Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Characterization of a cDNA encoding RP43, a CUB-domain-containing protein from the tube of Riftia pachyptila (Vestimentifera), and distribution of its transcript.
Chamoy L
,
Nicolaï M
,
Quennedey B
,
Gaill F
,
Delachambre J
.
???displayArticle.abstract???
A major 43 kDa protein from the protective tube of Riftia pachyptila (Vestimentifera), named RP43, was partly microsequenced after isolation by SDS/PAGE from the protein fraction of tubes collected around the hydrothermal vents at the East Pacific Rise. On the basis of the partial peptide sequences obtained, experiments using reverse-transcriptase-mediated PCR and rapid amplification of cDNA ends led to the complete cDNA sequence. Analysis of deduced amino acid sequence of RP43 showed the presence of CUB domains (100-110-residue-spanning domains first reported in the complement subcomponents C1r/C1s, epidermal-growth-factor-related sea urchin protein and bone morphogenetic protein 1) that seem to be involved in protein-protein and glycosaminoglycan-protein interactions. This peculiarity strongly suggests that RP43 might have a crucial role in tightening the different elements of the worm tube. However, the absence of chitin-binding motifs inclines us to favour a role in protein-protein interactions during assembly of the tube components. The RP43 mRNA was found to be present in specific epidermal cells from the worm body wall but never in the chitin-synthesizing gland cells. This unexpected result clearly indicates that the major tube protein is synthesized in specialized areas of the outer epithelium and that at least two different tissues are involved in the synthesis of the exoskeleton.
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987, Pubmed
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987,
Pubmed
Bork,
The CUB domain. A widespread module in developmentally regulated proteins.
1993,
Pubmed
Bouhin,
Characterization of a cDNA clone encoding a glycine-rich cuticular protein of Tenebrio molitor: developmental expression and effect of a juvenile hormone analogue.
1992,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Celano,
Isolation of polyadenylated RNA from cultured cells and intact tissues.
1993,
Pubmed
Delgadillo-Reynoso,
Structural analysis of the uEGF gene in the sea urchin strongylocentrotus purpuratus reveals more similarity to vertebrate than to invertebrate genes with EGF-like repeats.
1989,
Pubmed
,
Echinobase
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Gagnon,
Primary structure of the A chain of human complement-classical-pathway enzyme C1r. N-terminal sequences and alignment of autolytic fragments and CNBr-cleavage peptides.
1985,
Pubmed
Gaill,
Aspects of life development at deep sea hydrothermal vents.
1993,
Pubmed
Higgins,
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
1988,
Pubmed
Kristiansen,
Molecular dissection of the intrinsic factor-vitamin B12 receptor, cubilin, discloses regions important for membrane association and ligand binding.
1999,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Leytus,
Nucleotide sequence of the cDNA coding for human complement C1r.
1986,
Pubmed
Manásková,
Aggregated forms of heparin-binding and non-heparin-binding proteins of boar seminal plasma and their binding properties.
1999,
Pubmed
Nielsen,
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
1997,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Romero,
The crystal structures of two spermadhesins reveal the CUB domain fold.
1997,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sanz,
The amino acid sequence of AQN-3, a carbohydrate-binding protein isolated from boar sperm. Location of disulphide bridges.
1991,
Pubmed
Sanz,
The complete primary structure of the spermadhesin AWN, a zona pellucida-binding protein isolated from boar spermatozoa.
1992,
Pubmed
Sanz,
The complete primary structure of the boar spermadhesin AQN-1, a carbohydrate-binding protein involved in fertilization.
1992,
Pubmed
Shimell,
The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1.
1991,
Pubmed
Tosi,
Complete cDNA sequence of human complement Cls and close physical linkage of the homologous genes Cls and Clr.
1987,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Töpfer-Petersen,
Spermadhesins: a new protein family. Facts, hypotheses and perspectives.
1998,
Pubmed
Wray,
Silver staining of proteins in polyacrylamide gels.
1981,
Pubmed