Environ Toxicol Pharmacol 2000 Jan 01;82:95-101. doi: 10.1016/s1382-6689(99)00037-x.

Cyanobacteria as a biosorbent of heavy metals in sewage water.

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The effect of sewage water on some physiological activities of cyanobacteria was studied. Metal-tolerant cyanobacterium (Nostoc linckia) and metal-sensitive (Nostoc rivularis) were grown at three levels of sewage water (25, 50 and 75%). The growth rate showed significant stimulation in low and moderate levels (50% for N. linckia and 25% for N. rivularis). Not only the number of cells was elevated but also, the time required to reach the exponential and the stationary phases was reduced. Also, low levels of sewage water increased chl.a content, photosynthetic O(2)-evolution, respiration and protein content. Similarly, heterocyst frequency as well as nitrogenase activity were increased in cyanobacteria grown at low and moderate levels (25 and 50% sewage). On the other hand, the high level of waste (75%) reduced growth and metabolic activities of the two species. N. linckia accumulated about 30-fold of Zn and ten-fold of Cd than those of growth medium (50% sewage water). Also, N. rivularis accumulated about ten-fold of Zn and two-fold of Cd. The distribution of Cd and Zn in cells were investigated. About 65-60% of Cd or Zn were found in pellets (sediment) as insoluble form in the two species. The soluble form (cytosolic fraction) after being fractionated on sephadex G-(75-100) revealed two peaks with molecular weights of 70-75 and 40-45 kDa. These peaks were in coincidence with Cd and Zn maxima. Nostoc rivulais showed more sensitivity to heavy metals than N. linckia, and accumulated less amount of metal-binding proteins. Nostoc linckia seems to be tolerant to heavy metals (Zn and Cd) and is able to accumulate this metal by adsorption on the pellets (cell surface) and/or through sequestration via metal-binding protein. Therefore it can be recommended it to be employed in the purification of waste contaminated with these heavy metals.

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Genes referenced: LOC115919910 LOC593358 srpl