekawa,
Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (raft).
1999,
Pubmed
Mañes,
Membrane raft microdomains mediate front-rear polarity in migrating cells.
1999,
Pubmed
Nagai,
Sialosphingolipids of sea urchin eggs and spermatozoa showing a characteristic composition for species and gamete.
1975,
Pubmed
,
Echinobase
Nishioka,
Changing localizations of site-specific surface antigens during sea urchin spermiogenesis.
1987,
Pubmed
,
Echinobase
Ohta,
Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm.
1999,
Pubmed
,
Echinobase
Osawa,
Immunological evidence for the localization of sialoglycosphingolipids at the cell surface of sea urchin spermatozoa.
1975,
Pubmed
,
Echinobase
Parton,
Digging into caveolae.
1995,
Pubmed
Parton,
Caveolae and caveolins.
1996,
Pubmed
Podell,
Inhibition of sea urchin sperm acrosome reaction by antibodies directed against two sperm membrane proteins. Characterization and mechanism of action.
1984,
Pubmed
,
Echinobase
Prinetti,
Glycosphingolipid-enriched signaling domain in mouse neuroblastoma Neuro2a cells. Mechanism of ganglioside-dependent neuritogenesis.
1999,
Pubmed
Rodgers,
Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.
1996,
Pubmed
Sato,
Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods.
1998,
Pubmed
Sato,
Characterization of the antigenic specificity of four different anti-(alpha 2-->8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids.
1995,
Pubmed
Shimizu,
A mRNA for membrane form of guanylyl cyclase is expressed exclusively in the testis of the sea urchin Hemicentrotus pulcherrimus.
1996,
Pubmed
,
Echinobase
Simons,
Functional rafts in cell membranes.
1997,
Pubmed
Song,
Globoside-dependent adhesion of human embryonal carcinoma cells, based on carbohydrate-carbohydrate interaction, initiates signal transduction and induces enhanced activity of transcription factors AP1 and CREB.
1998,
Pubmed
Song,
Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.
1995,
Pubmed
SVENNERHOLM,
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
1957,
Pubmed
Tanphaichitr,
Role of a gamete-specific sulfoglycolipid immobilizing protein on mouse sperm-egg binding.
1993,
Pubmed
Trimmer,
Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm.
1985,
Pubmed
,
Echinobase
Vacquier,
Rapid immunoassays for the acrosome reaction of sea urchin sperm utilizing antibody to bindin.
1984,
Pubmed
,
Echinobase
Verkade,
Robert Feulgen Lecture 1997. Lipid microdomains and membrane trafficking in mammalian cells.
1997,
Pubmed
Yamamura,
A close association of GM3 with c-Src and Rho in GM3-enriched microdomains at the B16 melanoma cell surface membrane: a preliminary note.
1997,
Pubmed
