Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Appl Environ Microbiol
2000 Nov 01;6611:4829-33. doi: 10.1128/AEM.66.11.4829-4833.2000.
Show Gene links
Show Anatomy links
Marine planktonic archaea take up amino acids.
Ouverney CC
,
Fuhrman JA
.
???displayArticle.abstract???
Archaea are traditionally thought of as "extremophiles," but recent studies have shown that marine planktonic Archaea make up a surprisingly large percentage of ocean midwater microbial communities, up to 60% of the total prokaryotes. However, the basic physiology and contribution of Archaea to community microbial activity remain unknown. We have studied Archaea from 200-m depths of the northwest Mediterranean Sea and the Pacific Ocean near California, measuring the archaeal activity under simulated natural conditions (8 to 17 degrees C, dark and aerobic [corrected]) by means of a method called substrate tracking autoradiography fluorescence in situ hybridization (STARFISH) that simultaneously detects specific cell types by 16S rRNA probe binding and activity by microautoradiography. In the 200-m-deep Mediterranean and Pacific samples, cells binding the archaeal probes made up about 43 and 14% of the total countable cells, respectively. Our results showed that the Archaea are active in the uptake of dissolved amino acids from natural concentrations (nanomolar) with about 60% of the individuals in the archaeal communities showing measurable uptake. Bacteria showed a similar proportion of active cells. We concluded that a portion of these Archaea is heterotrophic and also appears to coexist successfully with Bacteria in the same water.
Andreasen,
Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge.
1997, Pubmed
Andreasen,
Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge.
1997,
Pubmed
Braun-Howland,
Development of a rapid method for detecting bacterial cells in situ using 16S rRNA-targeted probes.
1992,
Pubmed
Brock,
Autoradiography as a tool in microbial ecology.
1966,
Pubmed
Brunk,
A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences.
1996,
Pubmed
Cottrell,
Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter.
2000,
Pubmed
DeLong,
Archaea in coastal marine environments.
1992,
Pubmed
DeLong,
Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
1989,
Pubmed
DeLong,
Visualization and enumeration of marine planktonic archaea and bacteria by using polyribonucleotide probes and fluorescent in situ hybridization.
1999,
Pubmed
Fuhrman,
Novel major archaebacterial group from marine plankton.
1992,
Pubmed
Hershberger,
Wide diversity of Crenarchaeota.
1996,
Pubmed
Karner,
Determination of Active Marine Bacterioplankton: a Comparison of Universal 16S rRNA Probes, Autoradiography, and Nucleoid Staining.
1997,
Pubmed
Lee,
Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology.
1999,
Pubmed
Massana,
Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel.
1997,
Pubmed
Murray,
Seasonal and spatial variability of bacterial and archaeal assemblages in the coastal waters near Anvers Island, Antarctica.
1998,
Pubmed
Olsen,
Microbial ecology. Archaea, Archaea, everywhere.
1994,
Pubmed
Ouverney,
Increase in Fluorescence Intensity of 16S rRNA In Situ Hybridization in Natural Samples Treated with Chloramphenicol.
1997,
Pubmed
Ouverney,
Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ.
1999,
Pubmed
,
Echinobase
Preston,
A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov.
1996,
Pubmed
Stein,
Archaeal ubiquity.
1996,
Pubmed
Tabor,
Improved microautoradiographic method to determine individual microorganisms active in substrate uptake in natural waters.
1982,
Pubmed
